Heterogeneous genetic diversity pattern in Plasmodium vivax genes encoding merozoite surface proteins (MSP) -7E, -7F and -7L

Background: The msp-7 gene has become differentially expanded in the Plasmodium genus; Plasmodium vivax has the highest copy number of this gene, several of which encode antigenic proteins in merozoites. Methods: DNA sequences from thirty-six Colombian clinical isolates from P. vivax (pv) msp-7E, -7...

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Autores Principales: Garzón-Ospina, Diego, Forero Rodríguez, Johanna, Patarroyo, Manuel A.
Formato: Artículo (Article)
Lenguaje:Inglés (English)
Publicado: 2014
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Acceso en línea:https://repository.urosario.edu.co/handle/10336/21889
https://doi.org/10.1186/1475-2875-13-495
id ir-10336-21889
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spelling ir-10336-218892020-05-13T19:48:55Z Heterogeneous genetic diversity pattern in Plasmodium vivax genes encoding merozoite surface proteins (MSP) -7E, -7F and -7L Garzón-Ospina, Diego Forero Rodríguez, Johanna Patarroyo, Manuel A. Enfermedades Evolución & genética Plasmodium vivax msp-7 Genetic diversity Natural selection Selective sweep Background: The msp-7 gene has become differentially expanded in the Plasmodium genus; Plasmodium vivax has the highest copy number of this gene, several of which encode antigenic proteins in merozoites. Methods: DNA sequences from thirty-six Colombian clinical isolates from P. vivax (pv) msp-7E, -7F and -7L genes were analysed for characterizing and studying the genetic diversity of these pvmsp-7 members which are expressed during the intra-erythrocyte stage; natural selection signals producing the variation pattern so observed were evaluated. Results: The pvmsp-7E gene was highly polymorphic compared to pvmsp-7F and pvmsp-7L which were seen to have limited genetic diversity; pvmsp-7E polymorphism was seen to have been maintained by different types of positive selection. Even though these copies seemed to be species-specific duplications, a search in the Plasmodium cynomolgi genome (P. vivax sister taxon) showed that both species shared the whole msp-7 repertoire. This led to exploring the long-term effect of natural selection by comparing the orthologous sequences which led to finding signatures for lineage-specific positive selection. Conclusions: The results confirmed that the P. vivax msp-7 family has a heterogeneous genetic diversity pattern; some members are highly conserved whilst others are highly diverse. The results suggested that the 3′-end of these genes encode MSP-7 proteins' functional region whilst the central region of pvmsp-7E has evolved rapidly. The lineage-specific positive selection signals found suggested that mutations occurring in msp-7s genes during host switch may have succeeded in adapting the ancestral P. vivax parasite population to humans. © 2014 Garzón-Ospina et al. 2014 2020-05-07T11:29:33Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 1475-2875 https://repository.urosario.edu.co/handle/10336/21889 https://doi.org/10.1186/1475-2875-13-495 eng info:eu-repo/semantics/openAccess application/pdf
institution EdocUR - Universidad del Rosario
collection DSpace
language Inglés (English)
topic Enfermedades
Evolución & genética
Plasmodium vivax
msp-7
Genetic diversity
Natural selection
Selective sweep
spellingShingle Enfermedades
Evolución & genética
Plasmodium vivax
msp-7
Genetic diversity
Natural selection
Selective sweep
Garzón-Ospina, Diego
Forero Rodríguez, Johanna
Patarroyo, Manuel A.
Heterogeneous genetic diversity pattern in Plasmodium vivax genes encoding merozoite surface proteins (MSP) -7E, -7F and -7L
description Background: The msp-7 gene has become differentially expanded in the Plasmodium genus; Plasmodium vivax has the highest copy number of this gene, several of which encode antigenic proteins in merozoites. Methods: DNA sequences from thirty-six Colombian clinical isolates from P. vivax (pv) msp-7E, -7F and -7L genes were analysed for characterizing and studying the genetic diversity of these pvmsp-7 members which are expressed during the intra-erythrocyte stage; natural selection signals producing the variation pattern so observed were evaluated. Results: The pvmsp-7E gene was highly polymorphic compared to pvmsp-7F and pvmsp-7L which were seen to have limited genetic diversity; pvmsp-7E polymorphism was seen to have been maintained by different types of positive selection. Even though these copies seemed to be species-specific duplications, a search in the Plasmodium cynomolgi genome (P. vivax sister taxon) showed that both species shared the whole msp-7 repertoire. This led to exploring the long-term effect of natural selection by comparing the orthologous sequences which led to finding signatures for lineage-specific positive selection. Conclusions: The results confirmed that the P. vivax msp-7 family has a heterogeneous genetic diversity pattern; some members are highly conserved whilst others are highly diverse. The results suggested that the 3′-end of these genes encode MSP-7 proteins' functional region whilst the central region of pvmsp-7E has evolved rapidly. The lineage-specific positive selection signals found suggested that mutations occurring in msp-7s genes during host switch may have succeeded in adapting the ancestral P. vivax parasite population to humans. © 2014 Garzón-Ospina et al.
format Artículo (Article)
author Garzón-Ospina, Diego
Forero Rodríguez, Johanna
Patarroyo, Manuel A.
author_facet Garzón-Ospina, Diego
Forero Rodríguez, Johanna
Patarroyo, Manuel A.
author_sort Garzón-Ospina, Diego
title Heterogeneous genetic diversity pattern in Plasmodium vivax genes encoding merozoite surface proteins (MSP) -7E, -7F and -7L
title_short Heterogeneous genetic diversity pattern in Plasmodium vivax genes encoding merozoite surface proteins (MSP) -7E, -7F and -7L
title_full Heterogeneous genetic diversity pattern in Plasmodium vivax genes encoding merozoite surface proteins (MSP) -7E, -7F and -7L
title_fullStr Heterogeneous genetic diversity pattern in Plasmodium vivax genes encoding merozoite surface proteins (MSP) -7E, -7F and -7L
title_full_unstemmed Heterogeneous genetic diversity pattern in Plasmodium vivax genes encoding merozoite surface proteins (MSP) -7E, -7F and -7L
title_sort heterogeneous genetic diversity pattern in plasmodium vivax genes encoding merozoite surface proteins (msp) -7e, -7f and -7l
publishDate 2014
url https://repository.urosario.edu.co/handle/10336/21889
https://doi.org/10.1186/1475-2875-13-495
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score 12,131701