Multilocus PCR-RFLP profiling in Trypanosoma cruzi I highlights an intraspecific genetic variation pattern

Chagas disease represents a serious problem in public health. This zoonotic pathology is caused by the kinetoplastid Trypanosoma cruzi which displays a high genetic diversity falling into six Discrete Typing Units (TcI–TcVI). In Colombia, the prevalent DTU is TcI with findings of TcII, TcIII and TcI...

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Autores Principales: Ramírez, Juan David, Duque, María Clara, Montilla, Marleny, Cucunubá, Zulma M., Guhl, Felipe
Formato: Artículo (Article)
Lenguaje:Inglés (English)
Publicado: Elsevier 2012
Materias:
Acceso en línea:https://repository.urosario.edu.co/handle/10336/28161
https://doi.org/10.1016/j.meegid.2012.06.018
id ir-10336-28161
recordtype dspace
spelling ir-10336-281612020-08-19T14:46:11Z Multilocus PCR-RFLP profiling in Trypanosoma cruzi I highlights an intraspecific genetic variation pattern El perfil multilocus PCR-RFLP en Trypanosoma cruzi I destaca un patrón de variación genética intraespecífica Ramírez, Juan David Duque, María Clara Montilla, Marleny Cucunubá, Zulma M. Guhl, Felipe Discrete typing units Chagas disease PCR-RFLP Trypanosoma cruzi I Genetic diversity Genotypes Chagas disease represents a serious problem in public health. This zoonotic pathology is caused by the kinetoplastid Trypanosoma cruzi which displays a high genetic diversity falling into six Discrete Typing Units (TcI–TcVI). In Colombia, the prevalent DTU is TcI with findings of TcII, TcIII and TcIV in low proportions. The aim of this work was to observe the genetic variability within TcI using a multilocus PCR-RFLP strategy. We analyzed 70 single-celled clones from triatomines, reservoirs and humans that were amplified and restricted via ten PCR-RFLPs targets across TcI genome, the restriction fragments were used to construct phylograms according to calculated genetic distances. We obtained five polymorphic targets (1f8, HSP60, HSP70, SAPA and H1) and the consensus tree constructed according to these regions allowed us to observe two well-defined groups with close association to the transmission cycles (domestic/peridomestic and sylvatic) of Chagas disease in Colombia. Our findings allowed us to corroborate the previous reported genotypes based on the intergenic region of mini-exon gene. More studies examining the genetic diversity among T. cruzi I populations must be conducted in order to obtain a better understanding in regions where this DTU is endemic. 2012-12 2020-08-19T14:46:11Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion ISSN: 1567-1348 EISSN: 1567-7257 https://repository.urosario.edu.co/handle/10336/28161 https://doi.org/10.1016/j.meegid.2012.06.018 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Infection, Genetics and Evolution
institution EdocUR - Universidad del Rosario
collection DSpace
language Inglés (English)
topic Discrete typing units
Chagas disease
PCR-RFLP
Trypanosoma cruzi I
Genetic diversity
Genotypes
spellingShingle Discrete typing units
Chagas disease
PCR-RFLP
Trypanosoma cruzi I
Genetic diversity
Genotypes
Ramírez, Juan David
Duque, María Clara
Montilla, Marleny
Cucunubá, Zulma M.
Guhl, Felipe
Multilocus PCR-RFLP profiling in Trypanosoma cruzi I highlights an intraspecific genetic variation pattern
description Chagas disease represents a serious problem in public health. This zoonotic pathology is caused by the kinetoplastid Trypanosoma cruzi which displays a high genetic diversity falling into six Discrete Typing Units (TcI–TcVI). In Colombia, the prevalent DTU is TcI with findings of TcII, TcIII and TcIV in low proportions. The aim of this work was to observe the genetic variability within TcI using a multilocus PCR-RFLP strategy. We analyzed 70 single-celled clones from triatomines, reservoirs and humans that were amplified and restricted via ten PCR-RFLPs targets across TcI genome, the restriction fragments were used to construct phylograms according to calculated genetic distances. We obtained five polymorphic targets (1f8, HSP60, HSP70, SAPA and H1) and the consensus tree constructed according to these regions allowed us to observe two well-defined groups with close association to the transmission cycles (domestic/peridomestic and sylvatic) of Chagas disease in Colombia. Our findings allowed us to corroborate the previous reported genotypes based on the intergenic region of mini-exon gene. More studies examining the genetic diversity among T. cruzi I populations must be conducted in order to obtain a better understanding in regions where this DTU is endemic.
format Artículo (Article)
author Ramírez, Juan David
Duque, María Clara
Montilla, Marleny
Cucunubá, Zulma M.
Guhl, Felipe
author_facet Ramírez, Juan David
Duque, María Clara
Montilla, Marleny
Cucunubá, Zulma M.
Guhl, Felipe
author_sort Ramírez, Juan David
title Multilocus PCR-RFLP profiling in Trypanosoma cruzi I highlights an intraspecific genetic variation pattern
title_short Multilocus PCR-RFLP profiling in Trypanosoma cruzi I highlights an intraspecific genetic variation pattern
title_full Multilocus PCR-RFLP profiling in Trypanosoma cruzi I highlights an intraspecific genetic variation pattern
title_fullStr Multilocus PCR-RFLP profiling in Trypanosoma cruzi I highlights an intraspecific genetic variation pattern
title_full_unstemmed Multilocus PCR-RFLP profiling in Trypanosoma cruzi I highlights an intraspecific genetic variation pattern
title_sort multilocus pcr-rflp profiling in trypanosoma cruzi i highlights an intraspecific genetic variation pattern
publisher Elsevier
publishDate 2012
url https://repository.urosario.edu.co/handle/10336/28161
https://doi.org/10.1016/j.meegid.2012.06.018
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