Plasmodium vivax Cell Traversal Protein for Ookinetes and Sporozoites (CelTOS) Functionally Restricted Regions Are Involved in Specific Host-Pathogen Interactions

Following the injection of Plasmodium sporozoites by a female Anopheles mosquito into the dermis, they become engaged on a long journey to hepatic tissue where they must migrate through different types of cell to become established in parasitophorous vacuoles in hepatocytes. Studies have shown that...

Descripción completa

Detalles Bibliográficos
Autores Principales: Arévalo-Pinzón, Gabriela, Garzón-Ospina, Diego, Pulido, Fredy A., Bermúdez, Maritza, Forero-Rodríguez, Johanna, Rodríguez-Mesa, Xandy M., Reyes-Guarín, Leidy P., Suárez, Carlos F., Patarroyo, Manuel A.
Formato: Artículo (Article)
Lenguaje:Inglés (English)
Publicado: Frontiers Media S.A. 2020
Materias:
P
Acceso en línea:https://repository.urosario.edu.co/handle/10336/24078
https://doi.org/10.3389/fcimb.2020.00119
id ir-10336-24078
recordtype dspace
spelling ir-10336-240782022-05-02T12:37:14Z Plasmodium vivax Cell Traversal Protein for Ookinetes and Sporozoites (CelTOS) Functionally Restricted Regions Are Involved in Specific Host-Pathogen Interactions Arévalo-Pinzón, Gabriela Garzón-Ospina, Diego Pulido, Fredy A. Bermúdez, Maritza Forero-Rodríguez, Johanna Rodríguez-Mesa, Xandy M. Reyes-Guarín, Leidy P. Suárez, Carlos F. Patarroyo, Manuel A. Amino acid sequence Antigenicity Article Bioinformatics Blood sampling Cell selection Cell surface Clinical article Controlled study Crystal structure Dna extraction Dna purification Enzyme linked immunosorbent assay Female Flow cytometry Gene amplification Gene frequency Gene sequence Genetic polymorphism Genetic selection Genetic variability Host pathogen interaction Human Human cell Immunofluorescence test Isotope labeling Nonhuman Plasmid Plasmodium Plasmodium vivax Plasmodium vivax malaria Protein binding Protein expression Protein structure Sequence analysis Single nucleotide polymorphism Sporozoite Western blotting Celtos Malaria Multi-epitope multi-stage vaccine P Vivax Sporozoite Following the injection of Plasmodium sporozoites by a female Anopheles mosquito into the dermis, they become engaged on a long journey to hepatic tissue where they must migrate through different types of cell to become established in parasitophorous vacuoles in hepatocytes. Studies have shown that proteins such as cell traversal protein for Plasmodium ookinetes and sporozoites (CelTOS) play a crucial role in cell-traversal ability. Although CelTOS has been extensively studied in various species and included in pre-clinical assays it remains unknown which P. vivax CelTOS (PvCelTOS) regions are key in its interaction with traversed or target cells (Kupffer or hepatocytes) and what type of pressure, association and polymorphism these important regions could have to improve their candidacy as important vaccine antigens. This work has described producing a recombinant PvCelTOS which was recognized by ~30% P. vivax-infected individuals, thereby confirming its ability for inducing a natural immune response. PvCelTOS' genetic diversity in Colombia and its ability to interact with HeLa (traversal cell) and/or HepG2 cell (target cell) external membrane have been assessed. One region in the PvCelTOS amino-terminal region and another in its C-terminus were seen to be participating in host-pathogen interactions. These regions had important functional constraint signals (? less than 0.3 and several sites under negative selection) and were able to inhibit specific rPvCelTOS binding to HeLa cells. This led to suggesting that sequences between aa 41–60 (40833) and 141–160 (40838) represent promising candidates for an anti-P. vivax subunit-based vaccine. © Copyright © 2020 Arévalo-Pinzón, Garzón-Ospina, Pulido, Bermúdez, Forero-Rodríguez, Rodríguez-Mesa, Reyes-Guarín, Suárez and Patarroyo. 2020 2020-05-26T00:08:20Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 22352988 https://repository.urosario.edu.co/handle/10336/24078 https://doi.org/10.3389/fcimb.2020.00119 eng info:eu-repo/semantics/openAccess application/pdf Frontiers Media S.A. instname:Universidad del Rosario
institution EdocUR - Universidad del Rosario
collection DSpace
language Inglés (English)
topic Amino acid sequence
Antigenicity
Article
Bioinformatics
Blood sampling
Cell selection
Cell surface
Clinical article
Controlled study
Crystal structure
Dna extraction
Dna purification
Enzyme linked immunosorbent assay
Female
Flow cytometry
Gene amplification
Gene frequency
Gene sequence
Genetic polymorphism
Genetic selection
Genetic variability
Host pathogen interaction
Human
Human cell
Immunofluorescence test
Isotope labeling
Nonhuman
Plasmid
Plasmodium
Plasmodium vivax
Plasmodium vivax malaria
Protein binding
