Biological characterisation of yellow fever 17D strain plaque variants

"The Colombian 17D vaccine contains at least four phenotypes denominated small (0.3 - 1.2 mm), medium (1.3 - 2.1 mm) large (2.2 - 3.0 mm) and extra-large ( and gt;3.1 mm). These phenotypes composition and percentage distribution vary between different production lots and between ampoules from a...

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Autores Principales: Rojas M.O., Camacho M., Grosso M.V.
Formato: Artículo (Article)
Lenguaje:Inglés (English)
Publicado: 2009
Materias:
Acceso en línea:https://repository.urosario.edu.co/handle/10336/23061
id ir-10336-23061
recordtype dspace
spelling ir-10336-230612021-01-21T08:25:37Z Biological characterisation of yellow fever 17D strain plaque variants Caracterización biológica de variantes de placa de la cepa vacunal 17D contra la fiebre amarilla Rojas M.O. Camacho M. Grosso M.V. 17D vaccine Adverse vaccine events Flavivirus Yellow fever virus "The Colombian 17D vaccine contains at least four phenotypes denominated small (0.3 - 1.2 mm), medium (1.3 - 2.1 mm) large (2.2 - 3.0 mm) and extra-large ( and gt;3.1 mm). These phenotypes composition and percentage distribution vary between different production lots and between ampoules from a particular lot. Each variant was cloned by diluting the vaccine and its virulent effect was analysed in young mice. The small plaque phenotype was slightly under represented in the lots analysed and showed similar virulence to the neurotropic French wild strain (LD50 and gt;10-6), whilst the medium- sized phe-notype predominated and was the most attenuated (LD50:10-4). The large and extra-large phenotypes showed intermediate virulence (LD50:10-5) regarding the others. Sequence analysis of variants within the region between the 3'NS5 end and the beginning of 3'NCR showed the closeness between those variants having some degree of virulence and between the attenuated variant and the Colombian vaccine. The 17D vaccine's heterogeneity constitutes evidence of RNA virus quasi-specie structure and shows how adverse yellow fever vaccine reaction can be associated with applying vaccines produced from attenuated viral strains." 2009 2020-05-25T23:59:32Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 1202804 https://repository.urosario.edu.co/handle/10336/23061 eng info:eu-repo/semantics/openAccess application/pdf instname:Universidad del Rosario reponame:Repositorio Institucional EdocUR
institution EdocUR - Universidad del Rosario
collection DSpace
language Inglés (English)
topic 17D vaccine
Adverse vaccine events
Flavivirus
Yellow fever virus
spellingShingle 17D vaccine
Adverse vaccine events
Flavivirus
Yellow fever virus
Rojas M.O.
Camacho M.
Grosso M.V.
Biological characterisation of yellow fever 17D strain plaque variants
description "The Colombian 17D vaccine contains at least four phenotypes denominated small (0.3 - 1.2 mm), medium (1.3 - 2.1 mm) large (2.2 - 3.0 mm) and extra-large ( and gt;3.1 mm). These phenotypes composition and percentage distribution vary between different production lots and between ampoules from a particular lot. Each variant was cloned by diluting the vaccine and its virulent effect was analysed in young mice. The small plaque phenotype was slightly under represented in the lots analysed and showed similar virulence to the neurotropic French wild strain (LD50 and gt;10-6), whilst the medium- sized phe-notype predominated and was the most attenuated (LD50:10-4). The large and extra-large phenotypes showed intermediate virulence (LD50:10-5) regarding the others. Sequence analysis of variants within the region between the 3'NS5 end and the beginning of 3'NCR showed the closeness between those variants having some degree of virulence and between the attenuated variant and the Colombian vaccine. The 17D vaccine's heterogeneity constitutes evidence of RNA virus quasi-specie structure and shows how adverse yellow fever vaccine reaction can be associated with applying vaccines produced from attenuated viral strains."
format Artículo (Article)
author Rojas M.O.
Camacho M.
Grosso M.V.
author_facet Rojas M.O.
Camacho M.
Grosso M.V.
author_sort Rojas M.O.
title Biological characterisation of yellow fever 17D strain plaque variants
title_short Biological characterisation of yellow fever 17D strain plaque variants
title_full Biological characterisation of yellow fever 17D strain plaque variants
title_fullStr Biological characterisation of yellow fever 17D strain plaque variants
title_full_unstemmed Biological characterisation of yellow fever 17D strain plaque variants
title_sort biological characterisation of yellow fever 17d strain plaque variants
publishDate 2009
url https://repository.urosario.edu.co/handle/10336/23061
_version_ 1690577211818508288
score 11,828437