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Distinctive adaptive response to repeated exposure to hydrogen peroxide associated with upregulation of DNA repair genes and cell cycle arrest

Many environmental and physiological stresses are chronic. Thus, cells are constantly exposed to diverse types of genotoxic insults that challenge genome stability, including those that induce oxidative DNA damage. However, most in vitro studies that model cellular response to oxidative stressors em...

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Autores Principales:	Santa-Gonzalez, Gloria A., Gomez-Molina, Andrea, Arcos-Burgos, Mauricio, Meyer, Joel N., Camargo, Mauricio
Formato:	Artículo (Article)
Lenguaje:	Inglés (English)
Publicado:	Elsevier 2016
Materias:	ROS Genotoxicity DNA damage response Up-regulation of DNA repair genes G2/M arrest Adaptation
Acceso en línea:	https://repository.urosario.edu.co/handle/10336/27532 https://doi.org/10.1016/j.redox.2016.07.004

id	ir-10336-27532
recordtype	dspace
spelling	ir-10336-275322021-01-21T08:06:06Z Distinctive adaptive response to repeated exposure to hydrogen peroxide associated with upregulation of DNA repair genes and cell cycle arrest Respuesta adaptativa distintiva a la exposición repetida al peróxido de hidrógeno asociada con la regulación positiva de los genes de reparación del ADN y la detención del ciclo celular Santa-Gonzalez, Gloria A. Gomez-Molina, Andrea Arcos-Burgos, Mauricio Meyer, Joel N. Camargo, Mauricio ROS Genotoxicity DNA damage response Up-regulation of DNA repair genes G2/M arrest Adaptation Many environmental and physiological stresses are chronic. Thus, cells are constantly exposed to diverse types of genotoxic insults that challenge genome stability, including those that induce oxidative DNA damage. However, most in vitro studies that model cellular response to oxidative stressors employ short exposures and/or acute stress models. In this study, we tested the hypothesis that chronic and repeated exposure to a micromolar concentration of hydrogen peroxide (H2O2) could activate DNA damage responses, resulting in cellular adaptations. For this purpose, we developed an in vitro model in which we incubated mouse myoblast cells with a steady concentration of ~50 ?M H2O2 for one hour daily for seven days, followed by a final challenge of a 10 or 20X higher dose of H2O2 (0.5 or 1 mM). We report that intermittent long-term exposure to this oxidative stimulus nearly eliminated cell toxicity and significantly decreased genotoxicity (in particular, a >5-fold decreased in double-strand breaks) resulting from subsequent acute exposure to oxidative stress. This protection was associated with cell cycle arrest in G2/M and induction of expression of nine DNA repair genes. Together, this evidence supports an adaptive response to chronic, low-level oxidative stress that results in genomic protection and up-regulated maintenance of cellular homeostasis. 2016-10 2020-08-19T14:42:36Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion ISSN: 2213-2317 https://repository.urosario.edu.co/handle/10336/27532 https://doi.org/10.1016/j.redox.2016.07.004 eng info:eu-repo/semantics/openAccess application/pdf Elsevier Redox Biology
institution	EdocUR - Universidad del Rosario
collection	DSpace
language	Inglés (English)
topic	ROS Genotoxicity DNA damage response Up-regulation of DNA repair genes G2/M arrest Adaptation
spellingShingle	ROS Genotoxicity DNA damage response Up-regulation of DNA repair genes G2/M arrest Adaptation Santa-Gonzalez, Gloria A. Gomez-Molina, Andrea Arcos-Burgos, Mauricio Meyer, Joel N. Camargo, Mauricio Distinctive adaptive response to repeated exposure to hydrogen peroxide associated with upregulation of DNA repair genes and cell cycle arrest
description	Many environmental and physiological stresses are chronic. Thus, cells are constantly exposed to diverse types of genotoxic insults that challenge genome stability, including those that induce oxidative DNA damage. However, most in vitro studies that model cellular response to oxidative stressors employ short exposures and/or acute stress models. In this study, we tested the hypothesis that chronic and repeated exposure to a micromolar concentration of hydrogen peroxide (H2O2) could activate DNA damage responses, resulting in cellular adaptations. For this purpose, we developed an in vitro model in which we incubated mouse myoblast cells with a steady concentration of ~50 ?M H2O2 for one hour daily for seven days, followed by a final challenge of a 10 or 20X higher dose of H2O2 (0.5 or 1 mM). We report that intermittent long-term exposure to this oxidative stimulus nearly eliminated cell toxicity and significantly decreased genotoxicity (in particular, a >5-fold decreased in double-strand breaks) resulting from subsequent acute exposure to oxidative stress. This protection was associated with cell cycle arrest in G2/M and induction of expression of nine DNA repair genes. Together, this evidence supports an adaptive response to chronic, low-level oxidative stress that results in genomic protection and up-regulated maintenance of cellular homeostasis.
format	Artículo (Article)
author	Santa-Gonzalez, Gloria A. Gomez-Molina, Andrea Arcos-Burgos, Mauricio Meyer, Joel N. Camargo, Mauricio
author_facet	Santa-Gonzalez, Gloria A. Gomez-Molina, Andrea Arcos-Burgos, Mauricio Meyer, Joel N. Camargo, Mauricio
author_sort	Santa-Gonzalez, Gloria A.
title	Distinctive adaptive response to repeated exposure to hydrogen peroxide associated with upregulation of DNA repair genes and cell cycle arrest
title_short	Distinctive adaptive response to repeated exposure to hydrogen peroxide associated with upregulation of DNA repair genes and cell cycle arrest
title_full	Distinctive adaptive response to repeated exposure to hydrogen peroxide associated with upregulation of DNA repair genes and cell cycle arrest
title_fullStr	Distinctive adaptive response to repeated exposure to hydrogen peroxide associated with upregulation of DNA repair genes and cell cycle arrest
title_full_unstemmed	Distinctive adaptive response to repeated exposure to hydrogen peroxide associated with upregulation of DNA repair genes and cell cycle arrest
title_sort	distinctive adaptive response to repeated exposure to hydrogen peroxide associated with upregulation of dna repair genes and cell cycle arrest
publisher	Elsevier
publishDate	2016
url	https://repository.urosario.edu.co/handle/10336/27532 https://doi.org/10.1016/j.redox.2016.07.004
_version_	1689943200912900096
score	12,131701

Distinctive adaptive response to repeated exposure to hydrogen peroxide associated with upregulation of DNA repair genes and cell cycle arrest

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