Genomic analyses reveal moderate levels of ploidy, high heterozygosity and structural variations in a Colombian isolate of Leishmania (Leishmania) amazonensis

Leishmania amazonensis is one of the causative agents of the different forms of cutaneous leishmaniasis present in Latin America. This species has been isolated from humans and animals (canine/feline) in some endemic regions of Colombia. Therefore, L. amazonensis is of great relevance at the clinica...

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Autores Principales: Patino, Luz H., Muskus, Carlos, Muñoz, Marina, Ramírez, Juan David
Formato: Artículo (Article)
Lenguaje:Inglés (English)
Publicado: Elsevier B.V. 2020
Materias:
L
Acceso en línea:https://repository.urosario.edu.co/handle/10336/23719
https://doi.org/10.1016/j.actatropica.2019.105296
id ir-10336-23719
recordtype dspace
spelling ir-10336-237192022-05-02T12:37:18Z Genomic analyses reveal moderate levels of ploidy, high heterozygosity and structural variations in a Colombian isolate of Leishmania (Leishmania) amazonensis Patino, Luz H. Muskus, Carlos Muñoz, Marina Ramírez, Juan David Epidemiology Genetic variation Genomics Heterozygosity Leishmaniasis Parasite Ploidy Aneuploidy Article Chromosome variant Colombia Controlled study Copy number variation Genetic variation Genome analysis Heterozygosity Leishmania amazonensis Nonhuman Parasite development Parasite survival Parasite virulence Phylogeny Ploidy Single nucleotide polymorphism Species comparison Whole genome sequencing Colombia Animalia Leishmania amazonensis Aneuploidy Copy number variation Diversity Dna-seq Genome sequencing L Amazonensis Parasites Leishmania amazonensis is one of the causative agents of the different forms of cutaneous leishmaniasis present in Latin America. This species has been isolated from humans and animals (canine/feline) in some endemic regions of Colombia. Therefore, L. amazonensis is of great relevance at the clinical and epidemiological levels in medicine and veterinary science. Until now, very few genomes from this species are available. Here, we report the complete genome sequence of a laboratory-adapted L. amazonensis strain isolated from a human patient with clinical manifestation of cutaneous leishmaniasis in Colombia. The genome sequence not only allowed inter and intra-species comparative analyses to be performed with the sequenced genomes of L. amazonensis strains from different geographical regions, but also increased our knowledge about the genomic behavior of this L. amazonensis Colombian strain. This isolate was also characterized in terms of single nucleotide polymorphisms, chromosome and gene copy number variations (CNVs). The results revealed moderate aneuploidy, CNVs in genes involved in the virulence, growth, and survival of the parasite, and in the distributions of the multicopy genes on some chromosomes, as well as a high level of heterozygosity. The data confirmed previous reports that identified unique variations in L. amazonensis, suggesting aneuploidy may not have a high fitness cost and may allow the rapid generation of diversity in Leishmania parasites growing normally. © 2019 Elsevier B.V. 2020 2020-05-26T00:04:48Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 0001706X https://repository.urosario.edu.co/handle/10336/23719 https://doi.org/10.1016/j.actatropica.2019.105296 eng info:eu-repo/semantics/openAccess application/pdf Elsevier B.V. instname:Universidad del Rosario
institution EdocUR - Universidad del Rosario
collection DSpace
language Inglés (English)
topic Epidemiology
Genetic variation
Genomics
Heterozygosity
Leishmaniasis
Parasite
Ploidy
Aneuploidy
Article
Chromosome variant
Colombia
Controlled study
Copy number variation
Genetic variation
Genome analysis
Heterozygosity
Leishmania amazonensis
Nonhuman
Parasite development
Parasite survival
Parasite virulence
Phylogeny
Ploidy
Single nucleotide polymorphism
Species comparison
Whole genome sequencing
Colombia
Animalia
Leishmania amazonensis
Aneuploidy
Copy number variation
Diversity
Dna-seq
Genome sequencing
L
Amazonensis
Parasites
spellingShingle Epidemiology
Genetic variation
Genomics
Heterozygosity
Leishmaniasis
Parasite
Ploidy
Aneuploidy
Article
Chromosome variant
Colombia
Controlled study
Copy number variation
Genetic variation
Genome analysis
Heterozygosity
Leishmania amazonensis
Nonhuman
Parasite development
Parasite survival
Parasite virulence
Phylogeny
Ploidy
Single nucleotide polymorphism
Species comparison
Whole genome sequencing
Colombia
Animalia
Leishmania amazonensis
Aneuploidy
Copy number variation
Diversity
Dna-seq
Genome sequencing
L
Amazonensis
Parasites
Patino, Luz H.
Muskus, Carlos
Muñoz, Marina
Ramírez, Juan David
Genomic analyses reveal moderate levels of ploidy, high heterozygosity and structural variations in a Colombian isolate of Leishmania (Leishmania) amazonensis
description Leishmania amazonensis is one of the causative agents of the different forms of cutaneous leishmaniasis present in Latin America. This species has been isolated from humans and animals (canine/feline) in some endemic regions of Colombia. Therefore, L. amazonensis is of great relevance at the clinical and epidemiological levels in medicine and veterinary science. Until now, very few genomes from this species are available. Here, we report the complete genome sequence of a laboratory-adapted L. amazonensis strain isolated from a human patient with clinical manifestation of cutaneous leishmaniasis in Colombia. The genome sequence not only allowed inter and intra-species comparative analyses to be performed with the sequenced genomes of L. amazonensis strains from different geographical regions, but also increased our knowledge about the genomic behavior of this L. amazonensis Colombian strain. This isolate was also characterized in terms of single nucleotide polymorphisms, chromosome and gene copy number variations (CNVs). The results revealed moderate aneuploidy, CNVs in genes involved in the virulence, growth, and survival of the parasite, and in the distributions of the multicopy genes on some chromosomes, as well as a high level of heterozygosity. The data confirmed previous reports that identified unique variations in L. amazonensis, suggesting aneuploidy may not have a high fitness cost and may allow the rapid generation of diversity in Leishmania parasites growing normally. © 2019 Elsevier B.V.
format Artículo (Article)
author Patino, Luz H.
Muskus, Carlos
Muñoz, Marina
Ramírez, Juan David
author_facet Patino, Luz H.
Muskus, Carlos
Muñoz, Marina
Ramírez, Juan David
author_sort Patino, Luz H.
title Genomic analyses reveal moderate levels of ploidy, high heterozygosity and structural variations in a Colombian isolate of Leishmania (Leishmania) amazonensis
title_short Genomic analyses reveal moderate levels of ploidy, high heterozygosity and structural variations in a Colombian isolate of Leishmania (Leishmania) amazonensis
title_full Genomic analyses reveal moderate levels of ploidy, high heterozygosity and structural variations in a Colombian isolate of Leishmania (Leishmania) amazonensis
title_fullStr Genomic analyses reveal moderate levels of ploidy, high heterozygosity and structural variations in a Colombian isolate of Leishmania (Leishmania) amazonensis
title_full_unstemmed Genomic analyses reveal moderate levels of ploidy, high heterozygosity and structural variations in a Colombian isolate of Leishmania (Leishmania) amazonensis
title_sort genomic analyses reveal moderate levels of ploidy, high heterozygosity and structural variations in a colombian isolate of leishmania (leishmania) amazonensis
publisher Elsevier B.V.
publishDate 2020
url https://repository.urosario.edu.co/handle/10336/23719
https://doi.org/10.1016/j.actatropica.2019.105296
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score 12,131701