Whole-genome sequencing to determine origin of multinational outbreak of Sarocladium kiliense bloodstream infections
We used whole-genome sequence typing (WGST) to investigate an outbreak of Sarocladium kiliense bloodstream infections (BSI) associated with receipt of contaminated antinausea medication among oncology patients in Colombia and Chile during 2013-2014. Twenty-five outbreak isolates (18 from patients an...
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Acceso en línea: | https://repository.urosario.edu.co/handle/10336/21348 https://doi.org/10.3201/eid2203.151193 |
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ir-10336-213482020-05-13T19:48:30Z Whole-genome sequencing to determine origin of multinational outbreak of Sarocladium kiliense bloodstream infections Etienne, Kizee A. Roe, Chandler C. Smith, Rachel M. Vallabhaneni, Snigdha Duarte-Rey, Carolina Escandón, Patricia Castañeda, Elizabeth Gómez, Beatriz L. Bedout, Catalina de López, Luisa F. Salas, Valentina Hederra, Luz Maria Fernández, Jorge Pidal, Paola Hormazabel, Juan Carlos Otaíza-O’Ryan, Fernando Vannberg, Fredrik O. Gillece, John Lemmer, Darrin Driebe, Elizabeth M. Engelthaler, David M. Litvintseva, Anastasia P. Infección del torrente sanguíneo Artículo clínico Estudio controlado Secuencia génica Análisis del genoma humano Filogenia Infección del torrente sanguíneo de Sarocladium kiliense Polimorfismo de nucleótido simple Evolución & genética Técnicas genéticas Agentes infecciosos Medicamentos Oncologías Bloodstream infection Clinical article Controlled study Gene sequence Genome analysis Phylogeny Sarocladium kiliense bloodstream infection Single nucleotide polymorphism We used whole-genome sequence typing (WGST) to investigate an outbreak of Sarocladium kiliense bloodstream infections (BSI) associated with receipt of contaminated antinausea medication among oncology patients in Colombia and Chile during 2013-2014. Twenty-five outbreak isolates (18 from patients and 7 from medication vials) and 11 control isolates unrelated to this outbreak were subjected to WGST to elucidate a source of infection. All outbreak isolates were nearly indistinguishable (≤5 single-nucleotide polymorphisms), and >21,000 single-nucleotide polymorphisms were identified from unrelated control isolates, suggesting a point source for this outbreak. S. kiliense has been previously implicated in healthcare-related infections; however, the lack of available typing methods has precluded the ability to substantiate point sources. WGST for outbreak investigation caused by eukaryotic pathogens without reference genomes or existing genotyping methods enables accurate source identification to guide implementation of appropriate control and prevention measures. © 2016, Centers for Disease Control and Prevention (CDC). All rights reserved. 2016-03 2020-04-01T14:51:46Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 1080-6040 https://repository.urosario.edu.co/handle/10336/21348 https://doi.org/10.3201/eid2203.151193 eng info:eu-repo/semantics/openAccess application/pdf |
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EdocUR - Universidad del Rosario |
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language |
Inglés (English) |
topic |
Infección del torrente sanguíneo Artículo clínico Estudio controlado Secuencia génica Análisis del genoma humano Filogenia Infección del torrente sanguíneo de Sarocladium kiliense Polimorfismo de nucleótido simple Evolución & genética Técnicas genéticas Agentes infecciosos Medicamentos Oncologías Bloodstream infection Clinical article Controlled study Gene sequence Genome analysis Phylogeny Sarocladium kiliense bloodstream infection Single nucleotide polymorphism |
spellingShingle |
