The Plasmodium vivax Pv41 surface protein: Identification and characterization
Recently, Plasmodium vivax has been related to nearly 81% of malaria cases reported in Central America and the Mediterranean. Due to the difficulty of culturing this parasite species in vitro, most studies on P. vivax have focused on the identification of new antigens by homology comparison with P....
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ir-10336-235032022-05-02T12:37:14Z The Plasmodium vivax Pv41 surface protein: Identification and characterization Angel, Diana I. Mongui, Alvaro Vanegas, Magnolia Patarroyo, Manuel A. Ardila-Montealegre, Javier Amino acid Antigen Membrane protein Protozoal protein Article Bioinformatics Cellular distribution Data base Exon Gametocyte Immunohistochemistry Life cycle Nonhuman Plasmodium vivax Priority journal Protein analysis Protein determination Protein domain Protein expression Amino acid sequence Animals Cell membrane Erythrocytes Exons Membrane proteins Plasmodium vivax Protozoan proteins Rabbits Plasmodium falciparum Plasmodium vivax Bioinformatics Malaria Plasmodium vivax Pv41 Six-cys domain Vaccine candidate molecular genetic tertiary protozoan Antigens Cloning Protein structure Transcription Recently, Plasmodium vivax has been related to nearly 81% of malaria cases reported in Central America and the Mediterranean. Due to the difficulty of culturing this parasite species in vitro, most studies on P. vivax have focused on the identification of new antigens by homology comparison with P. falciparum vaccine candidate proteins. In this study, we have identified and characterized a Pf41 homologue in P. vivax, hence named Pv41, by following such approach and using web-available bioinformatics databases, molecular techniques and immunochemistry assays. Pv41 protein is a 384-amino-acid-long antigen encoded by a single exon that exhibits two s48/45 domains characteristic of gametocyte surface proteins. We have also demonstrated Pv41 transcription and expression during late intra-erythrocytic parasite stages and defined its subcellular localization on the parasite surface. © 2008 Elsevier Inc. All rights reserved. 2008 2020-05-26T00:02:34Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 0006291X 10902104 https://repository.urosario.edu.co/handle/10336/23503 https://doi.org/10.1016/j.bbrc.2008.10.129 eng info:eu-repo/semantics/openAccess application/pdf instname:Universidad del Rosario |
institution |
EdocUR - Universidad del Rosario |
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DSpace |
language |
Inglés (English) |
topic |
Amino acid Antigen Membrane protein Protozoal protein Article Bioinformatics Cellular distribution Data base Exon Gametocyte Immunohistochemistry Life cycle Nonhuman Plasmodium vivax Priority journal Protein analysis Protein determination Protein domain Protein expression Amino acid sequence Animals Cell membrane Erythrocytes Exons Membrane proteins Plasmodium vivax Protozoan proteins Rabbits Plasmodium falciparum Plasmodium vivax Bioinformatics Malaria Plasmodium vivax Pv41 Six-cys domain Vaccine candidate molecular genetic tertiary protozoan Antigens Cloning Protein structure Transcription |
spellingShingle |
Amino acid Antigen Membrane protein Protozoal protein Article Bioinformatics Cellular distribution Data base Exon Gametocyte Immunohistochemistry Life cycle Nonhuman Plasmodium vivax Priority journal Protein analysis Protein determination Protein domain Protein expression Amino acid sequence Animals Cell membrane Erythrocytes Exons Membrane proteins Plasmodium vivax Protozoan proteins Rabbits Plasmodium falciparum Plasmodium vivax Bioinformatics Malaria Plasmodium vivax Pv41 Six-cys domain Vaccine candidate molecular genetic tertiary protozoan Antigens Cloning Protein structure Transcription Angel, Diana I. Mongui, Alvaro Vanegas, Magnolia Patarroyo, Manuel A. Ardila-Montealegre, Javier The Plasmodium vivax Pv41 surface protein: Identification and characterization |
description |
Recently, Plasmodium vivax has been related to nearly 81% of malaria cases reported in Central America and the Mediterranean. Due to the difficulty of culturing this parasite species in vitro, most studies on P. vivax have focused on the identification of new antigens by homology comparison with P. falciparum vaccine candidate proteins. In this study, we have identified and characterized a Pf41 homologue in P. vivax, hence named Pv41, by following such approach and using web-available bioinformatics databases, molecular techniques and immunochemistry assays. Pv41 protein is a 384-amino-acid-long antigen encoded by a single exon that exhibits two s48/45 domains characteristic of gametocyte surface proteins. We have also demonstrated Pv41 transcription and expression during late intra-erythrocytic parasite stages and defined its subcellular localization on the parasite surface. © 2008 Elsevier Inc. All rights reserved. |
format |
Artículo (Article) |
author |
Angel, Diana I. Mongui, Alvaro Vanegas, Magnolia Patarroyo, Manuel A. Ardila-Montealegre, Javier |
author_facet |
Angel, Diana I. Mongui, Alvaro Vanegas, Magnolia Patarroyo, Manuel A. Ardila-Montealegre, Javier |
author_sort |
Angel, Diana I. |
title |
The Plasmodium vivax Pv41 surface protein: Identification and characterization |
title_short |
The Plasmodium vivax Pv41 surface protein: Identification and characterization |
title_full |
The Plasmodium vivax Pv41 surface protein: Identification and characterization |
title_fullStr |
The Plasmodium vivax Pv41 surface protein: Identification and characterization |
title_full_unstemmed |
The Plasmodium vivax Pv41 surface protein: Identification and characterization |
title_sort |
plasmodium vivax pv41 surface protein: identification and characterization |
publishDate |
2008 |
url |
https://repository.urosario.edu.co/handle/10336/23503 https://doi.org/10.1016/j.bbrc.2008.10.129 |
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1740172884578402304 |
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12,131701 |