Vista Equipo: Immunological profile of a Plasmodium vivax AMA-1 N-terminus peptide-carbon nanotube conjugate in an infected Plasmodium berghei mouse model

Immunological profile of a Plasmodium vivax AMA-1 N-terminus peptide-carbon nanotube conjugate in an infected Plasmodium berghei mouse model

We have covalently conjugated an N-terminus Plasmodium vivax apical membrane antigen-1 (AMA-1) peptide to functionalized carbon nanotubes (f-CNT). Immunological characterization of this molecular conjugate revealed that the immunogen-AMA-1 peptide was appropriately presented after being conjugated t...

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Autores Principales:	Yandar, Nubia, Pastorin, Giorgia, Prato, Maurizio, Bianco, Alberto, Patarroyo, Manuel Elkin, Lozano, José Manuel
Formato:	Artículo (Article)
Lenguaje:	Inglés (English)
Publicado:	2008
Materias:	Apical membrane antigen 1 Carbon nanotube Drug carrier Polyclonal antibody Amino terminal sequence Animal experiment Animal model Article Bagg albino mouse Conjugation Controlled study Drug delivery system Drug structure Female Immune system Immunogenicity Malaria Mouse Nonhuman Parasitemia Plasmodium berghei Plasmodium vivax Priority journal Alleles Amino acid sequence Animals Cytokines Drug delivery systems Enzyme-linked immunosorbent assay Fluorescent antibody technique Hla-dr antigens Malaria vaccines Membrane proteins Mice Molecular sequence data Nanotubes Protozoan proteins Apical membrane antigen 1 (ama-1) Functionalized carbon nanotubes (f-cnt) Synthetic vaccine delivery system electron inbred balb c mhc class ii protozoan subunit western Antigens Blotting Genes Microscopy Vaccines
Acceso en línea:	https://repository.urosario.edu.co/handle/10336/23447 https://doi.org/10.1016/j.vaccine.2008.08.014

