Molecular and descriptive epidemiology of intestinal protozoan parasites of children and their pets in Cauca, Colombia: A cross-sectional study

Background: Parasitic infections, particularly those caused by protozoa, represent a considerable public health problem in developing countries. Blastocystis, Giardia duodenalis, Cryptosporidium spp. and the Entamoeba complex (Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii) are th...

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Autores Principales: Villamizar, Ximena, Higuera, Adriana, Herrera, Giovanny, Vasquez-A, Luis Reinel, Buitron, Lorena, Muñoz, Lina Maria, Gonzalez-C, Fabiola E., Lopez, Myriam Consuelo, Giraldo, Julio Cesar, Ramírez, Juan David
Formato: Artículo (Article)
Lenguaje:Inglés (English)
Publicado: BioMed Central Ltd. 2019
Materias:
Dog
Acceso en línea:https://repository.urosario.edu.co/handle/10336/23568
https://doi.org/10.1186/s12879-019-3810-0
id ir-10336-23568
recordtype dspace
institution EdocUR - Universidad del Rosario
collection DSpace
language Inglés (English)
topic Allele
Article
Blastocystis
Child
Chilomastix
Colombia
Controlled study
Cross-sectional study
Cryptococcus neoformans
Cryptosporidium parvum
Demography
Diagnostic test accuracy study
Diagnostic value
Dog
Domestic animal
Endolimax nana
Entamoeba histolytica
Escherichia coli
Feces analysis
Female
Giardia intestinalis
Human
Intermethod comparison
Intestine infection
Intestine parasite
Major clinical study
Male
Microscopy
Molecular epidemiology
Nonhuman
Parasite identification
Parasite prevalence
Parasite transmission
Parasitism
Parasitosis
Polymerase chain reaction
Protozoon
Real time polymerase chain reaction
Amebiasis
Animal
Blastocystosis
Comparative study
Cryptosporidiosis
Dog
Feces
Giardiasis
Intestine infection
Molecular epidemiology
Parasitology
Pet animal
Preschool child
Prevalence
Procedures
Socioeconomics
Veterinary medicine
Animals
Blastocystis infections
Colombia
Cross-sectional studies
Cryptosporidiosis
Dogs
Entamoebiasis
Feces
Female
Giardiasis
Humans
Male
Microscopy
Molecular epidemiology
Pets
Prevalence
Real-time polymerase chain reaction
Socioeconomic factors
Blastocystis
Cryptosporidium
Entamoeba histolytica/dispar/moshkovskii complex
Giardia duodenalis
Zoonotic disease
preschool
parasitic
Child
Intestinal diseases
spellingShingle Allele
Article
Blastocystis
Child
Chilomastix
Colombia
Controlled study
Cross-sectional study
Cryptococcus neoformans
Cryptosporidium parvum
Demography
Diagnostic test accuracy study
Diagnostic value
Dog
Domestic animal
Endolimax nana
Entamoeba histolytica
Escherichia coli
Feces analysis
Female
Giardia intestinalis
Human
Intermethod comparison
Intestine infection
Intestine parasite
Major clinical study
Male
Microscopy
Molecular epidemiology
Nonhuman
Parasite identification
Parasite prevalence
Parasite transmission
Parasitism
Parasitosis
Polymerase chain reaction
Protozoon
Real time polymerase chain reaction
Amebiasis
Animal
Blastocystosis
Comparative study
Cryptosporidiosis
Dog
Feces
Giardiasis
Intestine infection
Molecular epidemiology
Parasitology
Pet animal
Preschool child
Prevalence
Procedures
Socioeconomics
Veterinary medicine
Animals
Blastocystis infections
Colombia
Cross-sectional studies
Cryptosporidiosis
Dogs
Entamoebiasis
Feces
Female
Giardiasis
Humans
Male
Microscopy
Molecular epidemiology
Pets
Prevalence
Real-time polymerase chain reaction
Socioeconomic factors
Blastocystis
Cryptosporidium
Entamoeba histolytica/dispar/moshkovskii complex
Giardia duodenalis
Zoonotic disease
preschool
parasitic
Child
Intestinal diseases
Villamizar, Ximena
Higuera, Adriana
Herrera, Giovanny
Vasquez-A, Luis Reinel
Buitron, Lorena
Muñoz, Lina Maria
Gonzalez-C, Fabiola E.
