IMPIPS : The Immune Protection-Inducing Protein Structure concept in the search for steric-electron and topochemical principles for complete fully-protective chemically synthesised vaccine development

Determining immune protection-inducing protein structures (IMPIPS) involves defining the stereo-electron and topochemical characteristics which are essential in MHC-p-TCR complex formation. Modified high activity binding peptides (mHABP) were thus synthesised to produce a large panel of IMPIPS measu...

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Autores Principales: Patarroyo, Manuel Elkin, Bermudez, Adriana, Alba, Martha Patricia, Vanegas, Magnolia, Moreno-Vranich, Armando, Poloche, Luis Antonio, Patarroyo, Manuel A.
Formato: Artículo (Article)
Lenguaje:Inglés (English)
Publicado: 2015
Materias:
Acceso en línea:http://repository.urosario.edu.co/handle/10336/19030
https://doi.org/10.1371/journal.pone.0123249
id ir-10336-19030
recordtype dspace
spelling ir-10336-190302022-08-25T21:34:30Z IMPIPS : The Immune Protection-Inducing Protein Structure concept in the search for steric-electron and topochemical principles for complete fully-protective chemically synthesised vaccine development Patarroyo, Manuel Elkin Bermudez, Adriana Alba, Martha Patricia Vanegas, Magnolia Moreno-Vranich, Armando Poloche, Luis Antonio Patarroyo, Manuel A. Proteína de unión epítopo Antígeno Hla Dr Hidrógeno Vacuna contra la malaria Nitrógeno Oxígeno Prolina Cloruro de sodio Péptido sintético Vacuna recombinante Animal Experiment Modelo animal Reacción de anticuerpo de antígeno Aoto Sitio de unión Estudio controlado Estructura de la droga Electrón Enlace de hidrógeno Estructura proteica inductora de protección inmunitaria Respuesta inmune inmunoensayo inmunogenicidad Malaria No humano Virulencia del parásito Síntesis de péptidos Química Física Plasmodium falciparum Enlace proteico Estructura de la proteína Animal Chemistry Haplorhin Conformación de proteínas Biología Inmunología Proteínas portadoras Antígenos Synthetic Vaccines Proline Synthetic Peptide Sodium Chloride Recombinant Vaccine Animal Model Animal Experiment Antigen Antibody Reaction Aotus Binding Site Controlled Study Drug Structure Electron Hydrogen Bond Immune Protection Inducing Protein Structure Immune Response Immunoassay Binding Protein Epitope Hla Dr Antigen Hydrogen Malaria Vaccine Oxygen Immunogenicity Nonhuman Parasite Virulence Peptide Synthesis Physical Chemistry Plasmodium Falciparum Protein Binding Protein Structure Chemistry Protein Conformation Haplorhini Animals Electrons Nitrogen Determining immune protection-inducing protein structures (IMPIPS) involves defining the stereo-electron and topochemical characteristics which are essential in MHC-p-TCR complex formation. Modified high activity binding peptides (mHABP) were thus synthesised to produce a large panel of IMPIPS measuring 26.5 ±3.5Å between the farthest atoms fitting into Pockets 1 to 9 of HLA-DRβ1∗structures. They displayed a polyproline II-like (PPIIL) structure with their backbone O and N atoms orientated to establish H-bonds with specific residues from HLA-DRβ∗-peptide binding regions (PBR). Residues having specific charge and gauche+ orientation regarding p3χ1, p5χ2, and p7χ1 angles determined appropriate rotamer orientation for perfectly fitting into the TCR to induce an appropriate immune response. Immunological assays in Aotus monkeys involving IMPIPS mixtures led to promising results; taken together with the aforementioned physicochemical principles, noninterfering, long-lasting, protection-inducing, multi-epitope, multistage, minimal subunit-based chemically-synthesised peptides can be designed against diseases scourging humankind. © 2015 Patarroyo et al. 2015 2019-02-08T19:45:56Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 1932-6203 http://repository.urosario.edu.co/handle/10336/19030 https://doi.org/10.1371/journal.pone.0123249 eng info:eu-repo/semantics/openAccess application/pdf instname:Universidad del Rosario
institution EdocUR - Universidad del Rosario
collection DSpace
language Inglés (English)
topic Proteína de unión
epítopo
Antígeno Hla Dr
Hidrógeno
Vacuna contra la malaria
Nitrógeno
Oxígeno
Prolina
Cloruro de sodio
Péptido sintético
Vacuna recombinante
Animal Experiment
Modelo animal
Reacción de anticuerpo de antígeno
Aoto
Sitio de unión
Estudio controlado
Estructura de la droga
Electrón
Enlace de hidrógeno
Estructura proteica inductora de protección inmunitaria
Respuesta inmune
inmunoensayo
inmunogenicidad
Malaria
No humano
Virulencia del parásito
Síntesis de péptidos
Química Física
Plasmodium falciparum
Enlace proteico
Estructura de la proteína
Animal
Chemistry
Haplorhin
Conformación de proteínas
Biología
Inmunología
Proteínas portadoras
Antígenos
Synthetic
Vaccines
Proline
Synthetic Peptide
Sodium Chloride
Recombinant Vaccine
Animal Model
Animal Experiment
Antigen Antibody Reaction
Aotus
Binding Site
Controlled Study
Drug Structure
