A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies

Immunity to severe malaria is the first level of immunity acquired to Plasmodium falciparum. Antibodies to the variant antigen PfEMP1 (P. falciparum erythrocyte membrane protein 1) present at the surface of the parasitized red blood cell (pRBC) confer protection by blocking microvascular sequestrati...

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Autores Principales: Blomqvist, Karin, Albrecht, Letusa, Quintana, Maria del Pilar, Angeletti, Davide, Joannin, Nicolas, Chêne, Arnaud, Moll, Kirsten, Wahlgren, Mats
Formato: Artículo (Article)
Lenguaje:Inglés (English)
Publicado: Universidad del Rosario 2013
Materias:
Acceso en línea:http://repository.urosario.edu.co/handle/10336/8765
https://doi.org/10.1371/journal.pone.0052679
id ir-10336-8765
recordtype dspace
spelling ir-10336-87652022-08-31T17:44:56Z A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies Blomqvist, Karin Albrecht, Letusa Quintana, Maria del Pilar Angeletti, Davide Joannin, Nicolas Chêne, Arnaud Moll, Kirsten Wahlgren, Mats Erythrocyte membrane protein 1 protozoan Protein antibody Molecular Preschool Strain transcending antibody Unclassified drug Amino acid sequence Antigen antibody reaction Antigen binding Child Disease severity Erythrocyte Flow cytometry Immunofluorescence test In vitro study Plasmodium falciparum Preschool child Protein binding Protein domain Protein motif Amino acid motifs Erythrocytes Immunoglobulin g Molecular sequence data Plasmodium falciparum Plasmodium falciparum Malaria Inmunología PfEMP1 Plasmodium Immunity to severe malaria is the first level of immunity acquired to Plasmodium falciparum. Antibodies to the variant antigen PfEMP1 (P. falciparum erythrocyte membrane protein 1) present at the surface of the parasitized red blood cell (pRBC) confer protection by blocking microvascular sequestration. Here we have generated antibodies to peptide sequences of subdomain 2 of PfEMP1-DBL1a previously identified to be associated with severe or mild malaria. A set of sera generated to the amino acid sequence KLQTLTLHQVREYWWALNRKEVWKA, containing the motif ALNRKE, stained the live pRBC. 50% of parasites tested (7/14) were positive both in flow cytometry and immunofluorescence assays with live pRBCs including both laboratory strains and in vitro adapted clinical isolates. Antibodies that reacted selectively with the sequence REYWWALNRKEVWKA in a 15-mer peptide array of DBL1a-domains were also found to react with the pRBC surface. By utilizing a peptide array to map the binding properties of the elicited anti-DBL1a antibodies, the amino acids WxxNRx were found essential for antibody binding. Complementary experiments using 135 degenerate RDSM peptide sequences obtained from 93 Ugandan patient-isolates showed that antibody binding occurred when the amino acids WxLNRKE/D were present in the peptide. The data suggests that the ALNRKE sequence motif, associated with severe malaria, induces strain-transcending antibodies that react with the pRBC surface 2013-01-15 2014-08-11T14:48:06Z info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion 1932-6203 http://repository.urosario.edu.co/handle/10336/8765 https://doi.org/10.1371/journal.pone.0052679 eng info:eu-repo/semantics/openAccess application/pdf Universidad del Rosario instname:Universidad del Rosario
institution EdocUR - Universidad del Rosario
collection DSpace
language Inglés (English)
topic Erythrocyte membrane protein 1
protozoan
Protein antibody
Molecular
Preschool
Strain transcending antibody
Unclassified drug
Amino acid sequence
Antigen antibody reaction
Antigen binding
Child
Disease severity
Erythrocyte
Flow cytometry
Immunofluorescence test
In vitro study
Plasmodium falciparum
Preschool child
Protein binding
Protein domain
Protein motif
Amino acid motifs
Erythrocytes
Immunoglobulin g
Molecular sequence data
Plasmodium falciparum
Plasmodium falciparum
Malaria
Inmunología
PfEMP1
Plasmodium
spellingShingle Erythrocyte membrane protein 1
protozoan
Protein antibody
Molecular
Preschool
Strain transcending antibody
Unclassified drug
Amino acid sequence
Antigen antibody reaction
Antigen binding
Child
Disease severity
Erythrocyte
Flow cytometry
Immunofluorescence test
In vitro study
Plasmodium falciparum
Preschool child
Protein binding
Protein domain
Protein motif
Amino acid motifs
Erythrocytes
Immunoglobulin g
Molecular sequence data
Plasmodium falciparum
Plasmodium falciparum
Malaria
Inmunología
PfEMP1
Plasmodium
Blomqvist, Karin
Albrecht, Letusa
Quintana, Maria del Pilar
Angeletti, Davide
Joannin, Nicolas
Chêne, Arnaud
Moll, Kirsten
Wahlgren, Mats
A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies
description Immunity to severe malaria is the first level of immunity acquired to Plasmodium falciparum. Antibodies to the variant antigen PfEMP1 (P. falciparum erythrocyte membrane protein 1) present at the surface of the parasitized red blood cell (pRBC) confer protection by blocking microvascular sequestration. Here we have generated antibodies to peptide sequences of subdomain 2 of PfEMP1-DBL1a previously identified to be associated with severe or mild malaria. A set of sera generated to the amino acid sequence KLQTLTLHQVREYWWALNRKEVWKA, containing the motif ALNRKE, stained the live pRBC. 50% of parasites tested (7/14) were positive both in flow cytometry and immunofluorescence assays with live pRBCs including both laboratory strains and in vitro adapted clinical isolates. Antibodies that reacted selectively with the sequence REYWWALNRKEVWKA in a 15-mer peptide array of DBL1a-domains were also found to react with the pRBC surface. By utilizing a peptide array to map the binding properties of the elicited anti-DBL1a antibodies, the amino acids WxxNRx were found essential for antibody binding. Complementary experiments using 135 degenerate RDSM peptide sequences obtained from 93 Ugandan patient-isolates showed that antibody binding occurred when the amino acids WxLNRKE/D were present in the peptide. The data suggests that the ALNRKE sequence motif, associated with severe malaria, induces strain-transcending antibodies that react with the pRBC surface
format Artículo (Article)
author Blomqvist, Karin
Albrecht, Letusa
Quintana, Maria del Pilar
Angeletti, Davide
Joannin, Nicolas
Chêne, Arnaud
Moll, Kirsten
Wahlgren, Mats
author_facet Blomqvist, Karin
Albrecht, Letusa
Quintana, Maria del Pilar
Angeletti, Davide
Joannin, Nicolas
Chêne, Arnaud
Moll, Kirsten
Wahlgren, Mats
author_sort Blomqvist, Karin
title A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies
title_short A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies
title_full A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies
title_fullStr A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies
title_full_unstemmed A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies
title_sort sequence in subdomain 2 of dbl1a of plasmodium falciparum erythrocyte membrane protein 1 induces strain transcending antibodies
publisher Universidad del Rosario
publishDate 2013
url http://repository.urosario.edu.co/handle/10336/8765
https://doi.org/10.1371/journal.pone.0052679
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score 12,131701