Identifying putative Mycobacterium tuberculosis Rv2004c protein sequences that bind specifically to U937 macrophages and A549 epithelial cells

"Virulence and immunity are still poorly understood in Mycobacterium tuberculo sis.TheH37RvM. tubercu-losis laboratory strain genome has been completely sequenced, and this along with proteomic technologyrepresent powerful tools contributing toward studying the biology of target cell interactio...

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Autores Principales: Forero, Martha, Puentes, Álvaro, Cortés, Jimena, Castillo, Fabio, Vera, Ricardo, Rodríguez, Luis E.
Formato: Artículo (Article)
Lenguaje:Inglés (English)
Publicado: The Protein Society 2005
Materias:
Acceso en línea:https://repository.urosario.edu.co/handle/10336/27843
https://doi.org/10.1110/ps.051592505
id ir-10336-27843
recordtype dspace
spelling ir-10336-278432020-08-19T14:46:42Z Identifying putative Mycobacterium tuberculosis Rv2004c protein sequences that bind specifically to U937 macrophages and A549 epithelial cells Identificación de secuencias de proteínas putativas de Mycobacterium tuberculosis Rv2004c que se unen específicamente a macrófagos U937 y células epiteliales A549 Forero, Martha Puentes, Álvaro Cortés, Jimena Castillo, Fabio Vera, Ricardo Rodríguez, Luis E. Mycobacterium tuberculosis Rv2004c protein High activity binding peptides U937 cells A549 cells "Virulence and immunity are still poorly understood in Mycobacterium tuberculo sis.TheH37RvM. tubercu-losis laboratory strain genome has been completely sequenced, and this along with proteomic technologyrepresent powerful tools contributing toward studying the biology of target cell interaction with a facultativebacillus and designing new strategies for controlling tuberculosis. Rv2004c is a putative M. tuberculosisprotein that could have specific mycobacterial functions. This study has revealed that the encoding gene ispresent in all mycobacterium species belonging to the M. tuberculosis complex. Rv2004c gene transcriptionwas observed in all of this complex’s strains except Mycobacterium bovis and Mycobacterium microti.Rv2004c protein expression was confirmed by using antibodies able to recognize a 54-kDa molecule byimmunoblotting, and its location was detected on the M. tuberculosis surface by transmission electronmicroscopy, suggesting that it is a mycobacterial surface protein. Binding assays led to recognizing highactivity binding peptides (HABP); five HABPs specifically bound to U937 cells, and six specifically boundto A549 cells. HABP circular dichroism suggested that they had an a-helical structure. HABP–target cellinteraction was determined to be specific and saturable ; some of them also displayed greater affinity for A549cells than U937 cells. The critical amino acids directly involved in their interaction with U937 cells were alsodetermined. Two probable receptor molecules were found on U937 cells and five on A549 for the twoHABPs analyzed. These observations have important biological significance for studying bacillus–target cellinteractions and implications for developing strategies for controlling this disease" 2005-11 2020-08-19T14:44:12Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion ISSN: 0961-8368 EISSN: 1469-896X https://repository.urosario.edu.co/handle/10336/27843 https://doi.org/10.1110/ps.051592505 eng info:eu-repo/semantics/openAccess application/pdf The Protein Society John Wiley & Son Protein Science
institution EdocUR - Universidad del Rosario
collection DSpace
language Inglés (English)
topic Mycobacterium tuberculosis
Rv2004c protein
High activity binding peptides
U937 cells
A549 cells
spellingShingle Mycobacterium tuberculosis
Rv2004c protein
High activity binding peptides
U937 cells
A549 cells
Forero, Martha
Puentes, Álvaro
Cortés, Jimena
Castillo, Fabio
Vera, Ricardo
Rodríguez, Luis E.
Identifying putative Mycobacterium tuberculosis Rv2004c protein sequences that bind specifically to U937 macrophages and A549 epithelial cells
description "Virulence and immunity are still poorly understood in Mycobacterium tuberculo sis.TheH37RvM. tubercu-losis laboratory strain genome has been completely sequenced, and this along with proteomic technologyrepresent powerful tools contributing toward studying the biology of target cell interaction with a facultativebacillus and designing new strategies for controlling tuberculosis. Rv2004c is a putative M. tuberculosisprotein that could have specific mycobacterial functions. This study has revealed that the encoding gene ispresent in all mycobacterium species belonging to the M. tuberculosis complex. Rv2004c gene transcriptionwas observed in all of this complex’s strains except Mycobacterium bovis and Mycobacterium microti.Rv2004c protein expression was confirmed by using antibodies able to recognize a 54-kDa molecule byimmunoblotting, and its location was detected on the M. tuberculosis surface by transmission electronmicroscopy, suggesting that it is a mycobacterial surface protein. Binding assays led to recognizing highactivity binding peptides (HABP); five HABPs specifically bound to U937 cells, and six specifically boundto A549 cells. HABP circular dichroism suggested that they had an a-helical structure. HABP–target cellinteraction was determined to be specific and saturable ; some of them also displayed greater affinity for A549cells than U937 cells. The critical amino acids directly involved in their interaction with U937 cells were alsodetermined. Two probable receptor molecules were found on U937 cells and five on A549 for the twoHABPs analyzed. These observations have important biological significance for studying bacillus–target cellinteractions and implications for developing strategies for controlling this disease"
format Artículo (Article)
author Forero, Martha
Puentes, Álvaro
Cortés, Jimena
Castillo, Fabio
Vera, Ricardo
Rodríguez, Luis E.
author_facet Forero, Martha
Puentes, Álvaro
Cortés, Jimena
Castillo, Fabio
Vera, Ricardo
Rodríguez, Luis E.
author_sort Forero, Martha
title Identifying putative Mycobacterium tuberculosis Rv2004c protein sequences that bind specifically to U937 macrophages and A549 epithelial cells
title_short Identifying putative Mycobacterium tuberculosis Rv2004c protein sequences that bind specifically to U937 macrophages and A549 epithelial cells
title_full Identifying putative Mycobacterium tuberculosis Rv2004c protein sequences that bind specifically to U937 macrophages and A549 epithelial cells
title_fullStr Identifying putative Mycobacterium tuberculosis Rv2004c protein sequences that bind specifically to U937 macrophages and A549 epithelial cells
title_full_unstemmed Identifying putative Mycobacterium tuberculosis Rv2004c protein sequences that bind specifically to U937 macrophages and A549 epithelial cells
title_sort identifying putative mycobacterium tuberculosis rv2004c protein sequences that bind specifically to u937 macrophages and a549 epithelial cells
publisher The Protein Society
publishDate 2005
url https://repository.urosario.edu.co/handle/10336/27843
https://doi.org/10.1110/ps.051592505
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