Specific interaction between mycobacterium tuberculosis lipoprotein-derived peptides and target cells inhibits Mycobacterial entry in vitro
Tuberculosis (TB) continues being one of the diseases having the greatest mortality rates around the world, 8.7 million cases having been reported in 2011. An efficient vaccine against TB having a great impact on public health is an urgent need. Usually, selecting antigens for vaccines has been base...
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| Formato: | Artículo (Article) |
| Lenguaje: | Inglés (English) |
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Blackwell Publishing Ltd
2014
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| Acceso en línea: | https://repository.urosario.edu.co/handle/10336/23650 https://doi.org/10.1111/cbdd.12365 |
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ir-10336-236502022-05-02T12:37:21Z Specific interaction between mycobacterium tuberculosis lipoprotein-derived peptides and target cells inhibits Mycobacterial entry in vitro Ocampo, Marisol Curtidor, Hernando Vanegas, Magnolia Patarroyo, Manuel A. Patarroyo, Manuel E. BCG vaccine Lipoprotein Mycobacterium tuberculosis lipoprotein Rv1411c Mycobacterium tuberculosis lipoprotein Rv1911c Mycobacterium tuberculosis lipoprotein Rv2270 Mycobacterium tuberculosis lipoprotein Rv3763 Unclassified drug Antibody Bacterial protein Lipoprotein Microsphere Peptide Protein binding A549 cell line Amino acid sequence Article Bacterial membrane Bacterial virulence Controlled study Epithelium cell Host pathogen interaction Human In vitro study Mycobacterium tuberculosis Nonhuman Protein expression Protein localization Protein structure Target cell U937 cell line Biology Chemistry Host pathogen interaction Immunology Metabolism Molecular genetics Mycobacterium tuberculosis Protein secondary structure Synthesis Tumor cell line Mycobacterium tuberculosis Amino Acid Sequence Antibodies Bacterial Proteins Computational Biology Host-Pathogen Interactions Humans Lipoproteins Microspheres Molecular Sequence Data Mycobacterium tuberculosis Peptides Protein Binding U937 Cells Antituberculosis vaccine High activity binding peptide Multi-epitope vaccine Mycobacterium tuberculosis Synthetic peptide Tumor Secondary Cell Line Protein Structure Tuberculosis (TB) continues being one of the diseases having the greatest mortality rates around the world, 8.7 million cases having been reported in 2011. An efficient vaccine against TB having a great impact on public health is an urgent need. Usually, selecting antigens for vaccines has been based on proteins having immunogenic properties for patients suffering TB and having had promising results in mice and non-human primates. Our approach has been based on a functional approach involving the pathogen-host interaction in the search for antigens to be included in designing an efficient, minimal, subunit-based anti-TB vaccine. This means that Mycobacterium tuberculosis has mainly been involved in studies and that lipoproteins represent an important kind of protein on the cell envelope which can also contribute towards this pathogen's virulence. This study has assessed the expression of four lipoproteins from M. tuberculosis H37Rv, that is, Rv1411c (LprG), Rv1911c (LppC), Rv2270 (LppN) and Rv3763 (LpqH), and the possible biological activity of peptides derived from these. Five peptides were found for these proteins which had high specific binding to both alveolar A549 epithelial cells and U937 monocyte-derived macrophages which were able to significantly inhibit mycobacterial entry to these cells in vitro. © 2014 John Wiley and Sons A/S. 2014 2020-05-26T00:04:02Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 17470277 https://repository.urosario.edu.co/handle/10336/23650 https://doi.org/10.1111/cbdd.12365 eng info:eu-repo/semantics/openAccess application/pdf Blackwell Publishing Ltd instname:Universidad del Rosario |
| institution |
EdocUR - Universidad del Rosario |
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DSpace |
| language |
Inglés (English) |
| topic |
BCG vaccine Lipoprotein Mycobacterium tuberculosis lipoprotein Rv1411c Mycobacterium tuberculosis lipoprotein Rv1911c Mycobacterium tuberculosis lipoprotein Rv2270 Mycobacterium tuberculosis lipoprotein Rv3763 Unclassified drug Antibody Bacterial protein Lipoprotein Microsphere Peptide Protein binding A549 cell line Amino acid sequence Article Bacterial membrane Bacterial virulence Controlled study Epithelium cell Host pathogen interaction Human In vitro study Mycobacterium tuberculosis Nonhuman Protein expression Protein localization Protein structure Target cell U937 cell line Biology Chemistry Host pathogen interaction Immunology Metabolism Molecular genetics Mycobacterium tuberculosis Protein secondary structure Synthesis Tumor cell line Mycobacterium tuberculosis Amino Acid Sequence Antibodies Bacterial Proteins Computational Biology Host-Pathogen Interactions Humans Lipoproteins Microspheres Molecular Sequence Data Mycobacterium tuberculosis Peptides Protein Binding U937 Cells Antituberculosis vaccine High activity binding peptide Multi-epitope vaccine Mycobacterium tuberculosis Synthetic peptide Tumor Secondary Cell Line Protein Structure |
