Comparative study of Candida spp. isolates: Identification and echinocandin susceptibility in isolates obtained from blood cultures in 15 hospitals in Medellín, Colombia

Objectives: Invasive candidiasis has a high impact on morbidity and mortality in hospitalised patients. Accurate and timely methods for identification of Candida spp. and determination of echinocandin susceptibility have become a priority for clinical microbiology laboratories. Methods: This study w...

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Autores Principales: Berrio I., Maldonado N., De Bedout C., Arango K., Cano L.E., Valencia Y., Jiménez-Ortigosa C., Perlin D.S., Gómez B.L., Robledo C., Robledo J., Grupo GERMEN
Formato: Artículo (Article)
Lenguaje:Inglés (English)
Publicado: Elsevier Ltd 2018
Materias:
Acceso en línea:https://repository.urosario.edu.co/handle/10336/22357
https://doi.org/10.1016/j.jgar.2017.11.010
id ir-10336-22357
recordtype dspace
spelling ir-10336-223572022-05-02T12:37:19Z Comparative study of Candida spp. isolates: Identification and echinocandin susceptibility in isolates obtained from blood cultures in 15 hospitals in Medellín, Colombia Berrio I. Maldonado N. De Bedout C. Arango K. Cano L.E. Valencia Y. Jiménez-Ortigosa C. Perlin D.S. Gómez B.L. Robledo C. Robledo J. Grupo GERMEN Anidulafungin Caspofungin Echinocandin Ribosome rna Anidulafungin Antifungal agent Caspofungin Echinocandin Antifungal susceptibility Article Blood culture Broth dilution Candida Candida albicans Candida auris Candida glabrata Candida haemulonii Candida intermedia Candida parapsilosis Candida tropicalis Candidemia Clavispora lusitaniae Colombia Controlled study Dna sequence Epsilometer test Fks1 gene Fks2 gene Fungal gene Fungus identification Gene mutation Gene sequence Matrix assisted laser desorption ionization time of flight mass spectrometry Meyerozyma guilliermondii Minimum inhibitory concentration Nonhuman Pichia kudriavzevii Priority journal Silent mutation Single nucleotide polymorphism Blood culture Candida Candidemia Comparative study Disk diffusion Drug effect Genetics High throughput sequencing Hospital Human Isolation and purification Matrix-assisted laser desorption-ionization mass spectrometry Microbial sensitivity test Rna gene Anidulafungin Antifungal agents Blood culture Candida Candidemia Caspofungin Colombia Disk diffusion antimicrobial tests Echinocandins High-throughput nucleotide sequencing Hospitals Humans Microbial sensitivity tests Candida Candidaemia Echinocandin susceptibility Fks silent mutations Maldi-tof/ms Molecular identification mass rrna matrix-assisted laser desorption-ionization Genes Spectrometry Objectives: Invasive candidiasis has a high impact on morbidity and mortality in hospitalised patients. Accurate and timely methods for identification of Candida spp. and determination of echinocandin susceptibility have become a priority for clinical microbiology laboratories. Methods: This study was performed to compare matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS) identification with sequencing of the D1/D2 region of the rRNA gene complex 28 subunit in 147 Candida spp. isolates obtained from patients with candidaemia. Antimicrobial susceptibility testing was performed by broth microdilution (BMD) and Etest. Sequencing of the FKS1 and FKS2 genes was performed. Results: The most common species isolated were Candida albicans (40.8%), followed by Candida parapsilosis (23.1%) and Candida tropicalis (17.0%). Overall agreement between the results of identification by MALDI-TOF/MS and molecular identification was 99.3%. Anidulafungin and caspofungin susceptibility by the BMD method was 98.0% and 88.4%, respectively. Susceptibility to anidulafungin and caspofungin by Etest was 93.9% and 98.6%, respectively. Categorical agreement between Etest and BMD was 