Evaluation of four rapid diagnostic tests for canine and human visceral Leishmaniasis in Colombia

Background: Leishmaniasis caused by different species of Leishmania affect 98 countries worldwide. Visceral Leishmaniasis (VL) is the mortal clinical presentation of the disease that causes the dead to more than 90% of the patients who suffer it. The diagnosis of VL is made by the direct observation...

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Autores Principales: Herrera, Giovanny, Castillo, Adriana, Ayala, Martha S., Flórez, Carolina, Cantillo-Barraza, Omar, Ramírez, Juan David
Formato: Artículo (Article)
Lenguaje:Inglés (English)
Publicado: BioMed Central Ltd. 2019
Materias:
Dog
Pcr
Acceso en línea:https://repository.urosario.edu.co/handle/10336/23567
https://doi.org/10.1186/s12879-019-4353-0
id ir-10336-23567
recordtype dspace
spelling ir-10336-235672022-05-02T12:37:21Z Evaluation of four rapid diagnostic tests for canine and human visceral Leishmaniasis in Colombia Herrera, Giovanny Castillo, Adriana Ayala, Martha S. Flórez, Carolina Cantillo-Barraza, Omar Ramírez, Juan David Heat shock protein 70 Article Clinical effectiveness Colombia Dog breed Human Immunofluorescence Major clinical study Nonhuman Polymerase chain reaction Predictive value Receiver operating characteristic Sanger sequencing Sensitivity and specificity Visceral leishmaniasis Animal Diagnostic test Dog Dog disease Evaluation study Genetics Immunoassay Immunology Indirect fluorescent antibody technique Leishmania braziliensis Leishmania infantum Parasitology Procedures Veterinary medicine Visceral leishmaniasis Animals Colombia Dog diseases Dogs Humans Immunoassay Leishmania braziliensis Leishmania infantum Polymerase chain reaction Sensitivity and specificity Immunochromatographic test Indirect immunofluorescence Pcr Sensitivity Specificity Visceral leishmaniasis indirect routine visceral Diagnostic tests Fluorescent antibody technique Leishmaniasis Background: Leishmaniasis caused by different species of Leishmania affect 98 countries worldwide. Visceral Leishmaniasis (VL) is the mortal clinical presentation of the disease that causes the dead to more than 90% of the patients who suffer it. The diagnosis of VL is made by the direct observation of the parasite in bone marrow, spleen and/or liver aspirates that requires complex proceedings. Therefore, serum samples are submitted to Indirect Immunofluorescence to identify the presence of anti-Leishmania antibodies. Despite the variability in the diagnostic performance of the Immunochromatographic Tests (ICTs), there are many evidences that suggest that ICTs can be used for epidemiological screening. However, in Colombia there are not any evidence about the performance of the ICTs for VL diagnosis, both for human and canine serum samples. Therefore, this study evaluated the diagnostic performance of 4 ICTs for VL (2 ICTs in human sera and 2 ICTs in canine sera) in samples from endemic areas of Colombia. Methods: We selected a total of 156 human serum samples (82 positive and 74 negative for VL) and 126 canine serum samples (71 positive and 54 negative) diagnosed by in house Indirect Immunofluorescence (IIF). The samples were submitted to the ICTs following the manufacturers' instructions. Statistical analysis was performed to evaluate the diagnostic performance of each ICT in comparison with the IIF. PCR for HSP70 gene and sanger sequencing was performed in samples with negative results for both ICTs. Results: The sensitivity (S) of both ICTs for human samples (Ad-bio Leishmania IgG/IgM Combo Rapid Test and Kalazar Detect™) was 91.5% and specificity (E) were 93.2 and 89.2% respectively, while for the ICTs tested on canine samples (Kalazar Detect™ Rapid Test, Canine and DPP® CVL rapid test) we found S values between 82.9 and 85.7% and E values between 79.6 and 92.6%. We found L. infantum by PCR and sequencing in 2 human samples, and L. braziliensis and L. amazonensis in canine serum samples that were negative by both ICTs. Conclusions: We conclude that both tests evaluated on human samples have a similar diagnostic performance, while the Kalazar Detect™ Rapid Test, Canine showed a better diagnostic performance than the DPP® CVL rapid test evaluated on canine samples. Also, we suggest that it is necessary to design tests with antigens of the circulating strains to increase its diagnostic utility. © 2019 The Author(s). 2019 2020-05-26T00:03:10Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 14712334 https://repository.urosario.edu.co/handle/10336/23567 https://doi.org/10.1186/s12879-019-4353-0 eng info:eu-repo/semantics/openAccess application/pdf BioMed Central Ltd. instname:Universidad del Rosario
institution EdocUR - Universidad del Rosario
collection DSpace
language Inglés (English)
topic Heat shock protein 70
Article
Clinical effectiveness
Colombia
Dog breed
Human
Immunofluorescence
Major clinical study
Nonhuman
Polymerase chain reaction
Predictive value
Receiver operating characteristic
Sanger sequencing
Sensitivity and specificity
Visceral leishmaniasis
Animal
Diagnostic test
Dog
Dog disease
Evaluation study
Genetics
Immunoassay
Immunology
Indirect fluorescent antibody technique
Leishmania braziliensis
Leishmania infantum
Parasitology
Procedures
Veterinary medicine
Visceral leishmaniasis
Animals
Colombia
Dog diseases
Dogs
Humans
Immunoassay
Leishmania braziliensis
Leishmania infantum
Polymerase chain reaction
Sensitivity and specificity
Immunochromatographic test
Indirect immunofluorescence
Pcr
Sensitivity
Specificity
Visceral leishmaniasis
indirect
routine
visceral
Diagnostic tests
Fluorescent antibody technique
Leishmaniasis
spellingShingle Heat shock protein 70
Article
Clinical effectiveness
Colombia
Dog breed
Human
Immunofluorescence
Major clinical study
Nonhuman
Polymerase chain reaction
Predictive value
Receiver operating characteristic
Sanger sequencing
Sensitivity and specificity
Visceral leishmaniasis
Animal
Diagnostic test
Dog
Dog disease
Evaluation study
Genetics
Immunoassay
Immunology
Indirect fluorescent antibody technique
Leishmania braziliensis
Leishmania infantum
Parasitology
Procedures
Veterinary medicine
Visceral leishmaniasis
Animals
Colombia
Dog diseases
Dogs
Humans
Immunoassay
Leishmania braziliensis
Leishmania infantum
Polymerase chain reaction
Sensitivity and specificity
Immunochromatographic test
Indirect immunofluorescence
Pcr
Sensitivity
Specificity
Visceral leishmaniasis
indirect
routine
visceral
Diagnostic tests
Fluorescent antibody technique
Leishmaniasis
Herrera, Giovanny
Castillo, Adriana
Ayala, Martha S.
