Substance P and Calcitonin gene-related peptide expression in human periodontal ligament after root canal preparation with Reciproc Blue, WaveOne Gold, XP EndoShaper and hand files

Aim: To quantify Substance P (SP) and Calcitonin gene-related peptide (CGRP) expression in healthy human periodontal ligament from premolars after root canal preparation with Reciproc Blue, WaveOne Gold, XP EndoShaper and hand files. Methodology: A total of 50 human periodontal ligament samples were...

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Autores Principales: Caviedes-Bucheli J., Rios-Osorio N., Rey-Rojas M., Laguna-Rivero F., Azuero-Holguin M.M., Diaz L.E., Curtidor H., Castaneda-Ramirez J.J., Munoz H.R.
Formato: Artículo (Article)
Lenguaje:Inglés (English)
Publicado: Blackwell Publishing Ltd 2018
Materias:
Acceso en línea:https://repository.urosario.edu.co/handle/10336/24328
https://doi.org/10.1111/iej.12952
id ir-10336-24328
recordtype dspace
spelling ir-10336-243282022-05-02T12:37:18Z Substance P and Calcitonin gene-related peptide expression in human periodontal ligament after root canal preparation with Reciproc Blue, WaveOne Gold, XP EndoShaper and hand files Caviedes-Bucheli J. Rios-Osorio N. Rey-Rojas M. Laguna-Rivero F. Azuero-Holguin M.M. Diaz L.E. Curtidor H. Castaneda-Ramirez J.J. Munoz H.R. Calcitonin gene related peptide Substance p Adolescent Adult Biosynthesis Colombia Dental device Devices Equipment design Human Mandible Materials testing Metabolism Periodontal ligament Premolar tooth Procedures Root canal preparation Tooth periapical disease Young adult Adolescent Adult Bicuspid Calcitonin gene-related peptide Colombia Dental instruments Equipment design Humans Mandible Materials testing Periapical periodontitis Periodontal ligament Root canal preparation Substance p Young adult Calcitonin gene-related peptide Human periodontal ligament Neurogenic inflammation Neuropeptides Reciproc blue Substance p Symptomatic apical periodontitis Waveone gold Xp-endoshaper Aim: To quantify Substance P (SP) and Calcitonin gene-related peptide (CGRP) expression in healthy human periodontal ligament from premolars after root canal preparation with Reciproc Blue, WaveOne Gold, XP EndoShaper and hand files. Methodology: A total of 50 human periodontal ligament samples were obtained from healthy mandibular premolars where extraction was indicated for orthodontic reasons. Prior to extraction, 40 of these premolars were equally divided into four groups, and root canals were prepared using four different systems: Reciproc Blue, WaveOne Gold, XP EndoShaper and a hand instrumentation technique. The remaining 10 healthy premolars were extracted without treatment and served as a negative control group. All periodontal ligament samples were processed, and SP and CGRP were measured by radioimmunoassay. The Kruskal–Wallis test was used to establish significant differences between groups and LSD post hoc comparisons were also performed. Results: Greater SP and CGRP values were found in the hand instrumentation group, followed by the XP EndoShaper, WaveOne Gold and the Reciproc groups. The lower SP and CGRP values were for the healthy periodontal ligament group. The Kruskal–Wallis test revealed significant differences between groups (P  less than  0.05). Post hoc Least Significant Difference (LSD) tests revealed significant differences (P  less than  0.05) in SP and CGRP expression between all the comparisons except for the Reciproc Blue and WaveOne Gold group (P > 0.05). Conclusion: All the root canal preparation techniques tested increased SP and CGRP expression in human periodontal ligament, with hand files and XP EndoShaper instruments being associated with greater neuropeptide release compared to Reciproc Blue and WaveOne Gold files. © 2018 International Endodontic Journal. Published by John Wiley and Sons Ltd 2018 2020-05-26T00:11:44Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 13652591 01432885 https://repository.urosario.edu.co/handle/10336/24328 https://doi.org/10.1111/iej.12952 eng info:eu-repo/semantics/openAccess application/pdf Blackwell Publishing Ltd instname:Universidad del Rosario
institution EdocUR - Universidad del Rosario
collection DSpace
language Inglés (English)
topic Calcitonin gene related peptide
Substance p
Adolescent
Adult
Biosynthesis
Colombia
Dental device
Devices
Equipment design
Human
Mandible
Materials testing
Metabolism
Periodontal ligament
Premolar tooth
Procedures
Root canal preparation
Tooth periapical disease
Young adult
Adolescent
Adult
Bicuspid
Calcitonin gene-related peptide
Colombia
Dental instruments
Equipment design
Humans
Mandible
Materials testing
Periapical periodontitis
Periodontal ligament
Root canal preparation
Substance p
Young adult
Calcitonin gene-related peptide
Human periodontal ligament
Neurogenic inflammation
Neuropeptides
Reciproc blue
Substance p
Symptomatic apical periodontitis
Waveone gold
Xp-endoshaper
spellingShingle Calcitonin gene related peptide
Substance p
Adolescent
Adult
Biosynthesis
Colombia
Dental device
Devices
Equipment design
Human
Mandible
Materials testing
Metabolism
Periodontal ligament
Premolar tooth
Procedures
Root canal preparation
Tooth periapical disease
Young adult
Adolescent
Adult
Bicuspid
Calcitonin gene-related peptide
Colombia
Dental instruments
Equipment design
Humans
Mandible
Materials testing
Periapical periodontitis
Periodontal ligament
Root canal preparation
Substance p
Young adult
Calcitonin gene-related peptide
Human periodontal ligament
Neurogenic inflammation
Neuropeptides
Reciproc blue
Substance p
Symptomatic apical periodontitis
Waveone gold
Xp-endoshaper
Caviedes-Bucheli J.
