Transcriptomic changes across the life cycle of Trypanosoma cruzi II

Trypanosoma cruzi is a flagellated protozoan that causes Chagas disease; it presents a complex life cycle comprising four morphological stages: epimastigote (EP), metacyclic trypomastigote (MT), cell-derived trypomastigote (CDT) and amastigote (AM). Previous transcriptomic studies on three stages (E...

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Autores Principales: Cruz-Saavedra, Lissa, Vallejo, Gustavo A., Guhl, Felipe, David Ramirez, Juan
Formato: Artículo (Article)
Lenguaje:Inglés (English)
Publicado: PeerJ 2020
Materias:
Acceso en línea:https://repository.urosario.edu.co/handle/10336/25053
https://doi.org/10.7717/peerj.8947
id ir-10336-25053
recordtype dspace
spelling ir-10336-250532022-05-02T12:37:21Z Transcriptomic changes across the life cycle of Trypanosoma cruzi II Cruz-Saavedra, Lissa Vallejo, Gustavo A. Guhl, Felipe David Ramirez, Juan Trypanosoma cruzi Tripomastigotes metacíclicos Epimastigotes Tripomastigotes Transcriptómica Caminos Intercambio genético El perfil revela Expresión Mitosis Eupathdb Sombrero de copa Agente Trypanosoma cruzi Metacyclic trypomastigotes Epimastigotes Trypomastigotes Transcriptomic Pathways Genetic exchange Profiling reveals Expression Meiosis Eupathdb Tophat Agent Trypanosoma cruzi is a flagellated protozoan that causes Chagas disease; it presents a complex life cycle comprising four morphological stages: epimastigote (EP), metacyclic trypomastigote (MT), cell-derived trypomastigote (CDT) and amastigote (AM). Previous transcriptomic studies on three stages (EPs, CDTs and AMs) have demonstrated differences in gene expressions among them; however, to the best of our knowledge, no studies have reported on gene expressions in MTs. Therefore, the present study compared differentially expressed genes (DEGs), and signaling pathway reconstruction in EPs, MTs, AMs and CDTs. The results revealed differences in gene expressions in the stages evaluated; these differences were greater between MTs and AMs-PTs. The signaling pathway that presented the highest number of DEGs in all the stages was associated with ribosomes protein profiles, whereas the other related pathways activated were processes related to energy metabolism from glucose, amino acid metabolism, or RNA regulation. However, the role of autophagy in the entire life cycle of T. cruzi and the presence of processes such as meiosis and homologous recombination in MTs (where the expressions of SPO11 and Rad51 plays a role) are crucial. These findings represent an important step towards the full understanding of the molecular basis during the life cycle of T. cruzi. 2020 2020-06-11T13:22:12Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2167-8359 https://repository.urosario.edu.co/handle/10336/25053 https://doi.org/10.7717/peerj.8947 eng info:eu-repo/semantics/openAccess application/pdf PeerJ instname:Universidad del Rosario
institution EdocUR - Universidad del Rosario
collection DSpace
language Inglés (English)
topic Trypanosoma cruzi
Tripomastigotes metacíclicos
Epimastigotes
Tripomastigotes
Transcriptómica
Caminos
Intercambio genético
El perfil revela
Expresión
Mitosis
Eupathdb
Sombrero de copa
Agente
Trypanosoma cruzi
Metacyclic trypomastigotes
Epimastigotes
Trypomastigotes
Transcriptomic
Pathways
Genetic exchange
Profiling reveals
Expression
Meiosis
Eupathdb
Tophat
Agent
spellingShingle Trypanosoma cruzi
Tripomastigotes metacíclicos
Epimastigotes
Tripomastigotes
Transcriptómica
Caminos
Intercambio genético
El perfil revela
Expresión
Mitosis
Eupathdb
Sombrero de copa
Agente
Trypanosoma cruzi
Metacyclic trypomastigotes
Epimastigotes
Trypomastigotes
Transcriptomic
Pathways
Genetic exchange
Profiling reveals
Expression
Meiosis
Eupathdb
Tophat
Agent
Cruz-Saavedra, Lissa
Vallejo, Gustavo A.
Guhl, Felipe
David Ramirez, Juan
Transcriptomic changes across the life cycle of Trypanosoma cruzi II
description Trypanosoma cruzi is a flagellated protozoan that causes Chagas disease; it presents a complex life cycle comprising four morphological stages: epimastigote (EP), metacyclic trypomastigote (MT), cell-derived trypomastigote (CDT) and amastigote (AM). Previous transcriptomic studies on three stages (EPs, CDTs and AMs) have demonstrated differences in gene expressions among them; however, to the best of our knowledge, no studies have reported on gene expressions in MTs. Therefore, the present study compared differentially expressed genes (DEGs), and signaling pathway reconstruction in EPs, MTs, AMs and CDTs. The results revealed differences in gene expressions in the stages evaluated; these differences were greater between MTs and AMs-PTs. The signaling pathway that presented the highest number of DEGs in all the stages was associated with ribosomes protein profiles, whereas the other related pathways activated were processes related to energy metabolism from glucose, amino acid metabolism, or RNA regulation. However, the role of autophagy in the entire life cycle of T. cruzi and the presence of processes such as meiosis and homologous recombination in MTs (where the expressions of SPO11 and Rad51 plays a role) are crucial. These findings represent an important step towards the full understanding of the molecular basis during the life cycle of T. cruzi.
format Artículo (Article)
author Cruz-Saavedra, Lissa
Vallejo, Gustavo A.
Guhl, Felipe
David Ramirez, Juan
author_facet Cruz-Saavedra, Lissa
Vallejo, Gustavo A.
Guhl, Felipe
David Ramirez, Juan
author_sort Cruz-Saavedra, Lissa
title Transcriptomic changes across the life cycle of Trypanosoma cruzi II
title_short Transcriptomic changes across the life cycle of Trypanosoma cruzi II
title_full Transcriptomic changes across the life cycle of Trypanosoma cruzi II
title_fullStr Transcriptomic changes across the life cycle of Trypanosoma cruzi II
title_full_unstemmed Transcriptomic changes across the life cycle of Trypanosoma cruzi II
title_sort transcriptomic changes across the life cycle of trypanosoma cruzi ii
publisher PeerJ
publishDate 2020
url https://repository.urosario.edu.co/handle/10336/25053
https://doi.org/10.7717/peerj.8947
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score 12,131701