Functional, biochemical and 3D studies of Mycobacterium tuberculosis protein peptides for an effective anti-tuberculosis vaccine
Tuberculosis (TB) is an air-born, transmissible disease, having an estimated 9.4 million new TB cases worldwide in 2009. Eventual control of this disease by developing a safe and efficient new vaccine able to detain its spread will have an enormous impact on public health policy. Selecting potential...
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ir-10336-237822022-05-02T12:37:19Z Functional, biochemical and 3D studies of Mycobacterium tuberculosis protein peptides for an effective anti-tuberculosis vaccine Ocampo, Marisol Patarroyo, Manuel A. Vanegas, Magnolia Alba, Martha P. Patarroyo, Manuel E. Bacterial antigen Bacterial protein Bcg vaccine Rv 0180 c protein Rv 0227 c protein Rv 0679 c protein Rv 1490 protein Rv 1510 c protein Rv 1980 c protein Rv 2004 c protein Rv 2301 protein Rv 2536 protein Rv 2560 protein Rv 2707 protein Rv 2969c protein Rv 3166 c protein Rv 3481 c protein Rv 3629 c protein Rv 3630 protein Rv 3804 c protein Rv 3910 protein Synthetic peptide Unclassified drug Bioinformatics Immune response Molecular biology Mycobacterium tuberculosis Nonhuman Priority journal Protein analysis Protein binding Protein function Proteomics Review Three dimensional imaging Bacterial adhesion Bacterial proteins Cell line Epithelial cells Humans Macrophages Mycobacterium tuberculosis Tuberculosis vaccines Mycobacterium tuberculosis surface protein Receptor-ligand interaction Subunit-vaccine Synthetic-peptides bacterial Antigens Tuberculosis (TB) is an air-born, transmissible disease, having an estimated 9.4 million new TB cases worldwide in 2009. Eventual control of this disease by developing a safe and efficient new vaccine able to detain its spread will have an enormous impact on public health policy. Selecting potential antigens to be included in a multi-epitope, minimal subunit-based, chemically-synthesized vaccine containing the minimum sequences needed for blocking mycobacterial interaction with host cells is a complex task due to the multiple mechanisms involved in M. tuberculosis infection and the mycobacterium's immune evasion mechanisms. Our methodology, described here takes into account a highly robust, specific, sensitive and functional approach to the search for potential epitopes to be included in an anti-TB vaccine; it has been based on identifying short mycobacterial protein fragments using synthetic peptides having high affinity interaction with alveolar epithelial cells (A549) and monocyte-derived macrophages (U937) which are able to block the microorganism's entry to target cells in in vitro assays. This manuscript presents a review of the results obtained with some of the MTB H37Rv proteins studied to date, aimed at using these high activity binding peptides (HABPs) as platforms to be included in a minimal subunit-based, multiepitope, chemically-synthesized, antituberculosis vaccine. © 2014 Informa Healthcare USA, Inc. All rights reserved: reproduction in whole or part not permitted. 2014 2020-05-26T00:05:21Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 15497828 1040841X https://repository.urosario.edu.co/handle/10336/23782 https://doi.org/10.3109/1040841X.2013.763221 eng info:eu-repo/semantics/openAccess application/pdf instname:Universidad del Rosario |
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EdocUR - Universidad del Rosario |
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DSpace |
language |
Inglés (English) |
topic |
Bacterial antigen Bacterial protein Bcg vaccine Rv 0180 c protein Rv 0227 c protein Rv 0679 c protein Rv 1490 protein Rv 1510 c protein Rv 1980 c protein Rv 2004 c protein Rv 2301 protein Rv 2536 protein Rv 2560 protein Rv 2707 protein Rv 2969c protein Rv 3166 c protein Rv 3481 c protein Rv 3629 c protein Rv 3630 protein Rv 3804 c protein Rv 3910 protein Synthetic peptide Unclassified drug Bioinformatics Immune response Molecular biology Mycobacterium tuberculosis Nonhuman Priority journal Protein analysis Protein binding Protein function Proteomics Review Three dimensional imaging Bacterial adhesion Bacterial proteins Cell line Epithelial cells Humans Macrophages Mycobacterium tuberculosis Tuberculosis vaccines Mycobacterium tuberculosis surface protein Receptor-ligand interaction Subunit-vaccine Synthetic-peptides bacterial Antigens |