Rios-Osorio N.
Rey-Rojas M.
Laguna-Rivero F.
Azuero-Holguin M.M.
Diaz L.E.
Curtidor H.
Castaneda-Ramirez J.J.
Munoz H.R.
Substance P and Calcitonin gene-related peptide expression in human periodontal ligament after root canal preparation with Reciproc Blue, WaveOne Gold, XP EndoShaper and hand files
description Aim: To quantify Substance P (SP) and Calcitonin gene-related peptide (CGRP) expression in healthy human periodontal ligament from premolars after root canal preparation with Reciproc Blue, WaveOne Gold, XP EndoShaper and hand files. Methodology: A total of 50 human periodontal ligament samples were obtained from healthy mandibular premolars where extraction was indicated for orthodontic reasons. Prior to extraction, 40 of these premolars were equally divided into four groups, and root canals were prepared using four different systems: Reciproc Blue, WaveOne Gold, XP EndoShaper and a hand instrumentation technique. The remaining 10 healthy premolars were extracted without treatment and served as a negative control group. All periodontal ligament samples were processed, and SP and CGRP were measured by radioimmunoassay. The Kruskal–Wallis test was used to establish significant differences between groups and LSD post hoc comparisons were also performed. Results: Greater SP and CGRP values were found in the hand instrumentation group, followed by the XP EndoShaper, WaveOne Gold and the Reciproc groups. The lower SP and CGRP values were for the healthy periodontal ligament group. The Kruskal–Wallis test revealed significant differences between groups (P  less than  0.05). Post hoc Least Significant Difference (LSD) tests revealed significant differences (P  less than  0.05) in SP and CGRP expression between all the comparisons except for the Reciproc Blue and WaveOne Gold group (P > 0.05). Conclusion: All the root canal preparation techniques tested increased SP and CGRP expression in human periodontal ligament, with hand files and XP EndoShaper instruments being associated with greater neuropeptide release compared to Reciproc Blue and WaveOne Gold files. © 2018 International Endodontic Journal. Published by John Wiley and Sons Ltd
format Artículo (Article)
author Caviedes-Bucheli J.
Rios-Osorio N.
Rey-Rojas M.
Laguna-Rivero F.
Azuero-Holguin M.M.
Diaz L.E.
Curtidor H.
Castaneda-Ramirez J.J.
Munoz H.R.
author_facet Caviedes-Bucheli J.
Rios-Osorio N.
Rey-Rojas M.
Laguna-Rivero F.
Azuero-Holguin M.M.
Diaz L.E.
Curtidor H.
Castaneda-Ramirez J.J.
Munoz H.R.
author_sort Caviedes-Bucheli J.
title Substance P and Calcitonin gene-related peptide expression in human periodontal ligament after root canal preparation with Reciproc Blue, WaveOne Gold, XP EndoShaper and hand files
title_short Substance P and Calcitonin gene-related peptide expression in human periodontal ligament after root canal preparation with Reciproc Blue, WaveOne Gold, XP EndoShaper and hand files
title_full Substance P and Calcitonin gene-related peptide expression in human periodontal ligament after root canal preparation with Reciproc Blue, WaveOne Gold, XP EndoShaper and hand files
title_fullStr Substance P and Calcitonin gene-related peptide expression in human periodontal ligament after root canal preparation with Reciproc Blue, WaveOne Gold, XP EndoShaper and hand files
title_full_unstemmed Substance P and Calcitonin gene-related peptide expression in human periodontal ligament after root canal preparation with Reciproc Blue, WaveOne Gold, XP EndoShaper and hand files
title_sort substance p and calcitonin gene-related peptide expression in human periodontal ligament after root canal preparation with reciproc blue, waveone gold, xp endoshaper and hand files
publisher Blackwell Publishing Ltd
publishDate 2018
url https://repository.urosario.edu.co/handle/10336/24328
https://doi.org/10.1111/iej.12952
_version_ 1740173048657477632
score 12,131701