Identifying and characterising PPE7 (Rv0354c) high activity binding peptides and their role in inhibiting cell invasion

This study was aimed at characterising the PPE7 protein from the PE/PPE protein family. The presence and transcription of the rv0354c gene in the Mycobacterium tuberculosis complex was determined and the subcellular localisation of the PPE7 protein on mycobacterial membrane was confirmed by immunoel...

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Autores Principales: Díaz D.P., Ocampo M., Varela Y., Curtidor H., Patarroyo M.A., Patarroyo M.E.
Formato: Artículo (Article)
Lenguaje:Inglés (English)
Publicado: Springer New York LLC 2017
Materias:
Acceso en línea:https://repository.urosario.edu.co/handle/10336/22603
https://doi.org/10.1007/s11010-017-2962-8
id ir-10336-22603
recordtype dspace
spelling ir-10336-226032022-05-02T12:37:14Z Identifying and characterising PPE7 (Rv0354c) high activity binding peptides and their role in inhibiting cell invasion Díaz D.P. Ocampo M. Varela Y. Curtidor H. Patarroyo M.A. Patarroyo M.E. Bacterial protein Peptide Ppe7 protein Unclassified drug Bcg vaccine A-549 cell line Amino acid sequence Article Bacterial gene Binding affinity Carboxy terminal sequence Cell invasion Cellular distribution Controlled study Host cell Human Human cell Immunoelectron microscopy In vitro study Macrophage Microscope Mycobacterium tuberculosis Mycobacterium tuberculosis complex Nonhuman Protein binding U-937 cell line Cell membrane Genetics Metabolism Mycobacterium tuberculosis Pathogenicity Pathology Ultrastructure A549 cells Bacterial proteins Cell membrane Humans Mycobacterium tuberculosis Tuberculosis vaccines U937 cells High activity binding peptide Mycobacterium tuberculosis h37rv Ppe7 protein Synthetic peptide This study was aimed at characterising the PPE7 protein from the PE/PPE protein family. The presence and transcription of the rv0354c gene in the Mycobacterium tuberculosis complex was determined and the subcellular localisation of the PPE7 protein on mycobacterial membrane was confirmed by immunoelectron microscope. Two peptides were identified as having high binding activity (HABPs) and were tested in vitro regarding the invasion of Mycobacterium tuberculosis H37Rv. HABP 39224 inhibited invasion in A549 epithelial cells and U937 macrophages by more than 50%, whilst HABP 39225 inhibited invasion by 40% in U937 cells. HABP 39224, located in the protein’s C-terminal region, has a completely conserved amino acid sequence in M. tuberculosis complex species and could be selected as a base peptide when designing a subunit-based, anti-tuberculosis vaccine. © 2017, Springer Science+Business Media New York. 2017 2020-05-25T23:57:05Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 15734919 03008177 https://repository.urosario.edu.co/handle/10336/22603 https://doi.org/10.1007/s11010-017-2962-8 eng info:eu-repo/semantics/openAccess application/pdf Springer New York LLC instname:Universidad del Rosario
institution EdocUR - Universidad del Rosario
collection DSpace
language Inglés (English)
topic Bacterial protein
Peptide
Ppe7 protein
Unclassified drug
Bcg vaccine
A-549 cell line
Amino acid sequence
Article
Bacterial gene
Binding affinity
Carboxy terminal sequence
Cell invasion
Cellular distribution
Controlled study
Host cell
Human
Human cell
Immunoelectron microscopy
In vitro study
Macrophage
Microscope
Mycobacterium tuberculosis
Mycobacterium tuberculosis complex
Nonhuman
Protein binding
U-937 cell line
Cell membrane
Genetics
Metabolism
Mycobacterium tuberculosis
Pathogenicity
Pathology
Ultrastructure
A549 cells
Bacterial proteins
Cell membrane
Humans
Mycobacterium tuberculosis
Tuberculosis vaccines
U937 cells
High activity binding peptide
Mycobacterium tuberculosis h37rv
Ppe7 protein
Synthetic peptide
spellingShingle Bacterial protein
Peptide
Ppe7 protein
Unclassified drug
Bcg vaccine
A-549 cell line
Amino acid sequence
Article
Bacterial gene
Binding affinity
Carboxy terminal sequence
Cell invasion
Cellular distribution
Controlled study
Host cell
Human
Human cell
Immunoelectron microscopy
In vitro study
Macrophage
Microscope
Mycobacterium tuberculosis
Mycobacterium tuberculosis complex
Nonhuman
Protein binding
U-937 cell line
Cell membrane
Genetics
Metabolism
Mycobacterium tuberculosis
Pathogenicity
Pathology
Ultrastructure
A549 cells
Bacterial proteins
Cell membrane
Humans
Mycobacterium tuberculosis
Tuberculosis vaccines
U937 cells
High activity binding peptide
Mycobacterium tuberculosis h37rv
Ppe7 protein
Synthetic peptide
Díaz D.P.
Ocampo M.
Varela Y.
Curtidor H.
Patarroyo M.A.
Patarroyo M.E.
Identifying and characterising PPE7 (Rv0354c) high activity binding peptides and their role in inhibiting cell invasion
description This study was aimed at characterising the PPE7 protein from the PE/PPE protein family. The presence and transcription of the rv0354c gene in the Mycobacterium tuberculosis complex was determined and the subcellular localisation of the PPE7 protein on mycobacterial membrane was confirmed by immunoelectron microscope. Two peptides were identified as having high binding activity (HABPs) and were tested in vitro regarding the invasion of Mycobacterium tuberculosis H37Rv. HABP 39224 inhibited invasion in A549 epithelial cells and U937 macrophages by more than 50%, whilst HABP 39225 inhibited invasion by 40% in U937 cells. HABP 39224, located in the protein’s C-terminal region, has a completely conserved amino acid sequence in M. tuberculosis complex species and could be selected as a base peptide when designing a subunit-based, anti-tuberculosis vaccine. © 2017, Springer Science+Business Media New York.
format Artículo (Article)
author Díaz D.P.
Ocampo M.
Varela Y.
Curtidor H.
Patarroyo M.A.
Patarroyo M.E.
author_facet Díaz D.P.
Ocampo M.
Varela Y.
Curtidor H.
Patarroyo M.A.
Patarroyo M.E.
author_sort Díaz D.P.
title Identifying and characterising PPE7 (Rv0354c) high activity binding peptides and their role in inhibiting cell invasion
title_short Identifying and characterising PPE7 (Rv0354c) high activity binding peptides and their role in inhibiting cell invasion
title_full Identifying and characterising PPE7 (Rv0354c) high activity binding peptides and their role in inhibiting cell invasion
title_fullStr Identifying and characterising PPE7 (Rv0354c) high activity binding peptides and their role in inhibiting cell invasion
title_full_unstemmed Identifying and characterising PPE7 (Rv0354c) high activity binding peptides and their role in inhibiting cell invasion
title_sort identifying and characterising ppe7 (rv0354c) high activity binding peptides and their role in inhibiting cell invasion
publisher Springer New York LLC
publishDate 2017
url https://repository.urosario.edu.co/handle/10336/22603
https://doi.org/10.1007/s11010-017-2962-8
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score 12,131701