A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies
Immunity to severe malaria is the first level of immunity acquired to Plasmodium falciparum. Antibodies to the variant antigen PfEMP1 (P. falciparum erythrocyte membrane protein 1) present at the surface of the parasitized red blood cell (pRBC) confer protection by blocking microvascular sequestrati...
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Universidad del Rosario
2013
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Acceso en línea: | http://repository.urosario.edu.co/handle/10336/8765 https://doi.org/10.1371/journal.pone.0052679 |
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ir-10336-87652022-08-31T17:44:56Z A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies Blomqvist, Karin Albrecht, Letusa Quintana, Maria del Pilar Angeletti, Davide Joannin, Nicolas Chêne, Arnaud Moll, Kirsten Wahlgren, Mats Erythrocyte membrane protein 1 protozoan Protein antibody Molecular Preschool Strain transcending antibody Unclassified drug Amino acid sequence Antigen antibody reaction Antigen binding Child Disease severity Erythrocyte Flow cytometry Immunofluorescence test In vitro study Plasmodium falciparum Preschool child Protein binding Protein domain Protein motif Amino acid motifs Erythrocytes Immunoglobulin g Molecular sequence data Plasmodium falciparum Plasmodium falciparum Malaria Inmunología PfEMP1 Plasmodium Immunity to severe malaria is the first level of immunity acquired to Plasmodium falciparum. Antibodies to the variant antigen PfEMP1 (P. falciparum erythrocyte membrane protein 1) present at the surface of the parasitized red blood cell (pRBC) confer protection by blocking microvascular sequestration. Here we have generated antibodies to peptide sequences of subdomain 2 of PfEMP1-DBL1a previously identified to be associated with severe or mild malaria. A set of sera generated to the amino acid sequence KLQTLTLHQVREYWWALNRKEVWKA, containing the motif ALNRKE, stained the live pRBC. 50% of parasites tested (7/14) were positive both in flow cytometry and immunofluorescence assays with live pRBCs including both laboratory strains and in vitro adapted clinical isolates. Antibodies that reacted selectively with the sequence REYWWALNRKEVWKA in a 15-mer peptide array of DBL1a-domains were also found to react with the pRBC surface. By utilizing a peptide array to map the binding properties of the elicited anti-DBL1a antibodies, the amino acids WxxNRx were found essential for antibody binding. Complementary experiments using 135 degenerate RDSM peptide sequences obtained from 93 Ugandan patient-isolates showed that antibody binding occurred when the amino acids WxLNRKE/D were present in the peptide. The data suggests that the ALNRKE sequence motif, associated with severe malaria, induces strain-transcending antibodies that react with the pRBC surface 2013-01-15 2014-08-11T14:48:06Z info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion 1932-6203 http://repository.urosario.edu.co/handle/10336/8765 https://doi.org/10.1371/journal.pone.0052679 eng info:eu-repo/semantics/openAccess application/pdf Universidad del Rosario instname:Universidad del Rosario |
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EdocUR - Universidad del Rosario |
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language |
Inglés (English) |
topic |
Erythrocyte membrane protein 1 protozoan Protein antibody Molecular Preschool Strain transcending antibody Unclassified drug Amino acid sequence Antigen antibody reaction Antigen binding Child Disease severity Erythrocyte Flow cytometry Immunofluorescence test In vitro study Plasmodium falciparum Preschool child Protein binding Protein domain Protein motif Amino acid motifs Erythrocytes Immunoglobulin g Molecular sequence data Plasmodium falciparum Plasmodium falciparum Malaria Inmunología PfEMP1 Plasmodium |
spellingShingle |
Erythrocyte membrane protein 1 protozoan Protein antibody Molecular Preschool Strain transcending antibody Unclassified drug Amino acid sequence Antigen antibody reaction Antigen binding Child Disease severity Erythrocyte Flow cytometry Immunofluorescence test In vitro study Plasmodium falciparum Preschool child Protein binding Protein domain Protein motif Amino acid motifs Erythrocytes Immunoglobulin g Molecular sequence data Plasmodium falciparum Plasmodium falciparum Malaria Inmunología PfEMP1 Plasmodium Blomqvist, Karin Albrecht, Letusa Quintana, Maria del Pilar Angeletti, Davide Joannin, Nicolas Chêne, Arnaud Moll, Kirsten Wahlgren, Mats A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies |
description |
Immunity to severe malaria is the first level of immunity acquired to Plasmodium falciparum. Antibodies to the variant antigen PfEMP1 (P. falciparum erythrocyte membrane protein 1) present at the surface of the parasitized red blood cell (pRBC) confer protection by blocking microvascular sequestration. Here we have generated antibodies to peptide sequences of subdomain 2 of PfEMP1-DBL1a previously identified to be associated with severe or mild malaria. A set of sera
generated to the amino acid sequence KLQTLTLHQVREYWWALNRKEVWKA, containing the motif ALNRKE, stained the live pRBC. 50% of parasites tested (7/14) were positive both in flow cytometry and immunofluorescence assays with live pRBCs including both laboratory strains and in vitro adapted clinical isolates. Antibodies that reacted selectively with the sequence REYWWALNRKEVWKA in a 15-mer peptide array of DBL1a-domains were also found to react with the pRBC surface. By utilizing a peptide array to map the binding properties of the elicited anti-DBL1a antibodies, the amino acids WxxNRx were found essential for antibody binding. Complementary experiments using 135 degenerate RDSM peptide sequences obtained from 93 Ugandan patient-isolates showed that antibody binding occurred when the amino acids WxLNRKE/D were present in the peptide. The data suggests that the ALNRKE sequence motif, associated with severe malaria, induces strain-transcending antibodies that react with the pRBC surface |
format |
Artículo (Article) |
author |
Blomqvist, Karin Albrecht, Letusa Quintana, Maria del Pilar Angeletti, Davide Joannin, Nicolas Chêne, Arnaud Moll, Kirsten Wahlgren, Mats |
author_facet |
Blomqvist, Karin Albrecht, Letusa Quintana, Maria del Pilar Angeletti, Davide Joannin, Nicolas Chêne, Arnaud Moll, Kirsten Wahlgren, Mats |
author_sort |
Blomqvist, Karin |
title |
A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies |
title_short |
A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies |
title_full |
A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies |
title_fullStr |
A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies |
title_full_unstemmed |
A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies |
title_sort |
sequence in subdomain 2 of dbl1a of plasmodium falciparum erythrocyte membrane protein 1 induces strain transcending antibodies |
publisher |
Universidad del Rosario |
publishDate |
2013 |
url |
http://repository.urosario.edu.co/handle/10336/8765 https://doi.org/10.1371/journal.pone.0052679 |
_version_ |
1743430988723126272 |
score |
12,131701 |