The Plasmodium vivax Pv41 surface protein: Identification and characterization

Recently, Plasmodium vivax has been related to nearly 81% of malaria cases reported in Central America and the Mediterranean. Due to the difficulty of culturing this parasite species in vitro, most studies on P. vivax have focused on the identification of new antigens by homology comparison with P....

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Autores Principales: Angel, Diana I., Mongui, Alvaro, Vanegas, Magnolia, Patarroyo, Manuel A., Ardila-Montealegre, Javier
Formato: Artículo (Article)
Lenguaje:Inglés (English)
Publicado: 2008
Materias:
Acceso en línea:https://repository.urosario.edu.co/handle/10336/23503
https://doi.org/10.1016/j.bbrc.2008.10.129
id ir-10336-23503
recordtype dspace
spelling ir-10336-235032022-05-02T12:37:14Z The Plasmodium vivax Pv41 surface protein: Identification and characterization Angel, Diana I. Mongui, Alvaro Vanegas, Magnolia Patarroyo, Manuel A. Ardila-Montealegre, Javier Amino acid Antigen Membrane protein Protozoal protein Article Bioinformatics Cellular distribution Data base Exon Gametocyte Immunohistochemistry Life cycle Nonhuman Plasmodium vivax Priority journal Protein analysis Protein determination Protein domain Protein expression Amino acid sequence Animals Cell membrane Erythrocytes Exons Membrane proteins Plasmodium vivax Protozoan proteins Rabbits Plasmodium falciparum Plasmodium vivax Bioinformatics Malaria Plasmodium vivax Pv41 Six-cys domain Vaccine candidate molecular genetic tertiary protozoan Antigens Cloning Protein structure Transcription Recently, Plasmodium vivax has been related to nearly 81% of malaria cases reported in Central America and the Mediterranean. Due to the difficulty of culturing this parasite species in vitro, most studies on P. vivax have focused on the identification of new antigens by homology comparison with P. falciparum vaccine candidate proteins. In this study, we have identified and characterized a Pf41 homologue in P. vivax, hence named Pv41, by following such approach and using web-available bioinformatics databases, molecular techniques and immunochemistry assays. Pv41 protein is a 384-amino-acid-long antigen encoded by a single exon that exhibits two s48/45 domains characteristic of gametocyte surface proteins. We have also demonstrated Pv41 transcription and expression during late intra-erythrocytic parasite stages and defined its subcellular localization on the parasite surface. © 2008 Elsevier Inc. All rights reserved. 2008 2020-05-26T00:02:34Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 0006291X 10902104 https://repository.urosario.edu.co/handle/10336/23503 https://doi.org/10.1016/j.bbrc.2008.10.129 eng info:eu-repo/semantics/openAccess application/pdf instname:Universidad del Rosario
institution EdocUR - Universidad del Rosario
collection DSpace
language Inglés (English)
topic Amino acid
Antigen
Membrane protein
Protozoal protein
Article
Bioinformatics
Cellular distribution
Data base
Exon
Gametocyte
Immunohistochemistry
Life cycle
Nonhuman
Plasmodium vivax
Priority journal
Protein analysis
Protein determination
Protein domain
Protein expression
Amino acid sequence
Animals
Cell membrane
Erythrocytes
Exons
Membrane proteins
Plasmodium vivax
Protozoan proteins
Rabbits
Plasmodium falciparum
Plasmodium vivax
Bioinformatics
Malaria
Plasmodium vivax
Pv41
Six-cys domain
Vaccine candidate
molecular
genetic
tertiary
protozoan
Antigens
Cloning
Protein structure
Transcription
spellingShingle Amino acid
Antigen
Membrane protein
Protozoal protein
Article
Bioinformatics
Cellular distribution
Data base
Exon
Gametocyte
Immunohistochemistry
Life cycle
Nonhuman
Plasmodium vivax
Priority journal
Protein analysis
Protein determination
Protein domain
Protein expression
Amino acid sequence
Animals
Cell membrane
Erythrocytes
Exons
Membrane proteins
Plasmodium vivax
Protozoan proteins
Rabbits
Plasmodium falciparum
Plasmodium vivax
Bioinformatics
Malaria
Plasmodium vivax
Pv41
Six-cys domain
Vaccine candidate
molecular
genetic
tertiary
protozoan
Antigens
Cloning
Protein structure
Transcription
Angel, Diana I.
Mongui, Alvaro
Vanegas, Magnolia
Patarroyo, Manuel A.
Ardila-Montealegre, Javier
The Plasmodium vivax Pv41 surface protein: Identification and characterization
description Recently, Plasmodium vivax has been related to nearly 81% of malaria cases reported in Central America and the Mediterranean. Due to the difficulty of culturing this parasite species in vitro, most studies on P. vivax have focused on the identification of new antigens by homology comparison with P. falciparum vaccine candidate proteins. In this study, we have identified and characterized a Pf41 homologue in P. vivax, hence named Pv41, by following such approach and using web-available bioinformatics databases, molecular techniques and immunochemistry assays. Pv41 protein is a 384-amino-acid-long antigen encoded by a single exon that exhibits two s48/45 domains characteristic of gametocyte surface proteins. We have also demonstrated Pv41 transcription and expression during late intra-erythrocytic parasite stages and defined its subcellular localization on the parasite surface. © 2008 Elsevier Inc. All rights reserved.
format Artículo (Article)
author Angel, Diana I.
Mongui, Alvaro
Vanegas, Magnolia
Patarroyo, Manuel A.
Ardila-Montealegre, Javier
author_facet Angel, Diana I.
Mongui, Alvaro
Vanegas, Magnolia
Patarroyo, Manuel A.
Ardila-Montealegre, Javier
author_sort Angel, Diana I.
title The Plasmodium vivax Pv41 surface protein: Identification and characterization
title_short The Plasmodium vivax Pv41 surface protein: Identification and characterization
title_full The Plasmodium vivax Pv41 surface protein: Identification and characterization
title_fullStr The Plasmodium vivax Pv41 surface protein: Identification and characterization
title_full_unstemmed The Plasmodium vivax Pv41 surface protein: Identification and characterization
title_sort plasmodium vivax pv41 surface protein: identification and characterization
publishDate 2008
url https://repository.urosario.edu.co/handle/10336/23503
https://doi.org/10.1016/j.bbrc.2008.10.129
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score 12,131701