Whole-genome sequencing to determine origin of multinational outbreak of Sarocladium kiliense bloodstream infections

We used whole-genome sequence typing (WGST) to investigate an outbreak of Sarocladium kiliense bloodstream infections (BSI) associated with receipt of contaminated antinausea medication among oncology patients in Colombia and Chile during 2013-2014. Twenty-five outbreak isolates (18 from patients an...

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Autores Principales: Etienne, Kizee A., Roe, Chandler C., Smith, Rachel M., Vallabhaneni, Snigdha, Duarte-Rey, Carolina, Escandón, Patricia, Castañeda, Elizabeth, Gómez, Beatriz L., Bedout, Catalina de, López, Luisa F., Salas, Valentina, Hederra, Luz Maria, Fernández, Jorge, Pidal, Paola, Hormazabel, Juan Carlos, Otaíza-O’Ryan, Fernando, Vannberg, Fredrik O., Gillece, John, Lemmer, Darrin, Driebe, Elizabeth M., Engelthaler, David M., Litvintseva, Anastasia P.
Formato: Artículo (Article)
Lenguaje:Inglés (English)
Publicado: 2016
Materias:
Acceso en línea:https://repository.urosario.edu.co/handle/10336/21348
https://doi.org/10.3201/eid2203.151193
id ir-10336-21348
recordtype dspace
spelling ir-10336-213482020-05-13T19:48:30Z Whole-genome sequencing to determine origin of multinational outbreak of Sarocladium kiliense bloodstream infections Etienne, Kizee A. Roe, Chandler C. Smith, Rachel M. Vallabhaneni, Snigdha Duarte-Rey, Carolina Escandón, Patricia Castañeda, Elizabeth Gómez, Beatriz L. Bedout, Catalina de López, Luisa F. Salas, Valentina Hederra, Luz Maria Fernández, Jorge Pidal, Paola Hormazabel, Juan Carlos Otaíza-O’Ryan, Fernando Vannberg, Fredrik O. Gillece, John Lemmer, Darrin Driebe, Elizabeth M. Engelthaler, David M. Litvintseva, Anastasia P. Infección del torrente sanguíneo Artículo clínico Estudio controlado Secuencia génica Análisis del genoma humano Filogenia Infección del torrente sanguíneo de Sarocladium kiliense Polimorfismo de nucleótido simple Evolución & genética Técnicas genéticas Agentes infecciosos Medicamentos Oncologías Bloodstream infection Clinical article Controlled study Gene sequence Genome analysis Phylogeny Sarocladium kiliense bloodstream infection Single nucleotide polymorphism We used whole-genome sequence typing (WGST) to investigate an outbreak of Sarocladium kiliense bloodstream infections (BSI) associated with receipt of contaminated antinausea medication among oncology patients in Colombia and Chile during 2013-2014. Twenty-five outbreak isolates (18 from patients and 7 from medication vials) and 11 control isolates unrelated to this outbreak were subjected to WGST to elucidate a source of infection. All outbreak isolates were nearly indistinguishable (≤5 single-nucleotide polymorphisms), and >21,000 single-nucleotide polymorphisms were identified from unrelated control isolates, suggesting a point source for this outbreak. S. kiliense has been previously implicated in healthcare-related infections; however, the lack of available typing methods has precluded the ability to substantiate point sources. WGST for outbreak investigation caused by eukaryotic pathogens without reference genomes or existing genotyping methods enables accurate source identification to guide implementation of appropriate control and prevention measures. © 2016, Centers for Disease Control and Prevention (CDC). All rights reserved. 2016-03 2020-04-01T14:51:46Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 1080-6040 https://repository.urosario.edu.co/handle/10336/21348 https://doi.org/10.3201/eid2203.151193 eng info:eu-repo/semantics/openAccess application/pdf
institution EdocUR - Universidad del Rosario
collection DSpace
language Inglés (English)
topic Infección del torrente sanguíneo
Artículo clínico
Estudio controlado
Secuencia génica
Análisis del genoma humano
Filogenia Infección del torrente sanguíneo de Sarocladium kiliense
Polimorfismo de nucleótido simple
Evolución & genética
Técnicas genéticas
Agentes infecciosos
Medicamentos
Oncologías
Bloodstream infection
Clinical article
Controlled study
Gene sequence
Genome analysis
Phylogeny
Sarocladium kiliense bloodstream infection
Single nucleotide polymorphism
spellingShingle Infección del torrente sanguíneo
Artículo clínico
Estudio controlado
Secuencia génica
Análisis del genoma humano
Filogenia Infección del torrente sanguíneo de Sarocladium kiliense
Polimorfismo de nucleótido simple
Evolución & genética
Técnicas genéticas
Agentes infecciosos
Medicamentos
Oncologías
Bloodstream infection
Clinical article
Controlled study
Gene sequence
Genome analysis
Phylogeny
Sarocladium kiliense bloodstream infection
Single nucleotide polymorphism
Etienne, Kizee A.
