Citrus leprosis transmission by Brevipalpus yothersi mites through non citrus hosts

Citrus leprosis virus (C i LV) was detected in Colombia at the eastern plains in 2004; it is a threat the disease spreads to other regions of the country. The main vector is Brevipalpus yothersi Baker (formerly identified as Brevipalpus phoenicis). This research determined the viability of B. yother...

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Autores Principales: León M., Guillermo, Roy, Avijit, Choudhary, Nandlal, Brlansky, Ronald
Formato: Artículo (Article)
Lenguaje:Español (Spanish)
Publicado: Corporación Colombiana de Investigación Agropecuaria (Agrosavia) 2017
Acceso en línea:http://hdl.handle.net/20.500.12324/35250
id ir-20.500.12324-35250
recordtype dspace
institution Agrosavia
collection DSpace
language Español (Spanish)
description Citrus leprosis virus (C i LV) was detected in Colombia at the eastern plains in 2004; it is a threat the disease spreads to other regions of the country. The main vector is Brevipalpus yothersi Baker (formerly identified as Brevipalpus phoenicis). This research determined the viability of B. yothersi to transmit C i LV to citrus plants, after been hosted in non-citrus plants. To virus acquisition, mites spent three days on symptomatic orange (Citrus x sinensis) leaves positives to C i LV-C2; then mites were placed on six non-citrus plants (Dieffenbachia sp., Hibiscus rosa-sinensis,Codiaeum variegatum, Swinglea glutinosa, Sida acutaand Stachytarpheta cayennensis). A randomized design with 6 treatments and 4 replicates was carried out. After scheduled time in non-citrus plants, mites were three days relocated on C. x sinensis healthy plants. Leaves of receptor plants, were evaluated to the occurrence or absence of symptoms and collected for RT-PCR tests. B. yothersi mites were able to transmit the C i LV virus over 85 % of Valencia orange plants (Citrus x sinensis L.), after feeding from 2-20 days on non-citrus host plants. The first leprosis symptoms on C. x sinensis leaves was confirmed from 14 to 51 days after transmission. The present research work further established that C i LV-C2 is a persistently transmitted virus. The implement quarantine diagnostic measures to prevent spread of CiLV to disease-free zones is suggested.
format Artículo (Article)
author León M., Guillermo
Roy, Avijit
Choudhary, Nandlal
Brlansky, Ronald
Brlansky, Ronald
spellingShingle León M., Guillermo
Roy, Avijit
Choudhary, Nandlal
Brlansky, Ronald
Brlansky, Ronald
Citrus leprosis transmission by Brevipalpus yothersi mites through non citrus hosts
author_facet León M., Guillermo
Roy, Avijit
Choudhary, Nandlal
Brlansky, Ronald
Brlansky, Ronald
author_sort León M., Guillermo
title Citrus leprosis transmission by Brevipalpus yothersi mites through non citrus hosts
title_short Citrus leprosis transmission by Brevipalpus yothersi mites through non citrus hosts
title_full Citrus leprosis transmission by Brevipalpus yothersi mites through non citrus hosts
title_fullStr Citrus leprosis transmission by Brevipalpus yothersi mites through non citrus hosts
title_full_unstemmed Citrus leprosis transmission by Brevipalpus yothersi mites through non citrus hosts
title_sort citrus leprosis transmission by brevipalpus yothersi mites through non citrus hosts
publisher Corporación Colombiana de Investigación Agropecuaria (Agrosavia)
publishDate 2017
url http://hdl.handle.net/20.500.12324/35250
_version_ 1642273885988585472
spelling ir-20.500.12324-352502019-08-09T19:30:17Z Citrus leprosis transmission by Brevipalpus yothersi mites through non citrus hosts Transmisión de leprosis de los cítricos por ácaros Brevipalpus yothersi a través de hospederos no cítricos Transmissão do vírus da leprose dos citros por ácarosBrevipalpus yothersi por meio de hospedeiros não cítricos León M., Guillermo Roy, Avijit Choudhary, Nandlal Brlansky, Ronald Brlansky, Ronald Citrus leprosis virus (C i LV) was detected in Colombia at the eastern plains in 2004; it is a threat the disease spreads to other regions of the country. The main vector is Brevipalpus yothersi Baker (formerly identified as Brevipalpus phoenicis). This research determined the viability of B. yothersi to transmit C i LV to citrus plants, after been hosted in non-citrus plants. To virus acquisition, mites spent three days on symptomatic orange (Citrus x sinensis) leaves positives to C i LV-C2; then mites were placed