Evaluation of the multispecies coalescent method to explore intra-Trypanosoma cruzi i relationships and genetic diversity

Chagas Disease is a zoonosis caused by the parasite Trypanosoma cruzi. Several high-resolution markers have subdivided T. cruzi taxon into at least seven lineages or Discrete Typing Units (DTUs) (TcI-TcVI and TcBat). Trypanosoma cruzi I is the most diverse and geographically widespread DTU. Recently...

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Autores Principales: Gómez-Hernández C., Pérez S.D., Rezende-Oliveira K., Barbosa C.G., Lages-Silva E., Ramírez L.E., Ramírez, Juan David
Formato: Artículo (Article)
Lenguaje:Inglés (English)
Publicado: Cambridge University Press 2019
Materias:
Acceso en línea:https://repository.urosario.edu.co/handle/10336/22761
https://doi.org/10.1017/S0031182019000428
id ir-10336-22761
recordtype dspace
spelling ir-10336-227612022-05-02T12:37:16Z Evaluation of the multispecies coalescent method to explore intra-Trypanosoma cruzi i relationships and genetic diversity Gómez-Hernández C. Pérez S.D. Rezende-Oliveira K. Barbosa C.G. Lages-Silva E. Ramírez L.E. Ramírez, Juan David Genomic DNA Molecular marker Article Chagas disease Cladistics Gene structure Genetic variability Housekeeping gene Nonhuman Parasite cultivation Parasite isolation Priority journal Trypanosoma cruzi Chagas disease Discrete typing unit Genotype Housekeeping genes Lineage Multispecies coalescent Chagas Disease is a zoonosis caused by the parasite Trypanosoma cruzi. Several high-resolution markers have subdivided T. cruzi taxon into at least seven lineages or Discrete Typing Units (DTUs) (TcI-TcVI and TcBat). Trypanosoma cruzi I is the most diverse and geographically widespread DTU. Recently a TcI genotype related to domestic cycles was proposed and named as TcIDOM. Herein, we combined traditional markers and housekeeping genes and applied a Multispecies Coalescent method to explore intra-TcI relationships, lineage boundaries and genetic diversity in a random set of isolates and DNA sequences retrieved from Genbank from different countries in the Americas. We found further evidence supporting TcIDOM as an independent and emerging genotype of TcI at least in Colombia and Venezuela. We also found evidence of high phylogenetic incongruence between parasite's gene trees (including introgression) and embedded species trees, and a lack of genetic structure among geography and hosts, illustrating the complex dynamics and epidemiology of TcI across the Americas. These findings provide novel insights into T. cruzi systematics and epidemiology and support the need to assess parasite diversity and lineage boundaries through hypothesis testing using different approaches to those traditionally employed, including the Bayesian Multispecies coalescent method. Copyright © Cambridge University Press 2019. 2019 2020-05-25T23:57:53Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 14698161 00311820 https://repository.urosario.edu.co/handle/10336/22761 https://doi.org/10.1017/S0031182019000428 eng info:eu-repo/semantics/openAccess application/pdf Cambridge University Press instname:Universidad del Rosario
institution EdocUR - Universidad del Rosario
collection DSpace
language Inglés (English)
topic Genomic DNA
Molecular marker
Article
Chagas disease
Cladistics
Gene structure
Genetic variability
Housekeeping gene
Nonhuman
Parasite cultivation
Parasite isolation
Priority journal
Trypanosoma cruzi
Chagas disease
Discrete typing unit
Genotype
Housekeeping genes
Lineage
Multispecies coalescent
spellingShingle Genomic DNA
Molecular marker
Article
Chagas disease
Cladistics
Gene structure
Genetic variability
Housekeeping gene
Nonhuman
Parasite cultivation
Parasite isolation
Priority journal
Trypanosoma cruzi
Chagas disease
Discrete typing unit
Genotype
Housekeeping genes
Lineage
Multispecies coalescent
Gómez-Hernández C.
Pérez S.D.
Rezende-Oliveira K.
Barbosa C.G.
Lages-Silva E.
Ramírez L.E.
Ramírez, Juan David
Evaluation of the multispecies coalescent method to explore intra-Trypanosoma cruzi i relationships and genetic diversity
description Chagas Disease is a zoonosis caused by the parasite Trypanosoma cruzi. Several high-resolution markers have subdivided T. cruzi taxon into at least seven lineages or Discrete Typing Units (DTUs) (TcI-TcVI and TcBat). Trypanosoma cruzi I is the most diverse and geographically widespread DTU. Recently a TcI genotype related to domestic cycles was proposed and named as TcIDOM. Herein, we combined traditional markers and housekeeping genes and applied a Multispecies Coalescent method to explore intra-TcI relationships, lineage boundaries and genetic diversity in a random set of isolates and DNA sequences retrieved from Genbank from different countries in the Americas. We found further evidence supporting TcIDOM as an independent and emerging genotype of TcI at least in Colombia and Venezuela. We also found evidence of high phylogenetic incongruence between parasite's gene trees (including introgression) and embedded species trees, and a lack of genetic structure among geography and hosts, illustrating the complex dynamics and epidemiology of TcI across the Americas. These findings provide novel insights into T. cruzi systematics and epidemiology and support the need to assess parasite diversity and lineage boundaries through hypothesis testing using different approaches to those traditionally employed, including the Bayesian Multispecies coalescent method. Copyright © Cambridge University Press 2019.
format Artículo (Article)
author Gómez-Hernández C.
Pérez S.D.
Rezende-Oliveira K.
Barbosa C.G.
Lages-Silva E.
Ramírez L.E.
Ramírez, Juan David
author_facet Gómez-Hernández C.
Pérez S.D.
Rezende-Oliveira K.
Barbosa C.G.
Lages-Silva E.
Ramírez L.E.
Ramírez, Juan David
author_sort Gómez-Hernández C.
title Evaluation of the multispecies coalescent method to explore intra-Trypanosoma cruzi i relationships and genetic diversity
title_short Evaluation of the multispecies coalescent method to explore intra-Trypanosoma cruzi i relationships and genetic diversity
title_full Evaluation of the multispecies coalescent method to explore intra-Trypanosoma cruzi i relationships and genetic diversity
title_fullStr Evaluation of the multispecies coalescent method to explore intra-Trypanosoma cruzi i relationships and genetic diversity
title_full_unstemmed Evaluation of the multispecies coalescent method to explore intra-Trypanosoma cruzi i relationships and genetic diversity
title_sort evaluation of the multispecies coalescent method to explore intra-trypanosoma cruzi i relationships and genetic diversity
publisher Cambridge University Press
publishDate 2019
url https://repository.urosario.edu.co/handle/10336/22761
https://doi.org/10.1017/S0031182019000428
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