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Intestinal expression of pro-infammatory cytokines induced by oral intake of lipopolysaccharide (LPS) from E. coli in weaned pigs

Background: Treatments with lipopolysaccharide (LPS) from E. coli are an accepted way of inducing inflammation in immunological studies since they have the ability to activate a coordinate series of signs through the synthesis of pro-inflammatory cytokines such as interleukin 8 (IL-8), IL-18 and tum...

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Autor Principal: Sanín Peña, David Esteban; Agudelo T., Jorge; Forero D., Jorge; López Herrera, Albeiro; Muskus L., Carlos; Parra S., Jaime
Formato: Artículo (Article)
Lenguaje:Desconocido (Unknown)
Publicado: Revista Colombiana de Ciencias Pecuarias; Vol. 26, No. 2 2013
Materias:
Gut immunology; Intestinal villi, Pig weaning, RT-PCR, TNF-?.
Ciencias naturales y matemáticas; Ciencias naturales y matemáticas / Ciencias de la vida Biología
Acceso en línea:http://babel.banrepcultural.org/cdm/ref/collection/p17054coll23/id/540
id ir-p17054coll23-540
recordtype dspace
spelling ir-p17054coll23-5402020-09-29 Intestinal expression of pro-infammatory cytokines induced by oral intake of lipopolysaccharide (LPS) from E. coli in weaned pigs Sanín Peña, David Esteban; Agudelo T., Jorge; Forero D., Jorge; López Herrera, Albeiro; Muskus L., Carlos; Parra S., Jaime Background: Treatments with lipopolysaccharide (LPS) from E. coli are an accepted way of inducing inflammation in immunological studies since they have the ability to activate a coordinate series of signs through the synthesis of pro-inflammatory cytokines such as interleukin 8 (IL-8), IL-18 and tumor necrosis factor alpha (TNF-?), which can cause significant changes in intestinal structure and functionality. Objective: the aim of this study was to evaluate the effect of adding LPS of E. coli on pro-inflammatory cytokine gene expression IL-8, IL-18 and TNF-? in early-weaned pigs. Methods:fieldwork was conducted at Centro San Pablo, belonging to the Universidad Nacional de Colombia with 32 pigs at 21 days of age, with 6.5 ± 0.5 kg of weight. Animals were fed with a basal diet supplemented with two levels of inclusion of LPS of E.coli serotype 0111:B4 (0 to 0.3 ?g/mg of food). Pigs were slaughtered in stages on days 1, 5, 7 and 10 postweaning and complete extraction of small intestine was made. Gene expression was evaluated by qPCR. Blocks at random in a factorial arrangement 2 x 4 were used as statistical design. Results: the basal diet (without addition of LPS) presented increase in mRNA expression (p < 0.01) of all the cytokines in jejunum for each post-weaning day, which suggests an inflammatory response and extensive tissue damage in pigs after early weaning. In diet 1 (with consumption of 0.3 ?g LPS / mg diet) cytokines TNF-?, IL-8 and IL-18 showed a significant increase in their levels of expression (p < 0.01). All cytokines presented significant increase (p < 0.01) in jejunum for each post-weaning day. Conclusion: The increase observed in the expression of TNF-? can be involved in the development of post-weaning diarrhea. Gut immunology; Intestinal villi, Pig weaning, RT-PCR, TNF-?. Ciencias naturales y matemáticas; Ciencias naturales y matemáticas / Ciencias de la vida Biología 2013 Revista Colombiana de Ciencias Pecuarias; Vol. 26, No. 2 PDF Artículo ENG - Inglés Medellín (Colombia) Colfuturo © Derechos reservados del autor http://babel.banrepcultural.org/cdm/ref/collection/p17054coll23/id/540
institution Biblioteca Virtual Banco de la República - Colecciones digitales
collection Custom
language Desconocido (Unknown)
topic Gut immunology; Intestinal villi, Pig weaning, RT-PCR, TNF-?.
Ciencias naturales y matemáticas; Ciencias naturales y matemáticas / Ciencias de la vida Biología