on six non-citrus plants (Dieffenbachia sp., Hibiscus rosa-sinensis,Codiaeum variegatum, Swinglea glutinosa, Sida acutaand Stachytarpheta cayennensis). A randomized design with 6 treatments and 4 replicates was carried out. After scheduled time in non-citrus plants, mites were three days relocated on C. x sinensis healthy plants. Leaves of receptor plants, were evaluated to the occurrence or absence of symptoms and collected for RT-PCR tests. B. yothersi mites were able to transmit the C i LV virus over 85 % of Valencia orange plants (Citrus x sinensis L.), after feeding from 2-20 days on non-citrus host plants. The first leprosis symptoms on C. x sinensis leaves was confirmed from 14 to 51 days after transmission. The present research work further established that C i LV-C2 is a persistently transmitted virus. The implement quarantine diagnostic measures to prevent spread of CiLV to disease-free zones is suggested. El virus de la leprosis de los cítricos (CiLV) se detectó en los Llanos Orientales en 2004. Es una amenaza para la citricultura colombiana si logra extenderse hacia otras regiones del país. El principal vector es el ácaro Brevipalpus yothersi Baker (antes, Brevipalpus phoenicis). En esta investigación se determinó que B. yothersi puede transmitir CiLV a plantas cítricas luego de hospedarse en plantas no cítricas. Inicialmente se permitió la adquisición del virus por parte de los ácaros durante tres días sobre hojas sintomáticas de naranjos (Citrus x sinensis) positivos a CiLV-C2. Luego, los ácaros se ubicaron sobre seis plantas no cítricas (Dieffenbachia sp., Hibiscus rosa-sinensis, Codiaeum variegatum, Swinglea glutinosa, Sida acuta y Stachytarpheta cayennensis), de acuerdo con un diseño experimental completamente al azar con seis tratamientos y cuatro repeticiones. Cumplido el tiempo programado, los ácaros se reubicaron por tres días sobre plantas sanas de C. x sinensis. Las hojas de las plantas receptoras se evaluaron según la presencia de síntomas y se colectaron para pruebas RT-PCR. Los resultados muestran que B. yothersi transmite el CiLV con más de un 85 % de efectividad hacia plantas de C. x sinensis, luego de haber permanecido en cualquiera de los hospederos alternos. Esta investigación sirve de apoyo al desarrollo de medidas cuarentenarias de diagnóstico para evitar la dispersión del virus. O vírus da leprose dos citros (CiLV) foi detectado na planície oriental da Colômbia em 2004. É uma ameaça para a citricultura colombiana se conseguir se expandir a outras regiões do país. O principal vetor é o ácaro Brevipalpus yothersi Baker (antes, Brevipalpus phoenicis). Nesta pesquisa, foi determi-nado que B. yothersi pode transmitir CiLV a plantas cítricas após ser hospedado em plantas não cítricas. Inicialmente, permitiu-se a aquisição do vírus por parte dos ácaros durante três dias sobre folhas sintomáticas de laranjeiras (Citrus x sinensis) positivas a CILV-C2. Em seguida, os ácaros se localizaram sobre Dieffenbachia sp., Hibiscus rosa-sinensis, Codiaeum variegatum, Swinglea glutinosa, Sida acuta e Stachytarpheta cayennensis, de acordo com um desenho experimental completamente ao acaso com seis tratamentos e quatro repetições. Cumprido o tempo programado, os ácaros se reposicionaram por três dias sobre plantas sãs de C. x sinensis. As folhas das plantas receptoras foram avaliadas segundo a presença de sintomas e foram coletadas para testes RT-P C R. Os resultados mostram que B. yothersitransmite o CiLV com mais de 85 % de efetividade a plantas de C. x sinensis, após ter permanecido em qualquer das hospedeiras alternas. Esta pesquisa serve de apoio para o desenvolvimento de medidas de quarentena de diagnóstico para evitar a propagação do vírus. 2017-05-08 2019-08-09T19:30:17Z 2019-08-09T19:30:17Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://revistacta.agrosavia.co/index.php/revista/article/view/633 10.21930/rcta.vol18_num2_art:633 http://hdl.handle.net/20.500.12324/35250 spa http://revistacta.agrosavia.co/index.php/revista/article/view/633/451 application/pdf Corporación Colombiana de Investigación Agropecuaria (Agrosavia) Ciencia y Tecnología Agropecuaria; Vol 18 No 2 (2017); 307-319 Ciencia y Tecnología Agropecuaria; Vol. 18 Núm. 2 (2017); 307-319 revista Corpoica Ciência e Tecnologia Agropecuária; v. 18 n. 2 (2017); 307-319 2500-5308 0122-8706 10.21930/rcta.vol18-num2
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