Flórez, Carolina
Cantillo-Barraza, Omar
Ramírez, Juan David
Evaluation of four rapid diagnostic tests for canine and human visceral Leishmaniasis in Colombia
description Background: Leishmaniasis caused by different species of Leishmania affect 98 countries worldwide. Visceral Leishmaniasis (VL) is the mortal clinical presentation of the disease that causes the dead to more than 90% of the patients who suffer it. The diagnosis of VL is made by the direct observation of the parasite in bone marrow, spleen and/or liver aspirates that requires complex proceedings. Therefore, serum samples are submitted to Indirect Immunofluorescence to identify the presence of anti-Leishmania antibodies. Despite the variability in the diagnostic performance of the Immunochromatographic Tests (ICTs), there are many evidences that suggest that ICTs can be used for epidemiological screening. However, in Colombia there are not any evidence about the performance of the ICTs for VL diagnosis, both for human and canine serum samples. Therefore, this study evaluated the diagnostic performance of 4 ICTs for VL (2 ICTs in human sera and 2 ICTs in canine sera) in samples from endemic areas of Colombia. Methods: We selected a total of 156 human serum samples (82 positive and 74 negative for VL) and 126 canine serum samples (71 positive and 54 negative) diagnosed by in house Indirect Immunofluorescence (IIF). The samples were submitted to the ICTs following the manufacturers' instructions. Statistical analysis was performed to evaluate the diagnostic performance of each ICT in comparison with the IIF. PCR for HSP70 gene and sanger sequencing was performed in samples with negative results for both ICTs. Results: The sensitivity (S) of both ICTs for human samples (Ad-bio Leishmania IgG/IgM Combo Rapid Test and Kalazar Detect™) was 91.5% and specificity (E) were 93.2 and 89.2% respectively, while for the ICTs tested on canine samples (Kalazar Detect™ Rapid Test, Canine and DPP® CVL rapid test) we found S values between 82.9 and 85.7% and E values between 79.6 and 92.6%. We found L. infantum by PCR and sequencing in 2 human samples, and L. braziliensis and L. amazonensis in canine serum samples that were negative by both ICTs. Conclusions: We conclude that both tests evaluated on human samples have a similar diagnostic performance, while the Kalazar Detect™ Rapid Test, Canine showed a better diagnostic performance than the DPP® CVL rapid test evaluated on canine samples. Also, we suggest that it is necessary to design tests with antigens of the circulating strains to increase its diagnostic utility. © 2019 The Author(s).
format Artículo (Article)
author Herrera, Giovanny
Castillo, Adriana
Ayala, Martha S.
Flórez, Carolina
Cantillo-Barraza, Omar
Ramírez, Juan David
author_facet Herrera, Giovanny
Castillo, Adriana
Ayala, Martha S.
Flórez, Carolina
Cantillo-Barraza, Omar
Ramírez, Juan David
author_sort Herrera, Giovanny
title Evaluation of four rapid diagnostic tests for canine and human visceral Leishmaniasis in Colombia
title_short Evaluation of four rapid diagnostic tests for canine and human visceral Leishmaniasis in Colombia
title_full Evaluation of four rapid diagnostic tests for canine and human visceral Leishmaniasis in Colombia
title_fullStr Evaluation of four rapid diagnostic tests for canine and human visceral Leishmaniasis in Colombia
title_full_unstemmed Evaluation of four rapid diagnostic tests for canine and human visceral Leishmaniasis in Colombia
title_sort evaluation of four rapid diagnostic tests for canine and human visceral leishmaniasis in colombia
publisher BioMed Central Ltd.
publishDate 2019
url https://repository.urosario.edu.co/handle/10336/23567
https://doi.org/10.1186/s12879-019-4353-0
_version_ 1740172971641667584
score 12,131701