Identifying and characterising PPE7 (Rv0354c) high activity binding peptides and their role in inhibiting cell invasion
This study was aimed at characterising the PPE7 protein from the PE/PPE protein family. The presence and transcription of the rv0354c gene in the Mycobacterium tuberculosis complex was determined and the subcellular localisation of the PPE7 protein on mycobacterial membrane was confirmed by immunoel...
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Acceso en línea: | https://repository.urosario.edu.co/handle/10336/22603 https://doi.org/10.1007/s11010-017-2962-8 |
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ir-10336-226032022-05-02T12:37:14Z Identifying and characterising PPE7 (Rv0354c) high activity binding peptides and their role in inhibiting cell invasion Díaz D.P. Ocampo M. Varela Y. Curtidor H. Patarroyo M.A. Patarroyo M.E. Bacterial protein Peptide Ppe7 protein Unclassified drug Bcg vaccine A-549 cell line Amino acid sequence Article Bacterial gene Binding affinity Carboxy terminal sequence Cell invasion Cellular distribution Controlled study Host cell Human Human cell Immunoelectron microscopy In vitro study Macrophage Microscope Mycobacterium tuberculosis Mycobacterium tuberculosis complex Nonhuman Protein binding U-937 cell line Cell membrane Genetics Metabolism Mycobacterium tuberculosis Pathogenicity Pathology Ultrastructure A549 cells Bacterial proteins Cell membrane Humans Mycobacterium tuberculosis Tuberculosis vaccines U937 cells High activity binding peptide Mycobacterium tuberculosis h37rv Ppe7 protein Synthetic peptide This study was aimed at characterising the PPE7 protein from the PE/PPE protein family. The presence and transcription of the rv0354c gene in the Mycobacterium tuberculosis complex was determined and the subcellular localisation of the PPE7 protein on mycobacterial membrane was confirmed by immunoelectron microscope. Two peptides were identified as having high binding activity (HABPs) and were tested in vitro regarding the invasion of Mycobacterium tuberculosis H37Rv. HABP 39224 inhibited invasion in A549 epithelial cells and U937 macrophages by more than 50%, whilst HABP 39225 inhibited invasion by 40% in U937 cells. HABP 39224, located in the protein’s C-terminal region, has a completely conserved amino acid sequence in M. tuberculosis complex species and could be selected as a base peptide when designing a subunit-based, anti-tuberculosis vaccine. © 2017, Springer Science+Business Media New York. 2017 2020-05-25T23:57:05Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 15734919 03008177 https://repository.urosario.edu.co/handle/10336/22603 https://doi.org/10.1007/s11010-017-2962-8 eng info:eu-repo/semantics/openAccess application/pdf Springer New York LLC instname:Universidad del Rosario |
institution |
EdocUR - Universidad del Rosario |
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language |
Inglés (English) |
topic |
Bacterial protein Peptide Ppe7 protein Unclassified drug Bcg vaccine A-549 cell line Amino acid sequence Article Bacterial gene Binding affinity Carboxy terminal sequence Cell invasion Cellular distribution Controlled study Host cell Human Human cell Immunoelectron microscopy In vitro study Macrophage Microscope Mycobacterium tuberculosis Mycobacterium tuberculosis complex Nonhuman Protein binding U-937 cell line Cell membrane Genetics Metabolism Mycobacterium tuberculosis Pathogenicity Pathology Ultrastructure A549 cells Bacterial proteins Cell membrane Humans Mycobacterium tuberculosis Tuberculosis vaccines U937 cells High activity binding peptide Mycobacterium tuberculosis h37rv Ppe7 protein Synthetic peptide |
spellingShingle |
Bacterial protein Peptide Ppe7 protein Unclassified drug Bcg vaccine A-549 cell line Amino acid sequence Article Bacterial gene Binding affinity Carboxy terminal sequence Cell invasion Cellular distribution Controlled study Host cell Human Human cell Immunoelectron microscopy In vitro study Macrophage Microscope Mycobacterium tuberculosis Mycobacterium tuberculosis complex Nonhuman Protein binding U-937 cell line Cell membrane Genetics Metabolism Mycobacterium tuberculosis Pathogenicity Pathology Ultrastructure A549 cells Bacterial proteins Cell membrane Humans Mycobacterium tuberculosis Tuberculosis vaccines U937 cells High activity binding peptide Mycobacterium tuberculosis h37rv Ppe7 protein Synthetic peptide Díaz D.P. Ocampo M. Varela Y. Curtidor H. Patarroyo M.A. Patarroyo M.E. Identifying and characterising PPE7 (Rv0354c) high activity binding peptides and their role in inhibiting cell invasion |
description |
This study was aimed at characterising the PPE7 protein from the PE/PPE protein family. The presence and transcription of the rv0354c gene in the Mycobacterium tuberculosis complex was determined and the subcellular localisation of the PPE7 protein on mycobacterial membrane was confirmed by immunoelectron microscope. Two peptides were identified as having high binding activity (HABPs) and were tested in vitro regarding the invasion of Mycobacterium tuberculosis H37Rv. HABP 39224 inhibited invasion in A549 epithelial cells and U937 macrophages by more than 50%, whilst HABP 39225 inhibited invasion by 40% in U937 cells. HABP 39224, located in the protein’s C-terminal region, has a completely conserved amino acid sequence in M. tuberculosis complex species and could be selected as a base peptide when designing a subunit-based, anti-tuberculosis vaccine. © 2017, Springer Science+Business Media New York. |
format |
Artículo (Article) |
author |
Díaz D.P. Ocampo M. Varela Y. Curtidor H. Patarroyo M.A. Patarroyo M.E. |
author_facet |
Díaz D.P. Ocampo M. Varela Y. Curtidor H. Patarroyo M.A. Patarroyo M.E. |
author_sort |
Díaz D.P. |
title |
Identifying and characterising PPE7 (Rv0354c) high activity binding peptides and their role in inhibiting cell invasion |
title_short |
Identifying and characterising PPE7 (Rv0354c) high activity binding peptides and their role in inhibiting cell invasion |
title_full |
Identifying and characterising PPE7 (Rv0354c) high activity binding peptides and their role in inhibiting cell invasion |
title_fullStr |
Identifying and characterising PPE7 (Rv0354c) high activity binding peptides and their role in inhibiting cell invasion |
title_full_unstemmed |
Identifying and characterising PPE7 (Rv0354c) high activity binding peptides and their role in inhibiting cell invasion |
title_sort |
identifying and characterising ppe7 (rv0354c) high activity binding peptides and their role in inhibiting cell invasion |
publisher |
Springer New York LLC |
publishDate |
2017 |
url |
https://repository.urosario.edu.co/handle/10336/22603 https://doi.org/10.1007/s11010-017-2962-8 |
_version_ |
1740172914235277312 |
score |
12,131701 |