Evaluation of a Multilocus Sequence Typing (MLST) scheme for Leishmania (Viannia) braziliensis and Leishmania (Viannia) panamensis in Colombia

BACKGROUND: Leishmaniases are parasitic vector-borne diseases affecting more than 12 million people in 98 countries. In Colombia, leishmaniasis is widespread and the most common clinical manifestation is cutaneous, mainly caused by L. panamensis and L. braziliensis. Currently, the genetic diversity...

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Autores Principales: Herrera, Giovanny, Hernández, Carolina, Ayala, Martha.S., Flórez, Carolina, Teherán, Aníbal A., Ramírez, Juan David
Formato: Artículo (Article)
Lenguaje:Inglés (English)
Publicado: 2017
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Acceso en línea:http://repository.urosario.edu.co/handle/10336/19204
id ir-10336-19204
recordtype dspace
spelling ir-10336-192042019-09-19T12:37:54Z Evaluation of a Multilocus Sequence Typing (MLST) scheme for Leishmania (Viannia) braziliensis and Leishmania (Viannia) panamensis in Colombia Herrera, Giovanny Hernández, Carolina Ayala, Martha.S. Flórez, Carolina Teherán, Aníbal A. Ramírez, Juan David Leishmania MLST DST Cutaneous leishmaniasis Genetic diversity Enfermedades Leishmaniasis Infecciones por protozoarios Variación genética BACKGROUND: Leishmaniases are parasitic vector-borne diseases affecting more than 12 million people in 98 countries. In Colombia, leishmaniasis is widespread and the most common clinical manifestation is cutaneous, mainly caused by L. panamensis and L. braziliensis. Currently, the genetic diversity of these species in Colombia is unknown. To address this, we applied molecular techniques for their characterization, using multilocus sequence typing (MLST) to explore the genetic variability and phylodynamics of the disease.METHODS: Seven previously described genetic markers were selected highlighting the implementation of a mitochondrial marker. Markers were applied to 163 samples from isolates obtained between 1980 and 2001.RESULTS: The identification of the samples showed an excellent correlation with typing tests previously applied (MLEE, monoclonal antibodies). Isolates of L. braziliensis showed greater genetic diversity than L. panamensis, and a greater number of diploid sequence types (DSTs). In addition, the geographical distribution of DSTs for each species were obtained through georeferencing maps.CONCLUSIONS: To our knowldge, this study represents the first description of the genetic variability of L. panamensis in Colombia and South America, and is the first to propose a scheme of MLST for epidemiological surveillance of leishmaniasis in the country. 2017 2019-03-06T17:46:28Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 1756-3305 http://repository.urosario.edu.co/handle/10336/19204 10.1186/s13071-017-2175-8 eng info:eu-repo/semantics/openAccess application/pdf
institution EdocUR - Universidad del Rosario
collection DSpace
language Inglés (English)
topic Leishmania
MLST
DST
Cutaneous
leishmaniasis
Genetic
diversity
Enfermedades
Leishmaniasis
Infecciones por protozoarios
Variación genética
spellingShingle Leishmania
MLST
DST
Cutaneous
leishmaniasis
Genetic
diversity
Enfermedades
Leishmaniasis
Infecciones por protozoarios
Variación genética
Herrera, Giovanny
Hernández, Carolina
Ayala, Martha.S.
Flórez, Carolina
Teherán, Aníbal A.
Ramírez, Juan David
Evaluation of a Multilocus Sequence Typing (MLST) scheme for Leishmania (Viannia) braziliensis and Leishmania (Viannia) panamensis in Colombia
description BACKGROUND: Leishmaniases are parasitic vector-borne diseases affecting more than 12 million people in 98 countries. In Colombia, leishmaniasis is widespread and the most common clinical manifestation is cutaneous, mainly caused by L. panamensis and L. braziliensis. Currently, the genetic diversity of these species in Colombia is unknown. To address this, we applied molecular techniques for their characterization, using multilocus sequence typing (MLST) to explore the genetic variability and phylodynamics of the disease.METHODS: Seven previously described genetic markers were selected highlighting the implementation of a mitochondrial marker. Markers were applied to 163 samples from isolates obtained between 1980 and 2001.RESULTS: The identification of the samples showed an excellent correlation with typing tests previously applied (MLEE, monoclonal antibodies). Isolates of L. braziliensis showed greater genetic diversity than L. panamensis, and a greater number of diploid sequence types (DSTs). In addition, the geographical distribution of DSTs for each species were obtained through georeferencing maps.CONCLUSIONS: To our knowldge, this study represents the first description of the genetic variability of L. panamensis in Colombia and South America, and is the first to propose a scheme of MLST for epidemiological surveillance of leishmaniasis in the country.
format Artículo (Article)
author Herrera, Giovanny
Hernández, Carolina
Ayala, Martha.S.
Flórez, Carolina
Teherán, Aníbal A.
Ramírez, Juan David
author_facet Herrera, Giovanny
Hernández, Carolina
Ayala, Martha.S.
Flórez, Carolina
Teherán, Aníbal A.
Ramírez, Juan David
author_sort Herrera, Giovanny
title Evaluation of a Multilocus Sequence Typing (MLST) scheme for Leishmania (Viannia) braziliensis and Leishmania (Viannia) panamensis in Colombia
title_short Evaluation of a Multilocus Sequence Typing (MLST) scheme for Leishmania (Viannia) braziliensis and Leishmania (Viannia) panamensis in Colombia
title_full Evaluation of a Multilocus Sequence Typing (MLST) scheme for Leishmania (Viannia) braziliensis and Leishmania (Viannia) panamensis in Colombia
title_fullStr Evaluation of a Multilocus Sequence Typing (MLST) scheme for Leishmania (Viannia) braziliensis and Leishmania (Viannia) panamensis in Colombia
title_full_unstemmed Evaluation of a Multilocus Sequence Typing (MLST) scheme for Leishmania (Viannia) braziliensis and Leishmania (Viannia) panamensis in Colombia
title_sort evaluation of a multilocus sequence typing (mlst) scheme for leishmania (viannia) braziliensis and leishmania (viannia) panamensis in colombia
publishDate 2017
url http://repository.urosario.edu.co/handle/10336/19204
_version_ 1645141997748486144
score 12,131701