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A novel loop-mediated isothermal amplification-based test for detecting Neospora caninum DNA

Background: Neospora caninum is a cyst-forming, coccidian parasite which is known to cause neurological disorders in dogs and abortion and neonatal mortality in cows and other livestock. This study reports the development of a loop-mediated isothermal amplification (LAMP) assay based on the Neospora...

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Detalles Bibliográficos
Autores Principales: Ramos, Andrea Estefanía, Muñoz, Marina, Cortés-Vecino, Jesús Alfredo, Barato, Paola, Patarroyo, Manuel A.
Formato: Artículo (Article)
Lenguaje:Inglés (English)
Publicado: BioMed Central Ltd. 2017
Materias:
Dna
Parasite antigen
Primer dna
Protozoal dna
Article
Controlled study
Cryptosporidium parvum
Dna determination
Dna sequence
Gene
Gene amplification
Hammondia hammondi
Limit of detection
Loop mediated isothermal amplification
Nc 5 gene
Neospora caninum
Neosporosis
Nonhuman
Polymerase chain reaction
Protozoon
Sarcocystis
Sarcocystis cruzi
Sarcocystis hominis
Sequence alignment
Toxoplasma gondii
Animal
Bovine
Cattle disease
Coccidiosis
Dog
Dog disease
Feces
Female
Genetics
Isolation and purification
Neospora
Nucleic acid amplification
Parasitology
Pregnancy
Procedures
Sensitivity and specificity
Temperature
Veterinary
Animals
Cattle
Cattle diseases
Dna primers
Dog diseases
Dogs
Nucleic acid amplification techniques
Loop-mediated isothermal amplification
Nc-5 gene
Semi-nested pcr
protozoan
Antigens
Acceso en línea:https://repository.urosario.edu.co/handle/10336/22749
https://doi.org/10.1186/s13071-017-2549-y

Internet

https://repository.urosario.edu.co/handle/10336/22749
https://doi.org/10.1186/s13071-017-2549-y

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