Molecular epidemiology of Giardia, Blastocystis and Cryptosporidium among Indigenous children from the Colombian Amazon basin

The incidence and prevalence of intestinal parasites in children is most likely due to lack of natural or acquired resistance and differences in behavior and habits closely related to environmental and socioeconomic determinants. The most important protozoa that parasitize humans are Giardia, Entamo...

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Autores Principales: Sánchez, Angie, Munoz, Marina, Gómez, Natalia, Tabares, Juan, Segura, Laura, Salazar, Ángela, Restrepo, Cristian, Ruíz, Miguel, Reyes, Patricia, Qian, Yuchen, Xiao, Lihua, López C., Myriam, Ramírez, Juan David
Formato: Artículo (Article)
Lenguaje:Inglés (English)
Publicado: 2017
Materias:
Acceso en línea:https://repository.urosario.edu.co/handle/10336/21758
https://doi.org/10.3389/fmicb.2017.00248
id ir-10336-21758
recordtype dspace
spelling ir-10336-217582020-05-13T19:49:44Z Molecular epidemiology of Giardia, Blastocystis and Cryptosporidium among Indigenous children from the Colombian Amazon basin Sánchez, Angie Munoz, Marina Gómez, Natalia Tabares, Juan Segura, Laura Salazar, Ángela Restrepo, Cristian Ruíz, Miguel Reyes, Patricia Qian, Yuchen Xiao, Lihua López C., Myriam Ramírez, Juan David Enfermedades Blastocystis Blastocystosis Child Controlled study Cryptosporidiosis Cryptosporidium DNA extraction Genetic variability Giardia Giardiasis Human Intestine parasite Microscopy Nucleotide sequence Prevalence Real time polymerase chain reaction The incidence and prevalence of intestinal parasites in children is most likely due to lack of natural or acquired resistance and differences in behavior and habits closely related to environmental and socioeconomic determinants. The most important protozoa that parasitize humans are Giardia, Entamoeba, Blastocystis, and Cryptosporidium. These parasites present wide intraspecific genetic diversity and subsequently classified into assemblages and subtypes. The Amazon basin is the largest in the world and is the fifth freshwater reserve on the planet. Contradictorily, people living in these areas (Indigenous populations) have poor quality of life, which favors the infection of diseases of fecal-oral transmission. The aim of this work was to unravel the molecular epidemiology of Giardia, Blastocystis and Cryptosporidium across four communities (Puerto Nariño, San Juan del Soco, Villa Andrea and Nuevo Paraíso). We obtained 284 fecal samples from children under 15 years old that were analyzed by direct microscopy (261 samples) and Real Time PCR (qPCR) (284 samples). The positive samples for these protozoa were further characterized by several molecular markers to depict assemblages and subtypes. We observed a frequency of Giardia infection by microscopy of 23.7% (62 samples) and by qPCR of 64.8% (184 samples); for Blastocystis by microscopy of 35.2% (92 samples) and by qPCR of 88.7% (252 samples) and for Cryptosporidium only 1.9% (5 samples) were positive by microscopy and qPCR 1.8% (5 samples). Regarding the Giardia assemblages, using the glutamate dehydrogenase (gdh) marker we observed AI, BIII and BIV assemblages and when using triose phosphate isomerase (tpi) we observed assemblages AI, AII, BIII and BIV. In contrast, Blastocystis STs detected were 1, 2, 3, 4, and 6. Lastly, the species C. viatorum, C. hominis (with the subtypes IdA19 and IaA12R8) and C. parvum (with the subtype IIcA5G3c) were identified. We observed a high profile of zoonotic transmission regarding the Giardia assemblages and Blastocystis STs/alleles. Also, we highlight the elevated frequency of infection by these two protozoans suggesting an active transmission in the area. Our findings reinforces the need to deploy better epidemiological surveillance systems for enteric pathogens in the area. © 2017 Sánchez, Munoz, Gómez, Tabares, Segura, Salazar, Restrepo, Ruíz, Reyes, Qian, Xiao, López and Ramírez. 2017 2020-04-24T01:17:46Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 1664-302X https://repository.urosario.edu.co/handle/10336/21758 https://doi.org/10.3389/fmicb.2017.00248 eng info:eu-repo/semantics/openAccess application/pdf
institution EdocUR - Universidad del Rosario
collection DSpace
language Inglés (English)
topic Enfermedades
Blastocystis
Blastocystosis
Child
Controlled study
Cryptosporidiosis
Cryptosporidium
DNA extraction
Genetic variability
Giardia
Giardiasis
Human
Intestine parasite
Microscopy
Nucleotide sequence
Prevalence
