Silver nanostructured platforms for detecting Escherichia coli through Raman scattering spectroscopy “Proof of concept”

Surface enhanced Raman spectroscopy (SERS) of nanostructured materials is a powerful technique that allows to reach ultrahigh levels of detection of several analytes. In the present study it was possible to identify the bacteria E. coli by using a novel nanostructured platform based on silver-capped...

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Autores Principales: Castillo-León, John Jairo, Rincón-Orozco, Bladimiro, Cabanzo-Hernández, Rafael
Formato: Artículo (Article)
Lenguaje:Español (Spanish)
Publicado: Universidad Santo Tomás. Seccional Bucaramanga 2017
Acceso en línea:http://hdl.handle.net/11634/36179
id ir-11634-36179
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spelling ir-11634-361792021-09-24T13:17:49Z Silver nanostructured platforms for detecting Escherichia coli through Raman scattering spectroscopy “Proof of concept” Plataformas nanoestructuradas de plata para identificación cualitativa de Escherichia coli mediante espectroscopia Raman intensificada por efecto de superficie “prueba de concepto del sistema” Castillo-León, John Jairo Rincón-Orozco, Bladimiro Cabanzo-Hernández, Rafael Surface enhanced Raman spectroscopy (SERS) of nanostructured materials is a powerful technique that allows to reach ultrahigh levels of detection of several analytes. In the present study it was possible to identify the bacteria E. coli by using a novel nanostructured platform based on silver-capped nanopillars (AgNP) and SERS technique. AgNPs were fabricated by ion reactive etching and deposition of silver layers. The bacteria culture were prepared in Luria-Bertani (LB) medium at 37° by 4 hours. 5 μL of the bacteria were deposited on top of the surface of AgNPs and let it dry for 30 minutes. Subsequently the AgNP-bacteria system was analysed by Raman spectroscopy. A typical band of E. coli at 731 cm-1 was identified and this Raman vibration was used as a marker peak to detect the bacteria. Finally the novel AgNP could be used as a potential biosensor to detect nosocomial bacteria in intra-hospital environments. La espectroscopia Raman intensificada por efecto de superficie (SERS) de materiales nanoestructurados es una técnica ultrasensible que permite alcanzar niveles de detección extremadamente bajos en la determinación de diferentes analitos. En este estudio se logró identificar cualitativamente la bacteria E. coli, utilizando plataformas nanoestructuradas de plata, mediante la técnica SERS. Las plataformas nanoestructuradas de plata (AgNP) fueron fabricadas por la técnica de grabado mediante iones reactivos con posterior deposición de una capa metálica de plata. Las bacterias fueron preparadas en medio Luria-Bertani (LB) a 37°C por 4 horas. De este cultivo de bacterias 5 uL fueron depositados sobre la superficie de las AgNP, dejándose secar por 30 min. Posteriormente, el sistema AgNP-bacteria fue analizado mediante espectroscopia Raman. La identificación de la banda Raman a 731 nm típica de la E. coli sobre las AgNP permitió identificar cualitativamente la bacteria, lo que brinda la posibilidad de utilizar las plataformas como potenciales biosensores ultrasensibles de bacterias nosocomiales en ambientes intrahospitalarios. 2017-07-01 2021-09-24T13:17:49Z 2021-09-24T13:17:49Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://revistas.ustabuca.edu.co/index.php/ITECKNE/article/view/1771 10.15332/iteckne.v14i2.1771 http://hdl.handle.net/11634/36179 spa http://revistas.ustabuca.edu.co/index.php/ITECKNE/article/view/1771/1359 Copyright (c) 2018 ITECKNE application/pdf Universidad Santo Tomás. Seccional Bucaramanga ITECKNE; Vol 14 No 2 (2017); 164-169 ITECKNE; Vol 14 No 2 (2017); 164-169 2339-3483 1692-1798
institution Universidad Santo Tomas
collection DSpace
language Español (Spanish)
description Surface enhanced Raman spectroscopy (SERS) of nanostructured materials is a powerful technique that allows to reach ultrahigh levels of detection of several analytes. In the present study it was possible to identify the bacteria E. coli by using a novel nanostructured platform based on silver-capped nanopillars (AgNP) and SERS technique. AgNPs were fabricated by ion reactive etching and deposition of silver layers. The bacteria culture were prepared in Luria-Bertani (LB) medium at 37° by 4 hours. 5 μL of the bacteria were deposited on top of the surface of AgNPs and let it dry for 30 minutes. Subsequently the AgNP-bacteria system was analysed by Raman spectroscopy. A typical band of E. coli at 731 cm-1 was identified and this Raman vibration was used as a marker peak to detect the bacteria. Finally the novel AgNP could be used as a potential biosensor to detect nosocomial bacteria in intra-hospital environments.
format Artículo (Article)
author Castillo-León, John Jairo
Rincón-Orozco, Bladimiro
Cabanzo-Hernández, Rafael
spellingShingle Castillo-León, John Jairo
Rincón-Orozco, Bladimiro
Cabanzo-Hernández, Rafael
Silver nanostructured platforms for detecting Escherichia coli through Raman scattering spectroscopy “Proof of concept”
author_facet Castillo-León, John Jairo
Rincón-Orozco, Bladimiro
Cabanzo-Hernández, Rafael
author_sort Castillo-León, John Jairo
title Silver nanostructured platforms for detecting Escherichia coli through Raman scattering spectroscopy “Proof of concept”
title_short Silver nanostructured platforms for detecting Escherichia coli through Raman scattering spectroscopy “Proof of concept”
title_full Silver nanostructured platforms for detecting Escherichia coli through Raman scattering spectroscopy “Proof of concept”
title_fullStr Silver nanostructured platforms for detecting Escherichia coli through Raman scattering spectroscopy “Proof of concept”
title_full_unstemmed Silver nanostructured platforms for detecting Escherichia coli through Raman scattering spectroscopy “Proof of concept”
title_sort silver nanostructured platforms for detecting escherichia coli through raman scattering spectroscopy “proof of concept”
publisher Universidad Santo Tomás. Seccional Bucaramanga
publishDate 2017
url http://hdl.handle.net/11634/36179
_version_ 1712105936193060864
score 12,131701