Specific Binding Peptides from Rv3632: A Strategy for Blocking Mycobacterium tuberculosis Entry to Target Cells?

Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis (Mtb, i.e., the aetiological agent); the WHO has established this disease as high priority due to its ensuing mortality. Mtb uses a range of mechanisms for preventing its elimination by an infected host; new, viable alternati...

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Autores Principales: Sánchez-Barinas, Christian David, Tabares, Luisa, Bermúdez, Maritza, Patarroyo, Manuel Elkin, Ocampo, Marisol, Patarroyo, Manuel A.
Formato: Artículo (Article)
Lenguaje:Inglés (English)
Publicado: Hindawi Limited 2019
Materias:
Acceso en línea:https://repository.urosario.edu.co/handle/10336/23528
https://doi.org/10.1155/2019/8680935
id ir-10336-23528
recordtype dspace
spelling ir-10336-235282022-05-02T12:37:14Z Specific Binding Peptides from Rv3632: A Strategy for Blocking Mycobacterium tuberculosis Entry to Target Cells? Sánchez-Barinas, Christian David Tabares, Luisa Bermúdez, Maritza Patarroyo, Manuel Elkin Ocampo, Marisol Patarroyo, Manuel A. Arginine Epitope Glycine Peptide Synthetic peptide Threonine Bacterial protein Peptide Protein binding A-549 cell line Animal cell Article Bacterial membrane Bioinformatics Carboxy terminal sequence Circular dichroism Concentration response Female Host pathogen interaction Latent tuberculosis Lung alveolus epithelium cell Mortality Mouse Mycobacterium tuberculosis Nonhuman Protein secondary structure Target cell U-937 cell line Amino acid sequence Biology Cell membrane Cell wall Chemistry Genetic transcription Genetics Human Isolation and purification Metabolism Molecular model Mycobacterium tuberculosis A549 cells Amino acid sequence Bacterial proteins Cell membrane Cell wall Circular dichroism Computational biology Host-pathogen interactions Humans Mycobacterium tuberculosis Peptides Protein binding U937 cells genetic secondary molecular Models Protein structure Transcription Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis (Mtb, i.e., the aetiological agent); the WHO has established this disease as high priority due to its ensuing mortality. Mtb uses a range of mechanisms for preventing its elimination by an infected host; new, viable alternatives for blocking the host-pathogen interaction are thus sought constantly. This article updates our laboratory's systematic search for antigens using bioinformatics tools to clarify the Mtb H37Rv Rv3632 protein's topology and location. This article reports a C-terminal region consisting of peptides 39255 and 39256 (81Thr-Arg114) having high specific binding regarding two infection-related cell lines (A549 and U937); they inhibited mycobacterial entry to U937 cells in a concentration-dependent manner. Rv3632 forms part of the mycobacterial cell envelope, formed by six linear synthetic peptides. Circular dichroism enabled determining the protein's secondary structure. It was also found that peptide 39254 (61Gly-Thr83) was a HABP for alveolar epithelial cells and inhibited mycobacteria entry to these cells regardless of concentration. Sera from active or latent tuberculosis patients did not recognise HABPs 39254 and 39256. These sequences represent a promising approach aiming at their ongoing modification and for including them when designing a multi-epitope, anti-tuberculosis vaccine. © 2019 Christian David Sánchez-Barinas et al. 2019 2020-05-26T00:02:49Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 23146141 23146133 https://repository.urosario.edu.co/handle/10336/23528 https://doi.org/10.1155/2019/8680935 eng info:eu-repo/semantics/openAccess application/pdf Hindawi Limited instname:Universidad del Rosario
institution EdocUR - Universidad del Rosario
collection DSpace
language Inglés (English)
topic Arginine
Epitope
Glycine
Peptide
Synthetic peptide
Threonine
Bacterial protein
Peptide
Protein binding
A-549 cell line
Animal cell
Article
Bacterial membrane
Bioinformatics
Carboxy terminal sequence
Circular dichroism
Concentration response
Female
Host pathogen interaction
Latent tuberculosis
Lung alveolus epithelium cell
Mortality
Mouse
Mycobacterium tuberculosis
Nonhuman
Protein secondary structure
Target cell
U-937 cell line
Amino acid sequence
Biology