Real time polymerase chain reaction
spellingShingle Enfermedades
Blastocystis
Blastocystosis
Child
Controlled study
Cryptosporidiosis
Cryptosporidium
DNA extraction
Genetic variability
Giardia
Giardiasis
Human
Intestine parasite
Microscopy
Nucleotide sequence
Prevalence
Real time polymerase chain reaction
Sánchez, Angie
Munoz, Marina
Gómez, Natalia
Tabares, Juan
Segura, Laura
Salazar, Ángela
Restrepo, Cristian
Ruíz, Miguel
Reyes, Patricia
Qian, Yuchen
Xiao, Lihua
López C., Myriam
Ramírez, Juan David
Molecular epidemiology of Giardia, Blastocystis and Cryptosporidium among Indigenous children from the Colombian Amazon basin
description The incidence and prevalence of intestinal parasites in children is most likely due to lack of natural or acquired resistance and differences in behavior and habits closely related to environmental and socioeconomic determinants. The most important protozoa that parasitize humans are Giardia, Entamoeba, Blastocystis, and Cryptosporidium. These parasites present wide intraspecific genetic diversity and subsequently classified into assemblages and subtypes. The Amazon basin is the largest in the world and is the fifth freshwater reserve on the planet. Contradictorily, people living in these areas (Indigenous populations) have poor quality of life, which favors the infection of diseases of fecal-oral transmission. The aim of this work was to unravel the molecular epidemiology of Giardia, Blastocystis and Cryptosporidium across four communities (Puerto Nariño, San Juan del Soco, Villa Andrea and Nuevo Paraíso). We obtained 284 fecal samples from children under 15 years old that were analyzed by direct microscopy (261 samples) and Real Time PCR (qPCR) (284 samples). The positive samples for these protozoa were further characterized by several molecular markers to depict assemblages and subtypes. We observed a frequency of Giardia infection by microscopy of 23.7% (62 samples) and by qPCR of 64.8% (184 samples); for Blastocystis by microscopy of 35.2% (92 samples) and by qPCR of 88.7% (252 samples) and for Cryptosporidium only 1.9% (5 samples) were positive by microscopy and qPCR 1.8% (5 samples). Regarding the Giardia assemblages, using the glutamate dehydrogenase (gdh) marker we observed AI, BIII and BIV assemblages and when using triose phosphate isomerase (tpi) we observed assemblages AI, AII, BIII and BIV. In contrast, Blastocystis STs detected were 1, 2, 3, 4, and 6. Lastly, the species C. viatorum, C. hominis (with the subtypes IdA19 and IaA12R8) and C. parvum (with the subtype IIcA5G3c) were identified. We observed a high profile of zoonotic transmission regarding the Giardia assemblages and Blastocystis STs/alleles. Also, we highlight the elevated frequency of infection by these two protozoans suggesting an active transmission in the area. Our findings reinforces the need to deploy better epidemiological surveillance systems for enteric pathogens in the area. © 2017 Sánchez, Munoz, Gómez, Tabares, Segura, Salazar, Restrepo, Ruíz, Reyes, Qian, Xiao, López and Ramírez.
format Artículo (Article)
author Sánchez, Angie
Munoz, Marina
Gómez, Natalia
Tabares, Juan
Segura, Laura
Salazar, Ángela
Restrepo, Cristian
Ruíz, Miguel
Reyes, Patricia
Qian, Yuchen
Xiao, Lihua
López C., Myriam
Ramírez, Juan David
author_facet Sánchez, Angie
Munoz, Marina
Gómez, Natalia
Tabares, Juan
Segura, Laura
Salazar, Ángela
Restrepo, Cristian
Ruíz, Miguel
Reyes, Patricia
Qian, Yuchen
Xiao, Lihua
López C., Myriam
Ramírez, Juan David
author_sort Sánchez, Angie
title Molecular epidemiology of Giardia, Blastocystis and Cryptosporidium among Indigenous children from the Colombian Amazon basin
title_short Molecular epidemiology of Giardia, Blastocystis and Cryptosporidium among Indigenous children from the Colombian Amazon basin
title_full Molecular epidemiology of Giardia, Blastocystis and Cryptosporidium among Indigenous children from the Colombian Amazon basin
title_fullStr Molecular epidemiology of Giardia, Blastocystis and Cryptosporidium among Indigenous children from the Colombian Amazon basin
title_full_unstemmed Molecular epidemiology of Giardia, Blastocystis and Cryptosporidium among Indigenous children from the Colombian Amazon basin
title_sort molecular epidemiology of giardia, blastocystis and cryptosporidium among indigenous children from the colombian amazon basin
publishDate 2017
url https://repository.urosario.edu.co/handle/10336/21758
https://doi.org/10.3389/fmicb.2017.00248
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