Sarconesin: Sarconesiopsis magellanica Blowfly Larval Excretions and Secretions With Antibacterial Properties

"Larval therapy (LT) is an alternative treatment for healing chronic wounds; its action is based on debridement, the removal of bacteria, and stimulating granulation tissue. The most important mechanism when using LT for combating infection depends on larval excretions and secretions (ES). Larv...

Descripción completa

Detalles Bibliográficos
Autores Principales: Díaz-Roa, Andrea, Patarroyo, Manuel A., Bello, Felio J., Da Silva, Pedro I.
Formato: Artículo (Article)
Lenguaje:Inglés (English)
Publicado: Frontiers in Microbiology 2018
Materias:
Acceso en línea:https://repository.urosario.edu.co/handle/10336/25012
https://doi.org/10.3389/fmicb.2018.02249
id ir-10336-25012
recordtype dspace
spelling ir-10336-250122021-01-21T08:13:06Z Sarconesin: Sarconesiopsis magellanica Blowfly Larval Excretions and Secretions With Antibacterial Properties Díaz-Roa, Andrea Patarroyo, Manuel A. Bello, Felio J. Da Silva, Pedro I. antimicrobial peptide Sarconesiopsis magellanica sarconesin larval therapy insect peptide "Larval therapy (LT) is an alternative treatment for healing chronic wounds; its action is based on debridement, the removal of bacteria, and stimulating granulation tissue. The most important mechanism when using LT for combating infection depends on larval excretions and secretions (ES). Larvae are protected against infection by a spectrum of antimicrobial peptides (AMPs); special interest in AMPs has also risen regarding understanding their role in wound healing since they degrade necrotic tissue and kill different bacteria during LT. Sarconesiopsis magellanica (Diptera: Calliphoridae) is a promising medically-important necrophagous fly. This article reports a small AMP being isolated from S. magellanica ES products for the first time; these products were obtained from third-instar larvae taken from a previously-established colony. ES were fractionated by RP-HPLC using C18 columns for the first analysis; the products were then lyophilised and their antimicrobial activity was characterized by incubation with different bacterial strains. These fractions' primary sequences were determined by mass spectrometry and de novo sequencing; five AMPs were obtained, the Sarconesin fraction was characterized and antibacterial activity was tested in different concentrations with minimum inhibitory concentrations starting at 1.2 mu M. Potent inhibitory activity was shown against Gram-negative (Escherichia coli D31, E. coli DH5 alpha, Salmonella enterica ATCC 13314, Pseudomonas aeruginosa 27853) and Gram-positive (Staphylococcus aureus ATCC 29213, S. epidermidis ATCC 12228, Micrococcus luteus A270) bacteria. Sarconesin has a significant similarity with Rho-family GTPases which are important in organelle development, cytoskeletal dynamics, cell movement, and wound repair. The data reported here indicated that Sarconesin could be an alternative candidate for use in therapeutics against Gram-negative and Gram-positive bacterial infections. Our study describes one peptide responsible for antibacterial activity when LT is being used. The results shown here support carrying out further experiments aimed at validating S. magellanica AMPs as novel resources for combating antibacterial resistance." 2018-09-28 2020-06-11T13:22:05Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 1664-302X https://repository.urosario.edu.co/handle/10336/25012 https://doi.org/10.3389/fmicb.2018.02249 eng info:eu-repo/semantics/openAccess application/pdf Frontiers in Microbiology instname:Universidad del Rosario reponame:Repositorio Institucional EdocUR
institution EdocUR - Universidad del Rosario
collection DSpace
language Inglés (English)
topic antimicrobial peptide
Sarconesiopsis magellanica
sarconesin
larval therapy
insect peptide
spellingShingle antimicrobial peptide
Sarconesiopsis magellanica
sarconesin
larval therapy
insect peptide
Díaz-Roa, Andrea
Patarroyo, Manuel A.
Bello, Felio J.
Da Silva, Pedro I.
Sarconesin: Sarconesiopsis magellanica Blowfly Larval Excretions and Secretions With Antibacterial Properties
description "Larval therapy (LT) is an alternative treatment for healing chronic wounds; its action is based on debridement, the removal of bacteria, and stimulating granulation tissue. The most important mechanism when using LT for combating infection depends on larval excretions and secretions (ES). Larvae are protected against infection by a spectrum of antimicrobial peptides (AMPs); special interest in AMPs has also risen regarding understanding their role in wound healing since they degrade necrotic tissue and kill different bacteria during LT. Sarconesiopsis magellanica (Diptera: Calliphoridae) is a promising medically-important necrophagous fly. This article reports a small AMP being isolated from S. magellanica ES products for the first time; these products were obtained from third-instar larvae taken from a previously-established colony. ES were fractionated by RP-HPLC using C18 columns for the first analysis; the products were then lyophilised and their antimicrobial activity was characterized by incubation with different bacterial strains. These fractions' primary sequences were determined by mass spectrometry and de novo sequencing; five AMPs were obtained, the Sarconesin fraction was characterized and antibacterial activity was tested in different concentrations with minimum inhibitory concentrations starting at 1.2 mu M. Potent inhibitory activity was shown against Gram-negative (Escherichia coli D31, E. coli DH5 alpha, Salmonella enterica ATCC 13314, Pseudomonas aeruginosa 27853) and Gram-positive (Staphylococcus aureus ATCC 29213, S. epidermidis ATCC 12228, Micrococcus luteus A270) bacteria. Sarconesin has a significant similarity with Rho-family GTPases which are important in organelle development, cytoskeletal dynamics, cell movement, and wound repair. The data reported here indicated that Sarconesin could be an alternative candidate for use in therapeutics against Gram-negative and Gram-positive bacterial infections. Our study describes one peptide responsible for antibacterial activity when LT is being used. The results shown here support carrying out further experiments aimed at validating S. magellanica AMPs as novel resources for combating antibacterial resistance."
format Artículo (Article)
author Díaz-Roa, Andrea
Patarroyo, Manuel A.
Bello, Felio J.
Da Silva, Pedro I.
author_facet Díaz-Roa, Andrea
Patarroyo, Manuel A.
Bello, Felio J.
Da Silva, Pedro I.
author_sort Díaz-Roa, Andrea
title Sarconesin: Sarconesiopsis magellanica Blowfly Larval Excretions and Secretions With Antibacterial Properties
title_short Sarconesin: Sarconesiopsis magellanica Blowfly Larval Excretions and Secretions With Antibacterial Properties
title_full Sarconesin: Sarconesiopsis magellanica Blowfly Larval Excretions and Secretions With Antibacterial Properties
title_fullStr Sarconesin: Sarconesiopsis magellanica Blowfly Larval Excretions and Secretions With Antibacterial Properties
title_full_unstemmed Sarconesin: Sarconesiopsis magellanica Blowfly Larval Excretions and Secretions With Antibacterial Properties
title_sort sarconesin: sarconesiopsis magellanica blowfly larval excretions and secretions with antibacterial properties
publisher Frontiers in Microbiology
publishDate 2018
url https://repository.urosario.edu.co/handle/10336/25012
https://doi.org/10.3389/fmicb.2018.02249
_version_ 1690577228648153088
score 11,828437