id	ir-10336-23447
recordtype	dspace
spelling	ir-10336-234472022-05-02T12:37:21Z Immunological profile of a Plasmodium vivax AMA-1 N-terminus peptide-carbon nanotube conjugate in an infected Plasmodium berghei mouse model Yandar, Nubia Pastorin, Giorgia Prato, Maurizio Bianco, Alberto Patarroyo, Manuel Elkin Lozano, José Manuel Apical membrane antigen 1 Carbon nanotube Drug carrier Polyclonal antibody Amino terminal sequence Animal experiment Animal model Article Bagg albino mouse Conjugation Controlled study Drug delivery system Drug structure Female Immune system Immunogenicity Malaria Mouse Nonhuman Parasitemia Plasmodium berghei Plasmodium vivax Priority journal Alleles Amino acid sequence Animals Cytokines Drug delivery systems Enzyme-linked immunosorbent assay Female Fluorescent antibody technique Hla-dr antigens Malaria Malaria vaccines Membrane proteins Mice Molecular sequence data Nanotubes Plasmodium berghei Plasmodium vivax Protozoan proteins Apical membrane antigen 1 (ama-1) Functionalized carbon nanotubes (f-cnt) Synthetic vaccine delivery system electron inbred balb c mhc class ii protozoan subunit western Antigens Blotting Genes Mice Microscopy Vaccines We have covalently conjugated an N-terminus Plasmodium vivax apical membrane antigen-1 (AMA-1) peptide to functionalized carbon nanotubes (f-CNT). Immunological characterization of this molecular conjugate revealed that the immunogen-AMA-1 peptide was appropriately presented after being conjugated to CNTs as well as being recognized by BALB/c polyclonal antibodies. Subsequent experiments lead us to assess the AMA-1 peptide alone, as well as the CNT-peptide conjugate regarding rodent malarial infection. Remarkably, the peptide effectively controlled and delayed Plasmodium berghei-challenged animals' parasitaemia. The peptide-CNT conjugate displayed similar immunological properties to the peptide alone by protecting or delaying malarial infection. The peptide presentation by f-CNT to the immune system thus constitutes a promising approach for synthetic malarial vaccine formulation since the immunogen peptide conformation is well preserved. © 2008 Elsevier Ltd. All rights reserved. 2008 2020-05-26T00:02:05Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 0264410X 13588745 https://repository.urosario.edu.co/handle/10336/23447 https://doi.org/10.1016/j.vaccine.2008.08.014 eng info:eu-repo/semantics/openAccess application/pdf instname:Universidad del Rosario
institution	EdocUR - Universidad del Rosario
collection	DSpace
language	Inglés (English)
topic	Apical membrane antigen 1 Carbon nanotube Drug carrier Polyclonal antibody Amino terminal sequence Animal experiment Animal model Article Bagg albino mouse Conjugation Controlled study Drug delivery system Drug structure Female Immune system Immunogenicity Malaria Mouse Nonhuman Parasitemia Plasmodium berghei Plasmodium vivax Priority journal Alleles Amino acid sequence Animals Cytokines Drug delivery systems Enzyme-linked immunosorbent assay Female Fluorescent antibody technique Hla-dr antigens Malaria Malaria vaccines Membrane proteins Mice Molecular sequence data Nanotubes Plasmodium berghei Plasmodium vivax Protozoan proteins Apical membrane antigen 1 (ama-1) Functionalized carbon nanotubes (f-cnt) Synthetic vaccine delivery system electron inbred balb c mhc class ii protozoan subunit western Antigens Blotting Genes Mice Microscopy Vaccines
spellingShingle	Apical membrane antigen 1 Carbon nanotube Drug carrier Polyclonal antibody Amino terminal sequence Animal experiment Animal model Article Bagg albino mouse Conjugation Controlled study Drug delivery system Drug structure Female Immune system Immunogenicity Malaria Mouse Nonhuman Parasitemia Plasmodium berghei Plasmodium vivax Priority journal Alleles Amino acid sequence Animals Cytokines Drug delivery systems Enzyme-linked immunosorbent assay Female Fluorescent antibody technique Hla-dr antigens Malaria Malaria vaccines Membrane proteins Mice Molecular sequence data Nanotubes Plasmodium berghei Plasmodium vivax Protozoan proteins Apical membrane antigen 1 (ama-1) Functionalized carbon nanotubes (f-cnt) Synthetic vaccine delivery system electron inbred balb c mhc class ii protozoan subunit western Antigens Blotting Genes Mice Microscopy Vaccines Yandar, Nubia Pastorin, Giorgia Prato, Maurizio Bianco, Alberto Patarroyo, Manuel Elkin Lozano, José Manuel Immunological profile of a Plasmodium vivax AMA-1 N-terminus peptide-carbon nanotube conjugate in an infected Plasmodium berghei mouse model
description	We have covalently conjugated an N-terminus Plasmodium vivax apical membrane antigen-1 (AMA-1) peptide to functionalized carbon nanotubes (f-CNT). Immunological characterization of this molecular conjugate revealed that the immunogen-AMA-1 peptide was appropriately presented after being conjugated to CNTs as well as being recognized by BALB/c polyclonal antibodies. Subsequent experiments lead us to assess the AMA-1 peptide alone, as well as the CNT-peptide conjugate regarding rodent malarial infection. Remarkably, the peptide effectively controlled and delayed Plasmodium berghei-challenged animals' parasitaemia. The peptide-CNT conjugate displayed similar immunological properties to the peptide alone by protecting or delaying malarial infection. The peptide presentation by f-CNT to the immune system thus constitutes a promising approach for synthetic malarial vaccine formulation since the immunogen peptide conformation is well preserved. © 2008 Elsevier Ltd. All rights reserved.
format	Artículo (Article)
author	Yandar, Nubia Pastorin, Giorgia Prato, Maurizio Bianco, Alberto Patarroyo, Manuel Elkin Lozano, José Manuel
author_facet	Yandar, Nubia Pastorin, Giorgia Prato, Maurizio Bianco, Alberto Patarroyo, Manuel Elkin Lozano, José Manuel
author_sort	Yandar, Nubia
title	Immunological profile of a Plasmodium vivax AMA-1 N-terminus peptide-carbon nanotube conjugate in an infected Plasmodium berghei mouse model
title_short	Immunological profile of a Plasmodium vivax AMA-1 N-terminus peptide-carbon nanotube conjugate in an infected Plasmodium berghei mouse model
title_full	Immunological profile of a Plasmodium vivax AMA-1 N-terminus peptide-carbon nanotube conjugate in an infected Plasmodium berghei mouse model
title_fullStr	Immunological profile of a Plasmodium vivax AMA-1 N-terminus peptide-carbon nanotube conjugate in an infected Plasmodium berghei mouse model
title_full_unstemmed	Immunological profile of a Plasmodium vivax AMA-1 N-terminus peptide-carbon nanotube conjugate in an infected Plasmodium berghei mouse model
title_sort	immunological profile of a plasmodium vivax ama-1 n-terminus peptide-carbon nanotube conjugate in an infected plasmodium berghei mouse model
publishDate	2008
url	https://repository.urosario.edu.co/handle/10336/23447 https://doi.org/10.1016/j.vaccine.2008.08.014
_version_	1740173068421038080
score	12,131701

Immunological profile of a Plasmodium vivax AMA-1 N-terminus peptide-carbon nanotube conjugate in an infected Plasmodium berghei mouse model

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