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Purification of Trypanosoma cruzi metacyclic trypomastigotes by ion exchange chromatography in sepharose-DEAE, a novel methodology for host-pathogen interaction studies

Metacyclic trypomastigotes are essential for the understanding of the biology of Trypanosoma cruzi, the agent of Chagas disease. However, obtaining these biological stages in axenic medium is difficult. Techniques based on charge and density of the parasite during different stages have been implemen...

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Detalles Bibliográficos
Autores Principales: Cruz-Saavedra L., Muñoz M., León C., Patarroyo M.A., Arevalo G., Pavia P., Vallejo G., Carranza J.C., Ramírez, Juan David
Formato: Artículo (Article)
Lenguaje:Inglés (English)
Publicado: Elsevier B.V. 2017
Materias:
Sepharose
Diethylaminoethyldextran
Animal experiment
Article
Epimastigote
Host pathogen interaction
In vitro study
In vivo study
Ion exchange chromatography
Life cycle
Mouse
Nonhuman
Parasitosis
Priority journal
Trypanosoma cruzi
Trypomastigote
Vero cell line
Animal
Cell line
Chlorocebus aethiops
Institute for cancer research mouse
Isolation and purification
Procedures
Animals
Cercopithecus aethiops
Deae-dextran
Host-pathogen interactions
Mice
Vero cells
Chromatography
Culture
Metacyclic trypomastigotes
Sepharose-deae
inbred icr
ion exchange
Acceso en línea:https://repository.urosario.edu.co/handle/10336/22403
https://doi.org/10.1016/j.mimet.2017.08.021

Internet

https://repository.urosario.edu.co/handle/10336/22403
https://doi.org/10.1016/j.mimet.2017.08.021

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