Infección del torrente sanguíneo Artículo clínico Estudio controlado Secuencia génica Análisis del genoma humano Filogenia Infección del torrente sanguíneo de Sarocladium kiliense Polimorfismo de nucleótido simple Evolución & genética Técnicas genéticas Agentes infecciosos Medicamentos Oncologías Bloodstream infection Clinical article Controlled study Gene sequence Genome analysis Phylogeny Sarocladium kiliense bloodstream infection Single nucleotide polymorphism Etienne, Kizee A. Roe, Chandler C. Smith, Rachel M. Vallabhaneni, Snigdha Duarte-Rey, Carolina Escandón, Patricia Castañeda, Elizabeth Gómez, Beatriz L. Bedout, Catalina de López, Luisa F. Salas, Valentina Hederra, Luz Maria Fernández, Jorge Pidal, Paola Hormazabel, Juan Carlos Otaíza-O’Ryan, Fernando Vannberg, Fredrik O. Gillece, John Lemmer, Darrin Driebe, Elizabeth M. Engelthaler, David M. Litvintseva, Anastasia P. Whole-genome sequencing to determine origin of multinational outbreak of Sarocladium kiliense bloodstream infections |
description |
We used whole-genome sequence typing (WGST) to investigate an outbreak of Sarocladium kiliense bloodstream infections (BSI) associated with receipt of contaminated antinausea medication among oncology patients in Colombia and Chile during 2013-2014. Twenty-five outbreak isolates (18 from patients and 7 from medication vials) and 11 control isolates unrelated to this outbreak were subjected to WGST to elucidate a source of infection. All outbreak isolates were nearly indistinguishable (≤5 single-nucleotide polymorphisms), and >21,000 single-nucleotide polymorphisms were identified from unrelated control isolates, suggesting a point source for this outbreak. S. kiliense has been previously implicated in healthcare-related infections; however, the lack of available typing methods has precluded the ability to substantiate point sources. WGST for outbreak investigation caused by eukaryotic pathogens without reference genomes or existing genotyping methods enables accurate source identification to guide implementation of appropriate control and prevention measures. © 2016, Centers for Disease Control and Prevention (CDC). All rights reserved. |
format |
Artículo (Article) |
author |
Etienne, Kizee A. Roe, Chandler C. Smith, Rachel M. Vallabhaneni, Snigdha Duarte-Rey, Carolina Escandón, Patricia Castañeda, Elizabeth Gómez, Beatriz L. Bedout, Catalina de López, Luisa F. Salas, Valentina Hederra, Luz Maria Fernández, Jorge Pidal, Paola Hormazabel, Juan Carlos Otaíza-O’Ryan, Fernando Vannberg, Fredrik O. Gillece, John Lemmer, Darrin Driebe, Elizabeth M. Engelthaler, David M. Litvintseva, Anastasia P. |
author_facet |
Etienne, Kizee A. Roe, Chandler C. Smith, Rachel M. Vallabhaneni, Snigdha Duarte-Rey, Carolina Escandón, Patricia Castañeda, Elizabeth Gómez, Beatriz L. Bedout, Catalina de López, Luisa F. Salas, Valentina Hederra, Luz Maria Fernández, Jorge Pidal, Paola Hormazabel, Juan Carlos Otaíza-O’Ryan, Fernando Vannberg, Fredrik O. Gillece, John Lemmer, Darrin Driebe, Elizabeth M. Engelthaler, David M. Litvintseva, Anastasia P. |
author_sort |
Etienne, Kizee A. |
title |
Whole-genome sequencing to determine origin of multinational outbreak of Sarocladium kiliense bloodstream infections |
title_short |
Whole-genome sequencing to determine origin of multinational outbreak of Sarocladium kiliense bloodstream infections |
title_full |
Whole-genome sequencing to determine origin of multinational outbreak of Sarocladium kiliense bloodstream infections |
title_fullStr |
Whole-genome sequencing to determine origin of multinational outbreak of Sarocladium kiliense bloodstream infections |
title_full_unstemmed |
Whole-genome sequencing to determine origin of multinational outbreak of Sarocladium kiliense bloodstream infections |
title_sort |
whole-genome sequencing to determine origin of multinational outbreak of sarocladium kiliense bloodstream infections |
publishDate |
2016 |
url |
https://repository.urosario.edu.co/handle/10336/21348 https://doi.org/10.3201/eid2203.151193 |
_version_ |
1667195704364236800 |
score |
12,131701 |