| Sumario: | Alpha and beta diversity and the distribution of substrates of rheophilous bryophytes in three streams were determinate (Salto del Sereno, Pajarito and Paraíso localities), located in Montes de María (Sucre, Colombia). Two transects of 50 x 2m were conducted at each sampling site during the rainy season. The class Bryopsida presented 22 species and Hepaticopsida presented 9. The families of mosses with the largest number of species were Bryaceae (3 species), Neckeraceae (2), Pottiaceae (2). In liverworts, Lejeuneaceae (6), and the richest genera were Fissidens (5 species), Fabronia (2), Lejeunea (3) and Mastigolejeunea (2). The type of substrate with increased incidence of bryophytes was the epilithic (29 species), followed by epiphyte-corticolous (16) and terrestrial (13). The 31 species registered here, are new records for the department, 24 for the Caribbean region and one for Colombia (Hyophiladelphus agrarius).
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