spellingShingle |
Bacterial antigen Bacterial protein Bcg vaccine Rv 0180 c protein Rv 0227 c protein Rv 0679 c protein Rv 1490 protein Rv 1510 c protein Rv 1980 c protein Rv 2004 c protein Rv 2301 protein Rv 2536 protein Rv 2560 protein Rv 2707 protein Rv 2969c protein Rv 3166 c protein Rv 3481 c protein Rv 3629 c protein Rv 3630 protein Rv 3804 c protein Rv 3910 protein Synthetic peptide Unclassified drug Bioinformatics Immune response Molecular biology Mycobacterium tuberculosis Nonhuman Priority journal Protein analysis Protein binding Protein function Proteomics Review Three dimensional imaging Bacterial adhesion Bacterial proteins Cell line Epithelial cells Humans Macrophages Mycobacterium tuberculosis Tuberculosis vaccines Mycobacterium tuberculosis surface protein Receptor-ligand interaction Subunit-vaccine Synthetic-peptides bacterial Antigens Ocampo, Marisol Patarroyo, Manuel A. Vanegas, Magnolia Alba, Martha P. Patarroyo, Manuel E. Functional, biochemical and 3D studies of Mycobacterium tuberculosis protein peptides for an effective anti-tuberculosis vaccine |
description |
Tuberculosis (TB) is an air-born, transmissible disease, having an estimated 9.4 million new TB cases worldwide in 2009. Eventual control of this disease by developing a safe and efficient new vaccine able to detain its spread will have an enormous impact on public health policy. Selecting potential antigens to be included in a multi-epitope, minimal subunit-based, chemically-synthesized vaccine containing the minimum sequences needed for blocking mycobacterial interaction with host cells is a complex task due to the multiple mechanisms involved in M. tuberculosis infection and the mycobacterium's immune evasion mechanisms. Our methodology, described here takes into account a highly robust, specific, sensitive and functional approach to the search for potential epitopes to be included in an anti-TB vaccine; it has been based on identifying short mycobacterial protein fragments using synthetic peptides having high affinity interaction with alveolar epithelial cells (A549) and monocyte-derived macrophages (U937) which are able to block the microorganism's entry to target cells in in vitro assays. This manuscript presents a review of the results obtained with some of the MTB H37Rv proteins studied to date, aimed at using these high activity binding peptides (HABPs) as platforms to be included in a minimal subunit-based, multiepitope, chemically-synthesized, antituberculosis vaccine. © 2014 Informa Healthcare USA, Inc. All rights reserved: reproduction in whole or part not permitted. |
format |
Artículo (Article) |
author |
Ocampo, Marisol Patarroyo, Manuel A. Vanegas, Magnolia Alba, Martha P. Patarroyo, Manuel E. |
author_facet |
Ocampo, Marisol Patarroyo, Manuel A. Vanegas, Magnolia Alba, Martha P. Patarroyo, Manuel E. |
author_sort |
Ocampo, Marisol |
title |
Functional, biochemical and 3D studies of Mycobacterium tuberculosis protein peptides for an effective anti-tuberculosis vaccine |
title_short |
Functional, biochemical and 3D studies of Mycobacterium tuberculosis protein peptides for an effective anti-tuberculosis vaccine |
title_full |
Functional, biochemical and 3D studies of Mycobacterium tuberculosis protein peptides for an effective anti-tuberculosis vaccine |
title_fullStr |
Functional, biochemical and 3D studies of Mycobacterium tuberculosis protein peptides for an effective anti-tuberculosis vaccine |
title_full_unstemmed |
Functional, biochemical and 3D studies of Mycobacterium tuberculosis protein peptides for an effective anti-tuberculosis vaccine |
title_sort |
functional, biochemical and 3d studies of mycobacterium tuberculosis protein peptides for an effective anti-tuberculosis vaccine |
publishDate |
2014 |
url |
https://repository.urosario.edu.co/handle/10336/23782 https://doi.org/10.3109/1040841X.2013.763221 |
_version_ |
1740173011007307776 |
score |
12,131701 |