91.8% for anidulafungin and 89.8% for caspofungin, with lower agreements in C. parapsilosis for anidulafungin (76.5%) and C. glabrata for caspofungin (40.0%). No mutations related to resistance were found in the FKS genes, although 54 isolates presented synonymous polymorphisms in the hotspots sequenced. Conclusions: MALDI-TOF/MS is a good alternative for routine identification of Candida spp. isolates. DNA sequencing of the FKS genes suggested that the isolates analysed were susceptible to echinocandins; alternatively, unknown resistance mechanisms or limitations related to antifungal susceptibility tests may explain the resistance found in a few isolates. © 2017 International Society for Chemotherapy of Infection and Cancer 2018 2020-05-25T23:56:12Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 22137165 https://repository.urosario.edu.co/handle/10336/22357 https://doi.org/10.1016/j.jgar.2017.11.010 eng info:eu-repo/semantics/openAccess application/pdf Elsevier Ltd instname:Universidad del Rosario
institution EdocUR - Universidad del Rosario
collection DSpace
language Inglés (English)
topic Anidulafungin
Caspofungin
Echinocandin
Ribosome rna
Anidulafungin
Antifungal agent
Caspofungin
Echinocandin
Antifungal susceptibility
Article
Blood culture
Broth dilution
Candida
Candida albicans
Candida auris
Candida glabrata
Candida haemulonii
Candida intermedia
Candida parapsilosis
Candida tropicalis
Candidemia
Clavispora lusitaniae
Colombia
Controlled study
Dna sequence
Epsilometer test
Fks1 gene
Fks2 gene
Fungal gene
Fungus identification
Gene mutation
Gene sequence
Matrix assisted laser desorption ionization time of flight mass spectrometry
Meyerozyma guilliermondii
Minimum inhibitory concentration
Nonhuman
Pichia kudriavzevii
Priority journal
Silent mutation
Single nucleotide polymorphism
Blood culture
Candida
Candidemia
Comparative study
Disk diffusion
Drug effect
Genetics
High throughput sequencing
Hospital
Human
Isolation and purification
Matrix-assisted laser desorption-ionization mass spectrometry
Microbial sensitivity test
Rna gene
Anidulafungin
Antifungal agents
Blood culture
Candida
Candidemia
Caspofungin
Colombia
Disk diffusion antimicrobial tests
Echinocandins
High-throughput nucleotide sequencing
Hospitals
Humans
Microbial sensitivity tests
Candida
Candidaemia
Echinocandin susceptibility
Fks silent mutations
Maldi-tof/ms
Molecular identification
mass
rrna
matrix-assisted laser desorption-ionization
Genes
Spectrometry
spellingShingle Anidulafungin
Caspofungin
Echinocandin
Ribosome rna
Anidulafungin
Antifungal agent
Caspofungin
Echinocandin
Antifungal susceptibility
Article
Blood culture
Broth dilution
Candida
Candida albicans
Candida auris
Candida glabrata
Candida haemulonii
Candida intermedia
Candida parapsilosis
Candida tropicalis
Candidemia
Clavispora lusitaniae
Colombia
Controlled study
Dna sequence
Epsilometer test
Fks1 gene
Fks2 gene
Fungal gene
Fungus identification
Gene mutation
Gene sequence
Matrix assisted laser desorption ionization time of flight mass spectrometry
Meyerozyma guilliermondii
Minimum inhibitory concentration
Nonhuman
Pichia kudriavzevii
Priority journal
Silent mutation
Single nucleotide polymorphism
Blood culture
Candida
Candidemia
Comparative study
Disk diffusion
Drug effect
Genetics
High throughput sequencing
Hospital
Human
Isolation and purification
Matrix-assisted laser desorption-ionization mass spectrometry
Microbial sensitivity test
Rna gene
Anidulafungin
Antifungal agents
Blood culture
Candida
Candidemia
Caspofungin
Colombia
Disk diffusion antimicrobial tests
Echinocandins
High-throughput nucleotide sequencing
Hospitals
Humans
Microbial sensitivity tests
Candida
Candidaemia
Echinocandin susceptibility
Fks silent mutations
Maldi-tof/ms
Molecular identification
mass
rrna
matrix-assisted laser desorption-ionization
Genes
Spectrometry
Berrio I.
