Imagen de Portada

Anaerobic sulfatase maturase AslB from Escherichia coli activates human recombinant iduronate-2-sulfate sulfatase (IDS) and N-acetylgalactosamine-6-sulfate sulfatase (GALNS)

Maturation of type I sulfatases requires the conversion of the cysteine (Cys) or serine (Ser) present in the active site to formylglycine (FGly). This activation represents a limiting step during the production of recombinant sulfatases in bacteria and eukaryotic hosts. AslB, YdeM and YidF have been...

Descripción completa

Detalles Bibliográficos
Autores Principales: Alméciga-Díaz C.J., Tolosa-Díaz A.D., Pimentel L.N., Bonilla Y.A., Rodríguez-López A., Espejo-Mojica A.J., Patiño J.D., Sánchez O.F., Gonzalez-Santos J.
Formato: Artículo (Article)
Lenguaje:Inglés (English)
Publicado: Elsevier B.V. 2017
Materias:
Anaerobic sulfatase maturase aslb
Cysteine
Iduronate 2 sulfatase
N acetylgalactosamine 6 sulfatase
Recombinant enzyme
Serine
Sulfatase
Unclassified drug
Escherichia coli protein
Glycoprotein
N acetylgalactosamine 4 sulfatase
Protein binding
Recombinant protein
Amino acid sequence
Article
Binding affinity
Controlled study
Enzyme activation
Enzyme active site
Enzyme activity
Escherichia coli
Molecular docking
Molecular dynamics
Molecular model
Nonhuman
Priority journal
Protein expression
Protein motif
Protein protein interaction
Chemistry
Enzymology
Human
Metabolism
Catalytic Domain
Chondroitinsulfatases
Enzyme Activation
Escherichia coli Proteins
Glycoproteins
Humans
Molecular Docking Simulation
Molecular Dynamics Simulation
Protein Binding
Recombinant Proteins
Sulfatases
Aslb
Formylglycine-generating enzymes
Iduronate-2-sulfate sulfatase
N-acetylgalactosamine-6-sulfate sulfatase
Molecular
human
GALNS protein
IDS protein
Models
Acceso en línea:https://repository.urosario.edu.co/handle/10336/24133
https://doi.org/10.1016/j.gene.2017.08.043

Internet

https://repository.urosario.edu.co/handle/10336/24133
https://doi.org/10.1016/j.gene.2017.08.043

Ejemplares similares