Cell membrane
Cell wall
Chemistry
Genetic transcription
Genetics
Human
Isolation and purification
Metabolism
Molecular model
Mycobacterium tuberculosis
A549 cells
Amino acid sequence
Bacterial proteins
Cell membrane
Cell wall
Circular dichroism
Computational biology
Host-pathogen interactions
Humans
Mycobacterium tuberculosis
Peptides
Protein binding
U937 cells
genetic
secondary
molecular
Models
Protein structure
Transcription
spellingShingle Arginine
Epitope
Glycine
Peptide
Synthetic peptide
Threonine
Bacterial protein
Peptide
Protein binding
A-549 cell line
Animal cell
Article
Bacterial membrane
Bioinformatics
Carboxy terminal sequence
Circular dichroism
Concentration response
Female
Host pathogen interaction
Latent tuberculosis
Lung alveolus epithelium cell
Mortality
Mouse
Mycobacterium tuberculosis
Nonhuman
Protein secondary structure
Target cell
U-937 cell line
Amino acid sequence
Biology
Cell membrane
Cell wall
Chemistry
Genetic transcription
Genetics
Human
Isolation and purification
Metabolism
Molecular model
Mycobacterium tuberculosis
A549 cells
Amino acid sequence
Bacterial proteins
Cell membrane
Cell wall
Circular dichroism
Computational biology
Host-pathogen interactions
Humans
Mycobacterium tuberculosis
Peptides
Protein binding
U937 cells
genetic
secondary
molecular
Models
Protein structure
Transcription
Sánchez-Barinas, Christian David
Tabares, Luisa
Bermúdez, Maritza
Patarroyo, Manuel Elkin
Ocampo, Marisol
Patarroyo, Manuel A.
Specific Binding Peptides from Rv3632: A Strategy for Blocking Mycobacterium tuberculosis Entry to Target Cells?
description Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis (Mtb, i.e., the aetiological agent); the WHO has established this disease as high priority due to its ensuing mortality. Mtb uses a range of mechanisms for preventing its elimination by an infected host; new, viable alternatives for blocking the host-pathogen interaction are thus sought constantly. This article updates our laboratory's systematic search for antigens using bioinformatics tools to clarify the Mtb H37Rv Rv3632 protein's topology and location. This article reports a C-terminal region consisting of peptides 39255 and 39256 (81Thr-Arg114) having high specific binding regarding two infection-related cell lines (A549 and U937); they inhibited mycobacterial entry to U937 cells in a concentration-dependent manner. Rv3632 forms part of the mycobacterial cell envelope, formed by six linear synthetic peptides. Circular dichroism enabled determining the protein's secondary structure. It was also found that peptide 39254 (61Gly-Thr83) was a HABP for alveolar epithelial cells and inhibited mycobacteria entry to these cells regardless of concentration. Sera from active or latent tuberculosis patients did not recognise HABPs 39254 and 39256. These sequences represent a promising approach aiming at their ongoing modification and for including them when designing a multi-epitope, anti-tuberculosis vaccine. © 2019 Christian David Sánchez-Barinas et al.
format Artículo (Article)
author Sánchez-Barinas, Christian David
Tabares, Luisa
Bermúdez, Maritza
Patarroyo, Manuel Elkin
Ocampo, Marisol
Patarroyo, Manuel A.
author_facet Sánchez-Barinas, Christian David
Tabares, Luisa
Bermúdez, Maritza
Patarroyo, Manuel Elkin
Ocampo, Marisol
Patarroyo, Manuel A.
author_sort Sánchez-Barinas, Christian David
title Specific Binding Peptides from Rv3632: A Strategy for Blocking Mycobacterium tuberculosis Entry to Target Cells?
title_short Specific Binding Peptides from Rv3632: A Strategy for Blocking Mycobacterium tuberculosis Entry to Target Cells?
title_full Specific Binding Peptides from Rv3632: A Strategy for Blocking Mycobacterium tuberculosis Entry to Target Cells?
title_fullStr Specific Binding Peptides from Rv3632: A Strategy for Blocking Mycobacterium tuberculosis Entry to Target Cells?
title_full_unstemmed Specific Binding Peptides from Rv3632: A Strategy for Blocking Mycobacterium tuberculosis Entry to Target Cells?
title_sort specific binding peptides from rv3632: a strategy for blocking mycobacterium tuberculosis entry to target cells?
publisher Hindawi Limited
publishDate 2019
url https://repository.urosario.edu.co/handle/10336/23528
https://doi.org/10.1155/2019/8680935
_version_ 1740172656825597952
score 12,131701