Maldonado N.
De Bedout C.
Arango K.
Cano L.E.
Valencia Y.
Jiménez-Ortigosa C.
Perlin D.S.
Gómez B.L.
Robledo C.
Robledo J.
Grupo GERMEN
Comparative study of Candida spp. isolates: Identification and echinocandin susceptibility in isolates obtained from blood cultures in 15 hospitals in Medellín, Colombia
description Objectives: Invasive candidiasis has a high impact on morbidity and mortality in hospitalised patients. Accurate and timely methods for identification of Candida spp. and determination of echinocandin susceptibility have become a priority for clinical microbiology laboratories. Methods: This study was performed to compare matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS) identification with sequencing of the D1/D2 region of the rRNA gene complex 28 subunit in 147 Candida spp. isolates obtained from patients with candidaemia. Antimicrobial susceptibility testing was performed by broth microdilution (BMD) and Etest. Sequencing of the FKS1 and FKS2 genes was performed. Results: The most common species isolated were Candida albicans (40.8%), followed by Candida parapsilosis (23.1%) and Candida tropicalis (17.0%). Overall agreement between the results of identification by MALDI-TOF/MS and molecular identification was 99.3%. Anidulafungin and caspofungin susceptibility by the BMD method was 98.0% and 88.4%, respectively. Susceptibility to anidulafungin and caspofungin by Etest was 93.9% and 98.6%, respectively. Categorical agreement between Etest and BMD was 91.8% for anidulafungin and 89.8% for caspofungin, with lower agreements in C. parapsilosis for anidulafungin (76.5%) and C. glabrata for caspofungin (40.0%). No mutations related to resistance were found in the FKS genes, although 54 isolates presented synonymous polymorphisms in the hotspots sequenced. Conclusions: MALDI-TOF/MS is a good alternative for routine identification of Candida spp. isolates. DNA sequencing of the FKS genes suggested that the isolates analysed were susceptible to echinocandins; alternatively, unknown resistance mechanisms or limitations related to antifungal susceptibility tests may explain the resistance found in a few isolates. © 2017 International Society for Chemotherapy of Infection and Cancer
format Artículo (Article)
author Berrio I.
Maldonado N.
De Bedout C.
Arango K.
Cano L.E.
Valencia Y.
Jiménez-Ortigosa C.
Perlin D.S.
Gómez B.L.
Robledo C.
Robledo J.
Grupo GERMEN
author_facet Berrio I.
Maldonado N.
De Bedout C.
Arango K.
Cano L.E.
Valencia Y.
Jiménez-Ortigosa C.
Perlin D.S.
Gómez B.L.
Robledo C.
Robledo J.
Grupo GERMEN
author_sort Berrio I.
title Comparative study of Candida spp. isolates: Identification and echinocandin susceptibility in isolates obtained from blood cultures in 15 hospitals in Medellín, Colombia
title_short Comparative study of Candida spp. isolates: Identification and echinocandin susceptibility in isolates obtained from blood cultures in 15 hospitals in Medellín, Colombia
title_full Comparative study of Candida spp. isolates: Identification and echinocandin susceptibility in isolates obtained from blood cultures in 15 hospitals in Medellín, Colombia
title_fullStr Comparative study of Candida spp. isolates: Identification and echinocandin susceptibility in isolates obtained from blood cultures in 15 hospitals in Medellín, Colombia
title_full_unstemmed Comparative study of Candida spp. isolates: Identification and echinocandin susceptibility in isolates obtained from blood cultures in 15 hospitals in Medellín, Colombia
title_sort comparative study of candida spp. isolates: identification and echinocandin susceptibility in isolates obtained from blood cultures in 15 hospitals in medellín, colombia
publisher Elsevier Ltd
publishDate 2018
url https://repository.urosario.edu.co/handle/10336/22357
https://doi.org/10.1016/j.jgar.2017.11.010
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