The identification of two Trypanosoma cruzi I genotypes from domestic and sylvatic transmission cycles in Colombia based on a single polymerase chain reaction amplification of the spliced-leader intergenic region

A single polymerase chain reaction (PCR) reaction targeting the spliced-leader intergenic region of Trypanosoma cruzi I was standardised by amplifying a 231 bp fragment in domestic (TcIDOM) strains or clones and 450 and 550 bp fragments in sylvatic strains or clones. This reaction was validated usin...

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Detalles Bibliográficos
Autores Principales: Villa, Lina Marcela, Guhl, Felipe, Zabala, Daniel, Ramírez, Juan David, Urrea, Daniel Alfonso, Hernández, Diana Carolina, Cucunubá, Zulma, Montilla, Marleny, Carranza, Julio César, Rueda, Karina, Trujillo, Jorge Eduardo, Vallejo, Gustavo Adolfo
Formato: Artículo (Article)
Lenguaje:Inglés (English)
Publicado: Instituto Oswaldo Cruz 2013
Materias:
PCR
Acceso en línea:https://repository.urosario.edu.co/handle/10336/27076
https://doi.org/10.1590/0074-0276130201