Lopez, Myriam Consuelo
Giraldo, Julio Cesar
Ramírez, Juan David
Molecular and descriptive epidemiology of intestinal protozoan parasites of children and their pets in Cauca, Colombia: A cross-sectional study
description Background: Parasitic infections, particularly those caused by protozoa, represent a considerable public health problem in developing countries. Blastocystis, Giardia duodenalis, Cryptosporidium spp. and the Entamoeba complex (Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii) are the most common etiological causes of intestinal parasitic infections. Methods: We carried out a descriptive cross-sectional study in school-age children attending a daycare institution in commune eight of Popayán, Cauca (Southwest Colombia). A total of 266 fecal samples were collected (258 from children and eight from pets). Blastocystis, G. duodenalis, Cryptosporidium spp. and the Entamoeba complex were identified by microscopy, quantitative real-time PCR (qPCR) and conventional PCR. The concordance of qPCR and microscopy was assessed using the Kappa index. Molecular characterization was conducted to identify Blastocystis subtypes (18S), G. duodenalis assemblages (tpi and gdh) and Cryptosporidium species/subtypes (18S and GP60). Potential associations between intestinal parasitism and sociodemographic factors were examined using bivariate analyses. Results: A total of 258 fecal samples from children were analyzed by microscopy and 255 samples were analyzed by qPCR. The prevalence of Blastocystis was between 25.19% (microscopy) and 39.22% (qPCR), that of G. duodenalis was between 8.14% (microscopy) and 10.59% (qPCR), that of Cryptosporidium spp. was estimated at 9.8% (qPCR), and that of the Entamoeba complex was between 0.39% (conventional PCR) and 0.78% (microscopy). The concordance between microscopy and qPCR was very low. Blastocystis ST1 (alleles 4, 8, and 80), ST2 (alleles 11, 12, and 15), ST3 (alleles 31, 34, 36, 38,57, and 151), and ST4 (alleles 42 and 91), G. duodenalis assemblages AII, BIII, BIV and D, C. parvum subtype IIa and C. hominis subtype IbA9G3R2 were identified. The only identified member of the Entamoeba complex corresponded to E. histolytica. No statistically significant association was identified between parasitic infection and any sociodemographic variable. Conclusion: This study revealed the usefulness of molecular methods to depict the transmission dynamics of parasitic protozoa in southwest Colombia. The presence of some of these protozoa in domestic animals may be involved in their transmission. © 2019 The Author(s).
format Artículo (Article)
author Villamizar, Ximena
Higuera, Adriana
Herrera, Giovanny
Vasquez-A, Luis Reinel
Buitron, Lorena
Muñoz, Lina Maria
Gonzalez-C, Fabiola E.
Lopez, Myriam Consuelo
Giraldo, Julio Cesar
Ramírez, Juan David
author_facet Villamizar, Ximena
Higuera, Adriana
Herrera, Giovanny
Vasquez-A, Luis Reinel
Buitron, Lorena
Muñoz, Lina Maria
Gonzalez-C, Fabiola E.
Lopez, Myriam Consuelo
Giraldo, Julio Cesar
Ramírez, Juan David
author_sort Villamizar, Ximena
title Molecular and descriptive epidemiology of intestinal protozoan parasites of children and their pets in Cauca, Colombia: A cross-sectional study
title_short Molecular and descriptive epidemiology of intestinal protozoan parasites of children and their pets in Cauca, Colombia: A cross-sectional study
title_full Molecular and descriptive epidemiology of intestinal protozoan parasites of children and their pets in Cauca, Colombia: A cross-sectional study
title_fullStr Molecular and descriptive epidemiology of intestinal protozoan parasites of children and their pets in Cauca, Colombia: A cross-sectional study
title_full_unstemmed Molecular and descriptive epidemiology of intestinal protozoan parasites of children and their pets in Cauca, Colombia: A cross-sectional study
title_sort molecular and descriptive epidemiology of intestinal protozoan parasites of children and their pets in cauca, colombia: a cross-sectional study
publisher BioMed Central Ltd.
