Interaction of leishmania (L.) chagasi with the lulo cell line in different environmental conditions

There are unknown or poorly understood events in the Leishmania-host cell interactions that merit studies in new in vitro models. The aim of the present work was to evaluate Leishmania chagasi interaction with a cell line (Lulo) previously established and derived from Lutzomyia longipalpis embryonic...

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Autores Principales: Acero V., Galeano E., Ayala M., Castellanos J., Bello F.
Formato: Artículo (Article)
Lenguaje:Español (Spanish)
Publicado: 2006
Materias:
Acceso en línea:https://repository.urosario.edu.co/handle/10336/23007
id ir-10336-23007
recordtype dspace
spelling ir-10336-230072022-05-02T12:37:14Z Interaction of leishmania (L.) chagasi with the lulo cell line in different environmental conditions Interacción de leishmania (L.) chagasi con la línea celular lulo en diferentes condiciones ambientales Acero V. Galeano E. Ayala M. Castellanos J. Bello F. Leishmania donovani chagasi Lutzomyia longipalpis Solanum quitoense Cell morphometry Percentage of infection Promastigote Temperature and co 2 Ultrastructure characteristics There are unknown or poorly understood events in the Leishmania-host cell interactions that merit studies in new in vitro models. The aim of the present work was to evaluate Leishmania chagasi interaction with a cell line (Lulo) previously established and derived from Lutzomyia longipalpis embryonic tissues, bearing different temperatures and CO 2 concentrations in mind and morphometric changes of the cells. The J774 cell line was used as positive control of the infection. The L. chagasi MH/CO/84/CL-044B strain was used for infection assays. The parasites were added to adherent cells, in Lulo as well as J774, in a 5:1 ratio. Incubation was at 28°C and 37°C, in the absence and presence of CO 2. A morphometric study of the cells was carried out before and after infection. Both parasite and cell ultrastructure characteristics were analyzed by electron microscope. The greatest percentage of infection in the Lulo cells at 28°C (26,8%) was registered on day six post infection, while at 37°C, in the absence of CO 2, the maximum value (30,4%) was obtained on day nine. The morphometric analyses showed a significant increase in the size of Lulo cells infected at 28°C and J774 cells at 37°C with and without CO 2, respectively. 2006 2020-05-25T23:59:13Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 1200488 https://repository.urosario.edu.co/handle/10336/23007 spa info:eu-repo/semantics/openAccess application/pdf instname:Universidad del Rosario
institution EdocUR - Universidad del Rosario
collection DSpace
language Español (Spanish)
topic Leishmania donovani chagasi
Lutzomyia longipalpis
Solanum quitoense
Cell morphometry
Percentage of infection
Promastigote
Temperature and co 2
Ultrastructure characteristics
spellingShingle Leishmania donovani chagasi
Lutzomyia longipalpis
Solanum quitoense
Cell morphometry
Percentage of infection
Promastigote
Temperature and co 2
Ultrastructure characteristics
Acero V.
Galeano E.
Ayala M.
Castellanos J.
Bello F.
Interaction of leishmania (L.) chagasi with the lulo cell line in different environmental conditions
description There are unknown or poorly understood events in the Leishmania-host cell interactions that merit studies in new in vitro models. The aim of the present work was to evaluate Leishmania chagasi interaction with a cell line (Lulo) previously established and derived from Lutzomyia longipalpis embryonic tissues, bearing different temperatures and CO 2 concentrations in mind and morphometric changes of the cells. The J774 cell line was used as positive control of the infection. The L. chagasi MH/CO/84/CL-044B strain was used for infection assays. The parasites were added to adherent cells, in Lulo as well as J774, in a 5:1 ratio. Incubation was at 28°C and 37°C, in the absence and presence of CO 2. A morphometric study of the cells was carried out before and after infection. Both parasite and cell ultrastructure characteristics were analyzed by electron microscope. The greatest percentage of infection in the Lulo cells at 28°C (26,8%) was registered on day six post infection, while at 37°C, in the absence of CO 2, the maximum value (30,4%) was obtained on day nine. The morphometric analyses showed a significant increase in the size of Lulo cells infected at 28°C and J774 cells at 37°C with and without CO 2, respectively.
format Artículo (Article)
author Acero V.
Galeano E.
Ayala M.
Castellanos J.
Bello F.
author_facet Acero V.
Galeano E.
Ayala M.
Castellanos J.
Bello F.
author_sort Acero V.
title Interaction of leishmania (L.) chagasi with the lulo cell line in different environmental conditions
title_short Interaction of leishmania (L.) chagasi with the lulo cell line in different environmental conditions
title_full Interaction of leishmania (L.) chagasi with the lulo cell line in different environmental conditions
title_fullStr Interaction of leishmania (L.) chagasi with the lulo cell line in different environmental conditions
title_full_unstemmed Interaction of leishmania (L.) chagasi with the lulo cell line in different environmental conditions
title_sort interaction of leishmania (l.) chagasi with the lulo cell line in different environmental conditions
publishDate 2006
url https://repository.urosario.edu.co/handle/10336/23007
_version_ 1740172474295779328
score 12,131701