spellingShingle Gut immunology; Intestinal villi, Pig weaning, RT-PCR, TNF-?.
Ciencias naturales y matemáticas; Ciencias naturales y matemáticas / Ciencias de la vida Biología
Sanín Peña, David Esteban; Agudelo T., Jorge; Forero D., Jorge; López Herrera, Albeiro; Muskus L., Carlos; Parra S., Jaime
Intestinal expression of pro-infammatory cytokines induced by oral intake of lipopolysaccharide (LPS) from E. coli in weaned pigs
description Background: Treatments with lipopolysaccharide (LPS) from E. coli are an accepted way of inducing inflammation in immunological studies since they have the ability to activate a coordinate series of signs through the synthesis of pro-inflammatory cytokines such as interleukin 8 (IL-8), IL-18 and tumor necrosis factor alpha (TNF-?), which can cause significant changes in intestinal structure and functionality. Objective: the aim of this study was to evaluate the effect of adding LPS of E. coli on pro-inflammatory cytokine gene expression IL-8, IL-18 and TNF-? in early-weaned pigs. Methods:fieldwork was conducted at Centro San Pablo, belonging to the Universidad Nacional de Colombia with 32 pigs at 21 days of age, with 6.5 ± 0.5 kg of weight. Animals were fed with a basal diet supplemented with two levels of inclusion of LPS of E.coli serotype 0111:B4 (0 to 0.3 ?g/mg of food). Pigs were slaughtered in stages on days 1, 5, 7 and 10 postweaning and complete extraction of small intestine was made. Gene expression was evaluated by qPCR. Blocks at random in a factorial arrangement 2 x 4 were used as statistical design. Results: the basal diet (without addition of LPS) presented increase in mRNA expression (p < 0.01) of all the cytokines in jejunum for each post-weaning day, which suggests an inflammatory response and extensive tissue damage in pigs after early weaning. In diet 1 (with consumption of 0.3 ?g LPS / mg diet) cytokines TNF-?, IL-8 and IL-18 showed a significant increase in their levels of expression (p < 0.01). All cytokines presented significant increase (p < 0.01) in jejunum for each post-weaning day. Conclusion: The increase observed in the expression of TNF-? can be involved in the development of post-weaning diarrhea.
format Artículo (Article)
author Sanín Peña, David Esteban; Agudelo T., Jorge; Forero D., Jorge; López Herrera, Albeiro; Muskus L., Carlos; Parra S., Jaime
author_facet Sanín Peña, David Esteban; Agudelo T., Jorge; Forero D., Jorge; López Herrera, Albeiro; Muskus L., Carlos; Parra S., Jaime
author_sort Sanín Peña, David Esteban; Agudelo T., Jorge; Forero D., Jorge; López Herrera, Albeiro; Muskus L., Carlos; Parra S., Jaime
title Intestinal expression of pro-infammatory cytokines induced by oral intake of lipopolysaccharide (LPS) from E. coli in weaned pigs
title_short Intestinal expression of pro-infammatory cytokines induced by oral intake of lipopolysaccharide (LPS) from E. coli in weaned pigs
title_full Intestinal expression of pro-infammatory cytokines induced by oral intake of lipopolysaccharide (LPS) from E. coli in weaned pigs
title_fullStr Intestinal expression of pro-infammatory cytokines induced by oral intake of lipopolysaccharide (LPS) from E. coli in weaned pigs
title_full_unstemmed Intestinal expression of pro-infammatory cytokines induced by oral intake of lipopolysaccharide (LPS) from E. coli in weaned pigs
title_sort intestinal expression of pro-infammatory cytokines induced by oral intake of lipopolysaccharide (lps) from e. coli in weaned pigs
publisher Revista Colombiana de Ciencias Pecuarias; Vol. 26, No. 2
publishDate 2013
url http://babel.banrepcultural.org/cdm/ref/collection/p17054coll23/id/540
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score 12,131701

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