Vista Equipo: Peptides derived from Mycobacterium tuberculosis Rv2301 protein are involved in invasion to human epithelial cells and macrophages

Peptides derived from Mycobacterium tuberculosis Rv2301 protein are involved in invasion to human epithelial cells and macrophages

The specific function of putative cut2 protein (or CFP25), encoded by the Rv2301 gene from Mycobacterium tuberculosis H37Rv, has not been identified yet. The aim of this study was to assess some of CFP25 characteristics and its possible biological role in Mycobacterium tuberculosis H37Rv invasion pr...

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Autores Principales:	Ocampo, M., Rodríguez, D. M., Curtidor, H., Vanegas, M., Patarroyo, M. A., Patarroyo, M. E.
Formato:	Artículo (Article)
Lenguaje:	Inglés (English)
Publicado:	2012
Materias:	Bacterial protein Culture filtrate protein 25 Cytochalasin d Rv2301 protein Unclassified drug Amino acid sequence Article Bacterial strain Binding affinity Cell invasion Cell surface Controlled study Dissociation constant Human Human cell Immunoelectron microscopy Internalization Lung alveolus epithelium Macrophage Mycobacterium tuberculosis Nonhuman Nucleotide sequence Priority journal Protein binding Western blotting Bacterial proteins Binding sites Cytochalasins Epithelial cells Humans Kinetics Macrophages Molecular sequence data Organ specificity Peptides Bacilli (class) Capra hircus Cutinase High activity binding peptides Rv2301 western tumor pulmonary bacterial immunoelectron Antigens Blotting Cell line Microscopy Tuberculosis
Acceso en línea:	https://repository.urosario.edu.co/handle/10336/24240 https://doi.org/10.1007/s00726-011-0938-7