| spellingShingle |
BCG vaccine Lipoprotein Mycobacterium tuberculosis lipoprotein Rv1411c Mycobacterium tuberculosis lipoprotein Rv1911c Mycobacterium tuberculosis lipoprotein Rv2270 Mycobacterium tuberculosis lipoprotein Rv3763 Unclassified drug Antibody Bacterial protein Lipoprotein Microsphere Peptide Protein binding A549 cell line Amino acid sequence Article Bacterial membrane Bacterial virulence Controlled study Epithelium cell Host pathogen interaction Human In vitro study Mycobacterium tuberculosis Nonhuman Protein expression Protein localization Protein structure Target cell U937 cell line Biology Chemistry Host pathogen interaction Immunology Metabolism Molecular genetics Mycobacterium tuberculosis Protein secondary structure Synthesis Tumor cell line Mycobacterium tuberculosis Amino Acid Sequence Antibodies Bacterial Proteins Computational Biology Host-Pathogen Interactions Humans Lipoproteins Microspheres Molecular Sequence Data Mycobacterium tuberculosis Peptides Protein Binding U937 Cells Antituberculosis vaccine High activity binding peptide Multi-epitope vaccine Mycobacterium tuberculosis Synthetic peptide Tumor Secondary Cell Line Protein Structure Ocampo, Marisol Curtidor, Hernando Vanegas, Magnolia Patarroyo, Manuel A. Patarroyo, Manuel E. Specific interaction between mycobacterium tuberculosis lipoprotein-derived peptides and target cells inhibits Mycobacterial entry in vitro |
| description |
Tuberculosis (TB) continues being one of the diseases having the greatest mortality rates around the world, 8.7 million cases having been reported in 2011. An efficient vaccine against TB having a great impact on public health is an urgent need. Usually, selecting antigens for vaccines has been based on proteins having immunogenic properties for patients suffering TB and having had promising results in mice and non-human primates. Our approach has been based on a functional approach involving the pathogen-host interaction in the search for antigens to be included in designing an efficient, minimal, subunit-based anti-TB vaccine. This means that Mycobacterium tuberculosis has mainly been involved in studies and that lipoproteins represent an important kind of protein on the cell envelope which can also contribute towards this pathogen's virulence. This study has assessed the expression of four lipoproteins from M. tuberculosis H37Rv, that is, Rv1411c (LprG), Rv1911c (LppC), Rv2270 (LppN) and Rv3763 (LpqH), and the possible biological activity of peptides derived from these. Five peptides were found for these proteins which had high specific binding to both alveolar A549 epithelial cells and U937 monocyte-derived macrophages which were able to significantly inhibit mycobacterial entry to these cells in vitro. © 2014 John Wiley and Sons A/S. |
| format |
Artículo (Article) |
| author |
Ocampo, Marisol Curtidor, Hernando Vanegas, Magnolia Patarroyo, Manuel A. Patarroyo, Manuel E. |
| author_facet |
Ocampo, Marisol Curtidor, Hernando Vanegas, Magnolia Patarroyo, Manuel A. Patarroyo, Manuel E. |
| author_sort |
Ocampo, Marisol |
| title |
Specific interaction between mycobacterium tuberculosis lipoprotein-derived peptides and target cells inhibits Mycobacterial entry in vitro |
| title_short |
Specific interaction between mycobacterium tuberculosis lipoprotein-derived peptides and target cells inhibits Mycobacterial entry in vitro |
| title_full |
Specific interaction between mycobacterium tuberculosis lipoprotein-derived peptides and target cells inhibits Mycobacterial entry in vitro |
| title_fullStr |
Specific interaction between mycobacterium tuberculosis lipoprotein-derived peptides and target cells inhibits Mycobacterial entry in vitro |
| title_full_unstemmed |
Specific interaction between mycobacterium tuberculosis lipoprotein-derived peptides and target cells inhibits Mycobacterial entry in vitro |
| title_sort |
specific interaction between mycobacterium tuberculosis lipoprotein-derived peptides and target cells inhibits mycobacterial entry in vitro |
| publisher |
Blackwell Publishing Ltd |
| publishDate |
2014 |
| url |
https://repository.urosario.edu.co/handle/10336/23650 https://doi.org/10.1111/cbdd.12365 |
| _version_ |
1740172884999929856 |
| score |
12,131701 |