Protein expression
Protein structure
Sequence analysis
Single nucleotide polymorphism
Sporozoite
Western blotting
Celtos
Malaria
Multi-epitope multi-stage vaccine
P
Vivax
Sporozoite
spellingShingle Amino acid sequence
Antigenicity
Article
Bioinformatics
Blood sampling
Cell selection
Cell surface
Clinical article
Controlled study
Crystal structure
Dna extraction
Dna purification
Enzyme linked immunosorbent assay
Female
Flow cytometry
Gene amplification
Gene frequency
Gene sequence
Genetic polymorphism
Genetic selection
Genetic variability
Host pathogen interaction
Human
Human cell
Immunofluorescence test
Isotope labeling
Nonhuman
Plasmid
Plasmodium
Plasmodium vivax
Plasmodium vivax malaria
Protein binding
Protein expression
Protein structure
Sequence analysis
Single nucleotide polymorphism
Sporozoite
Western blotting
Celtos
Malaria
Multi-epitope multi-stage vaccine
P
Vivax
Sporozoite
Arévalo-Pinzón, Gabriela
Garzón-Ospina, Diego
Pulido, Fredy A.
Bermúdez, Maritza
Forero-Rodríguez, Johanna
Rodríguez-Mesa, Xandy M.
Reyes-Guarín, Leidy P.
Suárez, Carlos F.
Patarroyo, Manuel A.
Plasmodium vivax Cell Traversal Protein for Ookinetes and Sporozoites (CelTOS) Functionally Restricted Regions Are Involved in Specific Host-Pathogen Interactions
description Following the injection of Plasmodium sporozoites by a female Anopheles mosquito into the dermis, they become engaged on a long journey to hepatic tissue where they must migrate through different types of cell to become established in parasitophorous vacuoles in hepatocytes. Studies have shown that proteins such as cell traversal protein for Plasmodium ookinetes and sporozoites (CelTOS) play a crucial role in cell-traversal ability. Although CelTOS has been extensively studied in various species and included in pre-clinical assays it remains unknown which P. vivax CelTOS (PvCelTOS) regions are key in its interaction with traversed or target cells (Kupffer or hepatocytes) and what type of pressure, association and polymorphism these important regions could have to improve their candidacy as important vaccine antigens. This work has described producing a recombinant PvCelTOS which was recognized by ~30% P. vivax-infected individuals, thereby confirming its ability for inducing a natural immune response. PvCelTOS' genetic diversity in Colombia and its ability to interact with HeLa (traversal cell) and/or HepG2 cell (target cell) external membrane have been assessed. One region in the PvCelTOS amino-terminal region and another in its C-terminus were seen to be participating in host-pathogen interactions. These regions had important functional constraint signals (? less than 0.3 and several sites under negative selection) and were able to inhibit specific rPvCelTOS binding to HeLa cells. This led to suggesting that sequences between aa 41–60 (40833) and 141–160 (40838) represent promising candidates for an anti-P. vivax subunit-based vaccine. © Copyright © 2020 Arévalo-Pinzón, Garzón-Ospina, Pulido, Bermúdez, Forero-Rodríguez, Rodríguez-Mesa, Reyes-Guarín, Suárez and Patarroyo.
format Artículo (Article)
author Arévalo-Pinzón, Gabriela
Garzón-Ospina, Diego
Pulido, Fredy A.
Bermúdez, Maritza
Forero-Rodríguez, Johanna
Rodríguez-Mesa, Xandy M.
Reyes-Guarín, Leidy P.
Suárez, Carlos F.
Patarroyo, Manuel A.
author_facet Arévalo-Pinzón, Gabriela
Garzón-Ospina, Diego
Pulido, Fredy A.
Bermúdez, Maritza
Forero-Rodríguez, Johanna
Rodríguez-Mesa, Xandy M.
Reyes-Guarín, Leidy P.
Suárez, Carlos F.
Patarroyo, Manuel A.
author_sort Arévalo-Pinzón, Gabriela
title Plasmodium vivax Cell Traversal Protein for Ookinetes and Sporozoites (CelTOS) Functionally Restricted Regions Are Involved in Specific Host-Pathogen Interactions
title_short Plasmodium vivax Cell Traversal Protein for Ookinetes and Sporozoites (CelTOS) Functionally Restricted Regions Are Involved in Specific Host-Pathogen Interactions
title_full Plasmodium vivax Cell Traversal Protein for Ookinetes and Sporozoites (CelTOS) Functionally Restricted Regions Are Involved in Specific Host-Pathogen Interactions
title_fullStr Plasmodium vivax Cell Traversal Protein for Ookinetes and Sporozoites (CelTOS) Functionally Restricted Regions Are Involved in Specific Host-Pathogen Interactions
title_full_unstemmed Plasmodium vivax Cell Traversal Protein for Ookinetes and Sporozoites (CelTOS) Functionally Restricted Regions Are Involved in Specific Host-Pathogen Interactions
title_sort plasmodium vivax cell traversal protein for ookinetes and sporozoites (celtos) functionally restricted regions are involved in specific host-pathogen interactions
publisher Frontiers Media S.A.
publishDate 2020
url https://repository.urosario.edu.co/handle/10336/24078
https://doi.org/10.3389/fcimb.2020.00119
_version_ 1740172319724142592
score 12,131701