The dynamic role of the conserved WhiA and MinD proteins in chromosome segregation, fatty acid metabolism and cell division in Bacillus subtilis / El rol dinámico de las proteínas conservadas WhiA y MinD en segregación cromosómica, metabolismo de ácidos grasos y división celular en Bacillus subtilis

This thesis presents several studies, using genetics, cell biology techniques, transcriptomics and metabolomics, to investigate the function of cell division proteins. The protein WhiA is present in most Gram-positive bacteria, binds to DNA and induces expression of cell division genes. B. subtilis...

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Autor Principal: Bohórquez Suárez, Laura Catalina
Formato: Trabajo de grado (Bachelor Thesis)
Lenguaje:Desconocido (Unknown)
Publicado: 2018
Materias:
Acceso en línea:http://babel.banrepcultural.org/cdm/ref/collection/p17054coll23/id/1077
id ir-p17054coll23-1077
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spelling ir-p17054coll23-10772020-09-28 The dynamic role of the conserved WhiA and MinD proteins in chromosome segregation, fatty acid metabolism and cell division in Bacillus subtilis / El rol dinámico de las proteínas conservadas WhiA y MinD en segregación cromosómica, metabolismo de ácidos grasos y división celular en Bacillus subtilis Bohórquez Suárez, Laura Catalina This thesis presents several studies, using genetics, cell biology techniques, transcriptomics and metabolomics, to investigate the function of cell division proteins. The protein WhiA is present in most Gram-positive bacteria, binds to DNA and induces expression of cell division genes. B. subtilis WhiA is not a transcription factor and its functional role is unknown. Here, it was found that the whiA mutants show an increased space between their nucleoids. The deletion of whiA is synthetic lethal when DNA replication and segregation regulators are absent. However, WhiA does not affect replication initiation. The whiA mutants are highly sensitive for DNA damaging agents. The results indicate that WhiA has two separate roles in cell division and chromosome segregation. The whiA gene is part of an operon and flanked by two metabolic genes, therefore, B. subtilis WhiA might play a role in carbon metabolism. A metabolomics approach was followed and it was found that WhiA does not participate in carbon catabolite regulation, but affects fatty acid composition of the membrane. The B. subtilis Min system form a static gradient decreasing towards midcell, and prevent formation of minicells by blocking FtsZ polymerization close to cell poles. Here, it was found that the dynamic cycling between monomer and dimer is essential for a MinD gradient, and that the interaction with MinJ requires dimerization. The monomeric and dimeric forms of MinD have comparable membrane affinities. The results show that increasing the MinD membrane affinity strongly reduces the formation of a gradient and affects interaction with MinC. B. subtilis; Fluorescence microscopy; Genetics; Transcriptomics and metabolomics Ciencias naturales y matemáticas; Ciencias naturales y matemáticas / Química y ciencias afines 2018 PDF Tesis ENG - Inglés Países Bajos Colfuturo © Derechos reservados del autor http://babel.banrepcultural.org/cdm/ref/collection/p17054coll23/id/1077
institution Biblioteca Virtual Banco de la República - Colecciones digitales
collection Custom
language Desconocido (Unknown)
topic B. subtilis; Fluorescence microscopy; Genetics; Transcriptomics and metabolomics
Ciencias naturales y matemáticas; Ciencias naturales y matemáticas / Química y ciencias afines
spellingShingle B. subtilis; Fluorescence microscopy; Genetics; Transcriptomics and metabolomics
Ciencias naturales y matemáticas; Ciencias naturales y matemáticas / Química y ciencias afines
Bohórquez Suárez, Laura Catalina
The dynamic role of the conserved WhiA and MinD proteins in chromosome segregation, fatty acid metabolism and cell division in Bacillus subtilis / El rol dinámico de las proteínas conservadas WhiA y MinD en segregación cromosómica, metabolismo de ácidos grasos y división celular en Bacillus subtilis
description This thesis presents several studies, using genetics, cell biology techniques, transcriptomics and metabolomics, to investigate the function of cell division proteins. The protein WhiA is present in most Gram-positive bacteria, binds to DNA and induces expression of cell division genes. B. subtilis WhiA is not a transcription factor and its functional role is unknown. Here, it was found that the whiA mutants show an increased space between their nucleoids. The deletion of whiA is synthetic lethal when DNA replication and segregation regulators are absent. However, WhiA does not affect replication initiation. The whiA mutants are highly sensitive for DNA damaging agents. The results indicate that WhiA has two separate roles in cell division and chromosome segregation. The whiA gene is part of an operon and flanked by two metabolic genes, therefore, B. subtilis WhiA might play a role in carbon metabolism. A metabolomics approach was followed and it was found that WhiA does not participate in carbon catabolite regulation, but affects fatty acid composition of the membrane. The B. subtilis Min system form a static gradient decreasing towards midcell, and prevent formation of minicells by blocking FtsZ polymerization close to cell poles. Here, it was found that the dynamic cycling between monomer and dimer is essential for a MinD gradient, and that the interaction with MinJ requires dimerization. The monomeric and dimeric forms of MinD have comparable membrane affinities. The results show that increasing the MinD membrane affinity strongly reduces the formation of a gradient and affects interaction with MinC.
format Trabajo de grado (Bachelor Thesis)
author Bohórquez Suárez, Laura Catalina
author_facet Bohórquez Suárez, Laura Catalina
author_sort Bohórquez Suárez, Laura Catalina
title The dynamic role of the conserved WhiA and MinD proteins in chromosome segregation, fatty acid metabolism and cell division in Bacillus subtilis / El rol dinámico de las proteínas conservadas WhiA y MinD en segregación cromosómica, metabolismo de ácidos grasos y división celular en Bacillus subtilis
title_short The dynamic role of the conserved WhiA and MinD proteins in chromosome segregation, fatty acid metabolism and cell division in Bacillus subtilis / El rol dinámico de las proteínas conservadas WhiA y MinD en segregación cromosómica, metabolismo de ácidos grasos y división celular en Bacillus subtilis
title_full The dynamic role of the conserved WhiA and MinD proteins in chromosome segregation, fatty acid metabolism and cell division in Bacillus subtilis / El rol dinámico de las proteínas conservadas WhiA y MinD en segregación cromosómica, metabolismo de ácidos grasos y división celular en Bacillus subtilis
title_fullStr The dynamic role of the conserved WhiA and MinD proteins in chromosome segregation, fatty acid metabolism and cell division in Bacillus subtilis / El rol dinámico de las proteínas conservadas WhiA y MinD en segregación cromosómica, metabolismo de ácidos grasos y división celular en Bacillus subtilis
title_full_unstemmed The dynamic role of the conserved WhiA and MinD proteins in chromosome segregation, fatty acid metabolism and cell division in Bacillus subtilis / El rol dinámico de las proteínas conservadas WhiA y MinD en segregación cromosómica, metabolismo de ácidos grasos y división celular en Bacillus subtilis
title_sort dynamic role of the conserved whia and mind proteins in chromosome segregation, fatty acid metabolism and cell division in bacillus subtilis / el rol dinámico de las proteínas conservadas whia y mind en segregación cromosómica, metabolismo de ácidos grasos y división celular en bacillus subtilis
publishDate 2018
url http://babel.banrepcultural.org/cdm/ref/collection/p17054coll23/id/1077
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score 12,131701