Synthetic evaluation of standard and microwave-assisted solid phase peptide synthesis of a long chimeric peptide derived from four Plasmodium falciparum proteins
An 82-residue-long chimeric peptide was synthesised by solid phase peptide synthesis (SPPS), following the Fmoc protocol. Microwave (MW) radiation-assisted synthesis was compared to standard synthesis using low loading (0.20 mmol/g) of polyethylene glycol (PEG) resin. Similar synthetic difficulties...
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| Formato: | Artículo (Article) |
| Lenguaje: | Inglés (English) |
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MDPI AG
2018
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| Acceso en línea: | https://repository.urosario.edu.co/handle/10336/22628 https://doi.org/10.3390/molecules23112877 |
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ir-10336-226282022-05-02T12:37:20Z Synthetic evaluation of standard and microwave-assisted solid phase peptide synthesis of a long chimeric peptide derived from four Plasmodium falciparum proteins Varela, Yahson F. Murcia, Magnolia Vanegas Patarroyo, Manuel Elkin Peptide Protozoal protein Chemistry Drug effect Metabolism Microwave radiation Molecular weight Plasmodium falciparum Solid phase synthesis Spectroscopy Synthesis Microwaves Molecular weight Peptides Plasmodium falciparum Protozoan proteins Solid-phase synthesis techniques Spectrum analysis Chimeric peptide Coupling Fmoc protocol Microwave radiation Solid phase peptide synthesis An 82-residue-long chimeric peptide was synthesised by solid phase peptide synthesis (SPPS), following the Fmoc protocol. Microwave (MW) radiation-assisted synthesis was compared to standard synthesis using low loading (0.20 mmol/g) of polyethylene glycol (PEG) resin. Similar synthetic difficulties were found when the chimeric peptide was obtained via these two reaction conditions, indicating that such difficulties were inherent to the sequence and could not be resolved using MW; by contrast, the number of coupling cycles and total reaction time became reduced whilst crude yield and percentage recovery after purification were higher for MW radiation-assisted synthesis. © 2018 by the authors. 2018 2020-05-25T23:57:12Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 14203049 https://repository.urosario.edu.co/handle/10336/22628 https://doi.org/10.3390/molecules23112877 eng info:eu-repo/semantics/openAccess application/pdf MDPI AG instname:Universidad del Rosario |
| institution |
EdocUR - Universidad del Rosario |
| collection |
DSpace |
| language |
Inglés (English) |
| topic |
Peptide Protozoal protein Chemistry Drug effect Metabolism Microwave radiation Molecular weight Plasmodium falciparum Solid phase synthesis Spectroscopy Synthesis Microwaves Molecular weight Peptides Plasmodium falciparum Protozoan proteins Solid-phase synthesis techniques Spectrum analysis Chimeric peptide Coupling Fmoc protocol Microwave radiation Solid phase peptide synthesis |
| spellingShingle |
Peptide Protozoal protein Chemistry Drug effect Metabolism Microwave radiation Molecular weight Plasmodium falciparum Solid phase synthesis Spectroscopy Synthesis Microwaves Molecular weight Peptides Plasmodium falciparum Protozoan proteins Solid-phase synthesis techniques Spectrum analysis Chimeric peptide Coupling Fmoc protocol Microwave radiation Solid phase peptide synthesis Varela, Yahson F. Murcia, Magnolia Vanegas Patarroyo, Manuel Elkin Synthetic evaluation of standard and microwave-assisted solid phase peptide synthesis of a long chimeric peptide derived from four Plasmodium falciparum proteins |
| description |
An 82-residue-long chimeric peptide was synthesised by solid phase peptide synthesis (SPPS), following the Fmoc protocol. Microwave (MW) radiation-assisted synthesis was compared to standard synthesis using low loading (0.20 mmol/g) of polyethylene glycol (PEG) resin. Similar synthetic difficulties were found when the chimeric peptide was obtained via these two reaction conditions, indicating that such difficulties were inherent to the sequence and could not be resolved using MW; by contrast, the number of coupling cycles and total reaction time became reduced whilst crude yield and percentage recovery after purification were higher for MW radiation-assisted synthesis. © 2018 by the authors. |
| format |
Artículo (Article) |
| author |
Varela, Yahson F. Murcia, Magnolia Vanegas Patarroyo, Manuel Elkin |
| author_facet |
Varela, Yahson F. Murcia, Magnolia Vanegas Patarroyo, Manuel Elkin |
| author_sort |
Varela, Yahson F. |
| title |
Synthetic evaluation of standard and microwave-assisted solid phase peptide synthesis of a long chimeric peptide derived from four Plasmodium falciparum proteins |
| title_short |
Synthetic evaluation of standard and microwave-assisted solid phase peptide synthesis of a long chimeric peptide derived from four Plasmodium falciparum proteins |
| title_full |
Synthetic evaluation of standard and microwave-assisted solid phase peptide synthesis of a long chimeric peptide derived from four Plasmodium falciparum proteins |
| title_fullStr |
Synthetic evaluation of standard and microwave-assisted solid phase peptide synthesis of a long chimeric peptide derived from four Plasmodium falciparum proteins |
| title_full_unstemmed |
Synthetic evaluation of standard and microwave-assisted solid phase peptide synthesis of a long chimeric peptide derived from four Plasmodium falciparum proteins |
| title_sort |
synthetic evaluation of standard and microwave-assisted solid phase peptide synthesis of a long chimeric peptide derived from four plasmodium falciparum proteins |
| publisher |
MDPI AG |
| publishDate |
2018 |
| url |
https://repository.urosario.edu.co/handle/10336/22628 https://doi.org/10.3390/molecules23112877 |
| _version_ |
1740172248259493888 |
| score |
12,131701 |