Roe, Chandler C.
Smith, Rachel M.
Vallabhaneni, Snigdha
Duarte-Rey, Carolina
Escandón, Patricia
Castañeda, Elizabeth
Gómez, Beatriz L.
Bedout, Catalina de
López, Luisa F.
Salas, Valentina
Hederra, Luz Maria
Fernández, Jorge
Pidal, Paola
Hormazabel, Juan Carlos
Otaíza-O’Ryan, Fernando
Vannberg, Fredrik O.
Gillece, John
Lemmer, Darrin
Driebe, Elizabeth M.
Engelthaler, David M.
Litvintseva, Anastasia P.
Whole-genome sequencing to determine origin of multinational outbreak of Sarocladium kiliense bloodstream infections
description We used whole-genome sequence typing (WGST) to investigate an outbreak of Sarocladium kiliense bloodstream infections (BSI) associated with receipt of contaminated antinausea medication among oncology patients in Colombia and Chile during 2013-2014. Twenty-five outbreak isolates (18 from patients and 7 from medication vials) and 11 control isolates unrelated to this outbreak were subjected to WGST to elucidate a source of infection. All outbreak isolates were nearly indistinguishable (≤5 single-nucleotide polymorphisms), and >21,000 single-nucleotide polymorphisms were identified from unrelated control isolates, suggesting a point source for this outbreak. S. kiliense has been previously implicated in healthcare-related infections; however, the lack of available typing methods has precluded the ability to substantiate point sources. WGST for outbreak investigation caused by eukaryotic pathogens without reference genomes or existing genotyping methods enables accurate source identification to guide implementation of appropriate control and prevention measures. © 2016, Centers for Disease Control and Prevention (CDC). All rights reserved.
format Artículo (Article)
author Etienne, Kizee A.
Roe, Chandler C.
Smith, Rachel M.
Vallabhaneni, Snigdha
Duarte-Rey, Carolina
Escandón, Patricia
Castañeda, Elizabeth
Gómez, Beatriz L.
Bedout, Catalina de
López, Luisa F.
Salas, Valentina
Hederra, Luz Maria
Fernández, Jorge
Pidal, Paola
Hormazabel, Juan Carlos
Otaíza-O’Ryan, Fernando
Vannberg, Fredrik O.
Gillece, John
Lemmer, Darrin
Driebe, Elizabeth M.
Engelthaler, David M.
Litvintseva, Anastasia P.
author_facet Etienne, Kizee A.
Roe, Chandler C.
Smith, Rachel M.
Vallabhaneni, Snigdha
Duarte-Rey, Carolina
Escandón, Patricia
Castañeda, Elizabeth
Gómez, Beatriz L.
Bedout, Catalina de
López, Luisa F.
Salas, Valentina
Hederra, Luz Maria
Fernández, Jorge
Pidal, Paola
Hormazabel, Juan Carlos
Otaíza-O’Ryan, Fernando
Vannberg, Fredrik O.
Gillece, John
Lemmer, Darrin
Driebe, Elizabeth M.
Engelthaler, David M.
Litvintseva, Anastasia P.
author_sort Etienne, Kizee A.
title Whole-genome sequencing to determine origin of multinational outbreak of Sarocladium kiliense bloodstream infections
title_short Whole-genome sequencing to determine origin of multinational outbreak of Sarocladium kiliense bloodstream infections
title_full Whole-genome sequencing to determine origin of multinational outbreak of Sarocladium kiliense bloodstream infections
title_fullStr Whole-genome sequencing to determine origin of multinational outbreak of Sarocladium kiliense bloodstream infections
title_full_unstemmed Whole-genome sequencing to determine origin of multinational outbreak of Sarocladium kiliense bloodstream infections
title_sort whole-genome sequencing to determine origin of multinational outbreak of sarocladium kiliense bloodstream infections
publishDate 2016
url https://repository.urosario.edu.co/handle/10336/21348
https://doi.org/10.3201/eid2203.151193
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