Electron
Hydrogen Bond
Immune Protection Inducing Protein
Structure
Immune Response
Immunoassay
Binding Protein
Epitope
Hla Dr Antigen
Hydrogen
Malaria Vaccine
Oxygen
Immunogenicity
Nonhuman
Parasite Virulence
Peptide Synthesis
Physical Chemistry
Plasmodium Falciparum
Protein Binding
Protein Structure
Chemistry
Protein Conformation
Haplorhini
Animals
Electrons
Nitrogen
spellingShingle Proteína de unión
epítopo
Antígeno Hla Dr
Hidrógeno
Vacuna contra la malaria
Nitrógeno
Oxígeno
Prolina
Cloruro de sodio
Péptido sintético
Vacuna recombinante
Animal Experiment
Modelo animal
Reacción de anticuerpo de antígeno
Aoto
Sitio de unión
Estudio controlado
Estructura de la droga
Electrón
Enlace de hidrógeno
Estructura proteica inductora de protección inmunitaria
Respuesta inmune
inmunoensayo
inmunogenicidad
Malaria
No humano
Virulencia del parásito
Síntesis de péptidos
Química Física
Plasmodium falciparum
Enlace proteico
Estructura de la proteína
Animal
Chemistry
Haplorhin
Conformación de proteínas
Biología
Inmunología
Proteínas portadoras
Antígenos
Synthetic
Vaccines
Proline
Synthetic Peptide
Sodium Chloride
Recombinant Vaccine
Animal Model
Animal Experiment
Antigen Antibody Reaction
Aotus
Binding Site
Controlled Study
Drug Structure
Electron
Hydrogen Bond
Immune Protection Inducing Protein
Structure
Immune Response
Immunoassay
Binding Protein
Epitope
Hla Dr Antigen
Hydrogen
Malaria Vaccine
Oxygen
Immunogenicity
Nonhuman
Parasite Virulence
Peptide Synthesis
Physical Chemistry
Plasmodium Falciparum
Protein Binding
Protein Structure
Chemistry
Protein Conformation
Haplorhini
Animals
Electrons
Nitrogen
Patarroyo, Manuel Elkin
Bermudez, Adriana
Alba, Martha Patricia
Vanegas, Magnolia
Moreno-Vranich, Armando
Poloche, Luis Antonio
Patarroyo, Manuel A.
IMPIPS : The Immune Protection-Inducing Protein Structure concept in the search for steric-electron and topochemical principles for complete fully-protective chemically synthesised vaccine development
description Determining immune protection-inducing protein structures (IMPIPS) involves defining the stereo-electron and topochemical characteristics which are essential in MHC-p-TCR complex formation. Modified high activity binding peptides (mHABP) were thus synthesised to produce a large panel of IMPIPS measuring 26.5 ±3.5Å between the farthest atoms fitting into Pockets 1 to 9 of HLA-DRβ1∗structures. They displayed a polyproline II-like (PPIIL) structure with their backbone O and N atoms orientated to establish H-bonds with specific residues from HLA-DRβ∗-peptide binding regions (PBR). Residues having specific charge and gauche+ orientation regarding p3χ1, p5χ2, and p7χ1 angles determined appropriate rotamer orientation for perfectly fitting into the TCR to induce an appropriate immune response. Immunological assays in Aotus monkeys involving IMPIPS mixtures led to promising results; taken together with the aforementioned physicochemical principles, noninterfering, long-lasting, protection-inducing, multi-epitope, multistage, minimal subunit-based chemically-synthesised peptides can be designed against diseases scourging humankind. © 2015 Patarroyo et al.
format Artículo (Article)
author Patarroyo, Manuel Elkin
Bermudez, Adriana
Alba, Martha Patricia
Vanegas, Magnolia
Moreno-Vranich, Armando
Poloche, Luis Antonio
Patarroyo, Manuel A.
author_facet Patarroyo, Manuel Elkin
Bermudez, Adriana
Alba, Martha Patricia
Vanegas, Magnolia
Moreno-Vranich, Armando
Poloche, Luis Antonio
Patarroyo, Manuel A.
author_sort Patarroyo, Manuel Elkin
title IMPIPS : The Immune Protection-Inducing Protein Structure concept in the search for steric-electron and topochemical principles for complete fully-protective chemically synthesised vaccine development
title_short IMPIPS : The Immune Protection-Inducing Protein Structure concept in the search for steric-electron and topochemical principles for complete fully-protective chemically synthesised vaccine development
title_full IMPIPS : The Immune Protection-Inducing Protein Structure concept in the search for steric-electron and topochemical principles for complete fully-protective chemically synthesised vaccine development
title_fullStr IMPIPS : The Immune Protection-Inducing Protein Structure concept in the search for steric-electron and topochemical principles for complete fully-protective chemically synthesised vaccine development
title_full_unstemmed IMPIPS : The Immune Protection-Inducing Protein Structure concept in the search for steric-electron and topochemical principles for complete fully-protective chemically synthesised vaccine development
title_sort impips : the immune protection-inducing protein structure concept in the search for steric-electron and topochemical principles for complete fully-protective chemically synthesised vaccine development
publishDate 2015
url http://repository.urosario.edu.co/handle/10336/19030
https://doi.org/10.1371/journal.pone.0123249
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