Live imaging of mitosomes and hydrogenosomes by halotag technology

Hydrogenosomes and mitosomes represent remarkable mitochondrial adaptations in the anaerobic parasitic protists such as Trichomonas vaginalis and Giardia intestinalis, respectively. In order to provide a tool to study these organelles in the live cells, the HaloTag was fused to G. intestinalis IscU...

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Autor Principal: Sanín Peña, David Esteban; De Napoli, Maximiliano; Eshar, Shiri; Gualdrón López, Melisa; Hopp, Christine S.; Martincová, Eva; Najdrová, Vladimíra; Tembo, Dumizulu; Van Tyne, Daria; Voleman, Luboš; Walker,Dawn
Formato: Artículo (Article)
Lenguaje:Desconocido (Unknown)
Publicado: PLoS ONE; Vol. 7, No. 4 2012
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Acceso en línea:http://babel.banrepcultural.org/cdm/ref/collection/p17054coll23/id/544
id ir-p17054coll23-544
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spelling ir-p17054coll23-5442020-09-29 Live imaging of mitosomes and hydrogenosomes by halotag technology Sanín Peña, David Esteban; De Napoli, Maximiliano; Eshar, Shiri; Gualdrón López, Melisa; Hopp, Christine S.; Martincová, Eva; Najdrová, Vladimíra; Tembo, Dumizulu; Van Tyne, Daria; Voleman, Luboš; Walker,Dawn Hydrogenosomes and mitosomes represent remarkable mitochondrial adaptations in the anaerobic parasitic protists such as Trichomonas vaginalis and Giardia intestinalis, respectively. In order to provide a tool to study these organelles in the live cells, the HaloTag was fused to G. intestinalis IscU and T. vaginalis frataxin and expressed in the mitosomes and hydrogenosomes, respectively. The incubation of the parasites with the fluorescent Halo-ligand resulted in highly specific organellar labeling, allowing live imaging of the organelles. With the array of available ligands the HaloTag technology offers a new tool to study the dynamics of mitochondria-related compartments as well as other cellular components in these intriguing unicellular eukaryotes. Giardia intestinalis; Hidrogenosomas; Mitosomas; Protistas anaeróbicos; Trichomonas vaginalis Ciencias naturales y matemáticas; Ciencias naturales y matemáticas / Ciencias de la vida Biología; Tecnología; Tecnología / Ciencias médicas Medicina 2012 PLoS ONE; Vol. 7, No. 4 PDF Artículo ENG - Inglés Colfuturo © Derechos reservados del autor http://babel.banrepcultural.org/cdm/ref/collection/p17054coll23/id/544
institution Biblioteca Virtual Banco de la República - Colecciones digitales
collection Custom
language Desconocido (Unknown)
topic Giardia intestinalis; Hidrogenosomas; Mitosomas; Protistas anaeróbicos; Trichomonas vaginalis
Ciencias naturales y matemáticas; Ciencias naturales y matemáticas / Ciencias de la vida Biología; Tecnología; Tecnología / Ciencias médicas Medicina
spellingShingle Giardia intestinalis; Hidrogenosomas; Mitosomas; Protistas anaeróbicos; Trichomonas vaginalis
Ciencias naturales y matemáticas; Ciencias naturales y matemáticas / Ciencias de la vida Biología; Tecnología; Tecnología / Ciencias médicas Medicina
Sanín Peña, David Esteban; De Napoli, Maximiliano; Eshar, Shiri; Gualdrón López, Melisa; Hopp, Christine S.; Martincová, Eva; Najdrová, Vladimíra; Tembo, Dumizulu; Van Tyne, Daria; Voleman, Luboš; Walker,Dawn
Live imaging of mitosomes and hydrogenosomes by halotag technology
description Hydrogenosomes and mitosomes represent remarkable mitochondrial adaptations in the anaerobic parasitic protists such as Trichomonas vaginalis and Giardia intestinalis, respectively. In order to provide a tool to study these organelles in the live cells, the HaloTag was fused to G. intestinalis IscU and T. vaginalis frataxin and expressed in the mitosomes and hydrogenosomes, respectively. The incubation of the parasites with the fluorescent Halo-ligand resulted in highly specific organellar labeling, allowing live imaging of the organelles. With the array of available ligands the HaloTag technology offers a new tool to study the dynamics of mitochondria-related compartments as well as other cellular components in these intriguing unicellular eukaryotes.
format Artículo (Article)
author Sanín Peña, David Esteban; De Napoli, Maximiliano; Eshar, Shiri; Gualdrón López, Melisa; Hopp, Christine S.; Martincová, Eva; Najdrová, Vladimíra; Tembo, Dumizulu; Van Tyne, Daria; Voleman, Luboš; Walker,Dawn
author_facet Sanín Peña, David Esteban; De Napoli, Maximiliano; Eshar, Shiri; Gualdrón López, Melisa; Hopp, Christine S.; Martincová, Eva; Najdrová, Vladimíra; Tembo, Dumizulu; Van Tyne, Daria; Voleman, Luboš; Walker,Dawn
author_sort Sanín Peña, David Esteban; De Napoli, Maximiliano; Eshar, Shiri; Gualdrón López, Melisa; Hopp, Christine S.; Martincová, Eva; Najdrová, Vladimíra; Tembo, Dumizulu; Van Tyne, Daria; Voleman, Luboš; Walker,Dawn
title Live imaging of mitosomes and hydrogenosomes by halotag technology
title_short Live imaging of mitosomes and hydrogenosomes by halotag technology
title_full Live imaging of mitosomes and hydrogenosomes by halotag technology
title_fullStr Live imaging of mitosomes and hydrogenosomes by halotag technology
title_full_unstemmed Live imaging of mitosomes and hydrogenosomes by halotag technology
title_sort live imaging of mitosomes and hydrogenosomes by halotag technology
publisher PLoS ONE; Vol. 7, No. 4
publishDate 2012
url http://babel.banrepcultural.org/cdm/ref/collection/p17054coll23/id/544
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score 12,131701