id	ir-10336-24240
recordtype	dspace
spelling	ir-10336-242402022-05-02T12:37:14Z Peptides derived from Mycobacterium tuberculosis Rv2301 protein are involved in invasion to human epithelial cells and macrophages Ocampo, M. Rodríguez, D. M. Curtidor, H. Vanegas, M. Patarroyo, M. A. Patarroyo, M. E. Bacterial protein Culture filtrate protein 25 Cytochalasin d Rv2301 protein Unclassified drug Amino acid sequence Article Bacterial strain Binding affinity Cell invasion Cell surface Controlled study Dissociation constant Human Human cell Immunoelectron microscopy Internalization Lung alveolus epithelium Macrophage Mycobacterium tuberculosis Nonhuman Nucleotide sequence Priority journal Protein binding Western blotting Amino acid sequence Bacterial proteins Binding sites Cytochalasins Epithelial cells Humans Kinetics Macrophages Molecular sequence data Mycobacterium tuberculosis Organ specificity Peptides Protein binding Bacilli (class) Capra hircus Mycobacterium tuberculosis Cutinase High activity binding peptides Mycobacterium tuberculosis Rv2301 western tumor pulmonary bacterial immunoelectron Antigens Blotting Cell line Microscopy Tuberculosis The specific function of putative cut2 protein (or CFP25), encoded by the Rv2301 gene from Mycobacterium tuberculosis H37Rv, has not been identified yet. The aim of this study was to assess some of CFP25 characteristics and its possible biological role in Mycobacterium tuberculosis H37Rv invasion process to target cells. Molecular assays indicated that the gene encoding Rv2301 is present and transcribed in M. tuberculosis complex strains. The presence of Rv2301 protein over the bacilli surface was confirmed by Western blot and immunoelectron microscopy analyses, using goats sera inoculated with synthetic peptides derived from Rv2301 protein. Receptor-ligand binding assays with carcinomic human alveolar basal epithelial cells (A549) and macrophages derived from human histolytic lymphoma monocytes (U937) allowed us to identify five high activity binding peptides (HABPs) in both cell lines, and two additional HABPs only in A549 cells. U937 HABPs binding interactions were characterized by saturation assays, finding dissociation constants (K d) within the nanomolar range and positive cooperativity (n H and gt; 1). Inhibition assays were performed to assess the possible biological role of Rv2301 identified HABPs, finding that some of them were able to inhibit invasion at a 5 ?M concentration, compared with the cytochalasin control. On the other hand, HABPs, and especially HABP 36507 located at the N-terminus of the protein, facilitated the internalization of fluorescent latex beads into A549 cells. These findings are of vital importance for the rational selection of Rv2301 HABPs, to be included as components of an antituberculosis vaccine. © 2011 Springer-Verlag. 2012 2020-05-26T00:10:36Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 09394451 14382199 https://repository.urosario.edu.co/handle/10336/24240 https://doi.org/10.1007/s00726-011-0938-7 eng info:eu-repo/semantics/openAccess application/pdf instname:Universidad del Rosario
institution	EdocUR - Universidad del Rosario
collection	DSpace
language	Inglés (English)
topic	Bacterial protein Culture filtrate protein 25 Cytochalasin d Rv2301 protein Unclassified drug Amino acid sequence Article Bacterial strain Binding affinity Cell invasion Cell surface Controlled study Dissociation constant Human Human cell Immunoelectron microscopy Internalization Lung alveolus epithelium Macrophage Mycobacterium tuberculosis Nonhuman Nucleotide sequence Priority journal Protein binding Western blotting Amino acid sequence Bacterial proteins Binding sites Cytochalasins Epithelial cells Humans Kinetics Macrophages Molecular sequence data Mycobacterium tuberculosis Organ specificity Peptides Protein binding Bacilli (class) Capra hircus Mycobacterium tuberculosis Cutinase High activity binding peptides Mycobacterium tuberculosis Rv2301 western tumor pulmonary bacterial immunoelectron Antigens Blotting Cell line Microscopy Tuberculosis
spellingShingle	Bacterial protein Culture filtrate protein 25 Cytochalasin d Rv2301 protein Unclassified drug Amino acid sequence Article Bacterial strain Binding affinity Cell invasion Cell surface Controlled study Dissociation constant Human Human cell Immunoelectron microscopy Internalization Lung alveolus epithelium Macrophage Mycobacterium tuberculosis Nonhuman Nucleotide sequence Priority journal Protein binding Western blotting Amino acid sequence Bacterial proteins Binding sites Cytochalasins Epithelial cells Humans Kinetics Macrophages Molecular sequence data Mycobacterium tuberculosis Organ specificity Peptides Protein binding Bacilli (class) Capra hircus Mycobacterium tuberculosis Cutinase High activity binding peptides Mycobacterium tuberculosis Rv2301 western tumor pulmonary bacterial immunoelectron Antigens Blotting Cell line Microscopy Tuberculosis Ocampo, M. Rodríguez, D. M. Curtidor, H. Vanegas, M. Patarroyo, M. A. Patarroyo, M. E. Peptides derived from Mycobacterium tuberculosis Rv2301 protein are involved in invasion to human epithelial cells and macrophages
description	The specific function of putative cut2 protein (or CFP25), encoded by the Rv2301 gene from Mycobacterium tuberculosis H37Rv, has not been identified yet. The aim of this study was to assess some of CFP25 characteristics and its possible biological role in Mycobacterium tuberculosis H37Rv invasion process to target cells. Molecular assays indicated that the gene encoding Rv2301 is present and transcribed in M. tuberculosis complex strains. The presence of Rv2301 protein over the bacilli surface was confirmed by Western blot and immunoelectron microscopy analyses, using goats sera inoculated with synthetic peptides derived from Rv2301 protein. Receptor-ligand binding assays with carcinomic human alveolar basal epithelial cells (A549) and macrophages derived from human histolytic lymphoma monocytes (U937) allowed us to identify five high activity binding peptides (HABPs) in both cell lines, and two additional HABPs only in A549 cells. U937 HABPs binding interactions were characterized by saturation assays, finding dissociation constants (K d) within the nanomolar range and positive cooperativity (n H and gt; 1). Inhibition assays were performed to assess the possible biological role of Rv2301 identified HABPs, finding that some of them were able to inhibit invasion at a 5 ?M concentration, compared with the cytochalasin control. On the other hand, HABPs, and especially HABP 36507 located at the N-terminus of the protein, facilitated the internalization of fluorescent latex beads into A549 cells. These findings are of vital importance for the rational selection of Rv2301 HABPs, to be included as components of an antituberculosis vaccine. © 2011 Springer-Verlag.
format	Artículo (Article)
author	Ocampo, M. Rodríguez, D. M. Curtidor, H. Vanegas, M. Patarroyo, M. A. Patarroyo, M. E.
author_facet	Ocampo, M. Rodríguez, D. M. Curtidor, H. Vanegas, M. Patarroyo, M. A. Patarroyo, M. E.
author_sort	Ocampo, M.
title	Peptides derived from Mycobacterium tuberculosis Rv2301 protein are involved in invasion to human epithelial cells and macrophages
title_short	Peptides derived from Mycobacterium tuberculosis Rv2301 protein are involved in invasion to human epithelial cells and macrophages
title_full	Peptides derived from Mycobacterium tuberculosis Rv2301 protein are involved in invasion to human epithelial cells and macrophages
title_fullStr	Peptides derived from Mycobacterium tuberculosis Rv2301 protein are involved in invasion to human epithelial cells and macrophages
title_full_unstemmed	Peptides derived from Mycobacterium tuberculosis Rv2301 protein are involved in invasion to human epithelial cells and macrophages
title_sort	peptides derived from mycobacterium tuberculosis rv2301 protein are involved in invasion to human epithelial cells and macrophages
publishDate	2012
url	https://repository.urosario.edu.co/handle/10336/24240 https://doi.org/10.1007/s00726-011-0938-7
_version_	1740172406294577152
score	12,131701

Peptides derived from Mycobacterium tuberculosis Rv2301 protein are involved in invasion to human epithelial cells and macrophages

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