| Sumario: | Nitrogen loss, from the nitrification of nitrogen fertilizer, creates pollution through nitrous oxide emissions and nitrate leaching. The reports on Brachiaria as a biological nitrification inhibitor were evaluated to determine nitrate losses of Brachiaria humidicola CIAT 679 (indicator plant for biological nitrification inhibition) and Panicum maximum cv. tanzania (non-inhibiting plant). The incubated soil technique was used for the production of nitrate and for losses of nitrate, ion exchange PRSTM Probes resins were used. The nitrogen treatments were 0, 150 and 300 kg ha-1 per year, the resins were installed at three depths in the soil, laboratory analysis was performed using ultraviolet-visible spectroscopy with a wavelength of 410 nm for nitrate. B. humidicola reduced outputs of nitrate in the soil and the nitrogen doses did not generate variations in production, which showed an effect on the inhibition of nitrification. Nitrate losses were reduced after 18 months in B. humidicola, and P. maximum can avoid nitrate losses with its ability to take nitrogen from the soil in an ammonia form, but does not reduce nitrate production, and does not inhibit nitrification. In cattle pasture soils, B. humidicola can reduce nitrate production and loss. P. maximum with its ability and good response to nitrogenated fertilization could have reduced losses, but failed to reduce nitrate production.
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