publishDate 2019
url https://repository.urosario.edu.co/handle/10336/23568
https://doi.org/10.1186/s12879-019-3810-0
_version_ 1740172073333948416
spelling ir-10336-235682022-05-02T12:37:18Z Molecular and descriptive epidemiology of intestinal protozoan parasites of children and their pets in Cauca, Colombia: A cross-sectional study Villamizar, Ximena Higuera, Adriana Herrera, Giovanny Vasquez-A, Luis Reinel Buitron, Lorena Muñoz, Lina Maria Gonzalez-C, Fabiola E. Lopez, Myriam Consuelo Giraldo, Julio Cesar Ramírez, Juan David Allele Article Blastocystis Child Chilomastix Colombia Controlled study Cross-sectional study Cryptococcus neoformans Cryptosporidium parvum Demography Diagnostic test accuracy study Diagnostic value Dog Domestic animal Endolimax nana Entamoeba histolytica Escherichia coli Feces analysis Female Giardia intestinalis Human Intermethod comparison Intestine infection Intestine parasite Major clinical study Male Microscopy Molecular epidemiology Nonhuman Parasite identification Parasite prevalence Parasite transmission Parasitism Parasitosis Polymerase chain reaction Protozoon Real time polymerase chain reaction Amebiasis Animal Blastocystosis Comparative study Cryptosporidiosis Dog Feces Giardiasis Intestine infection Molecular epidemiology Parasitology Pet animal Preschool child Prevalence Procedures Socioeconomics Veterinary medicine Animals Blastocystis infections Colombia Cross-sectional studies Cryptosporidiosis Dogs Entamoebiasis Feces Female Giardiasis Humans Male Microscopy Molecular epidemiology Pets Prevalence Real-time polymerase chain reaction Socioeconomic factors Blastocystis Cryptosporidium Entamoeba histolytica/dispar/moshkovskii complex Giardia duodenalis Zoonotic disease preschool parasitic Child Intestinal diseases Background: Parasitic infections, particularly those caused by protozoa, represent a considerable public health problem in developing countries. Blastocystis, Giardia duodenalis, Cryptosporidium spp. and the Entamoeba complex (Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii) are the most common etiological causes of intestinal parasitic infections. Methods: We carried out a descriptive cross-sectional study in school-age children attending a daycare institution in commune eight of Popayán, Cauca (Southwest Colombia). A total of 266 fecal samples were collected (258 from children and eight from pets). Blastocystis, G. duodenalis, Cryptosporidium spp. and the Entamoeba complex were identified by microscopy, quantitative real-time PCR (qPCR) and conventional PCR. The concordance of qPCR and microscopy was assessed using the Kappa index. Molecular characterization was conducted to identify Blastocystis subtypes (18S), G. duodenalis assemblages (tpi and gdh) and Cryptosporidium species/subtypes (18S and GP60). Potential associations between intestinal parasitism and sociodemographic factors were examined using bivariate analyses. Results: A total of 258 fecal samples from children were analyzed by microscopy and 255 samples were analyzed by qPCR. The prevalence of Blastocystis was between 25.19% (microscopy) and 39.22% (qPCR), that of G. duodenalis was between 8.14% (microscopy) and 10.59% (qPCR), that of Cryptosporidium spp. was estimated at 9.8% (qPCR), and that of the Entamoeba complex was between 0.39% (conventional PCR) and 0.78% (microscopy). The concordance between microscopy and qPCR was very low. Blastocystis ST1 (alleles 4, 8, and 80), ST2 (alleles 11, 12, and 15), ST3 (alleles 31, 34, 36, 38,57, and 151), and ST4 (alleles 42 and 91), G. duodenalis assemblages AII, BIII, BIV and D, C. parvum subtype IIa and C. hominis subtype IbA9G3R2 were identified. The only identified member of the Entamoeba complex corresponded to E. histolytica. No statistically significant association was identified between parasitic infection and any sociodemographic variable. Conclusion: This study revealed the usefulness of molecular methods to depict the transmission dynamics of parasitic protozoa in southwest Colombia. The presence of some of these protozoa in domestic animals may be involved in their transmission. © 2019 The Author(s). 2019 2020-05-26T00:03:11Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 14712334 https://repository.urosario.edu.co/handle/10336/23568 https://doi.org/10.1186/s12879-019-3810-0 eng info:eu-repo/semantics/openAccess application/pdf BioMed Central Ltd. instname:Universidad del Rosario
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