Blastocystis subtyping and its association with intestinal parasites in children from different geographical regions of Colombia
Blastocystis is a common enteric protist colonizing probably more than 1 billion people with a large variety of non-human hosts. Remarkable genetic diversity has been observed, leading to the subdivision of the genus into multiple subtypes (ST), some of which are exclusively found in non-human hosts...
Autores Principales: | , , , , |
---|---|
Formato: | Artículo (Article) |
Lenguaje: | Inglés (English) |
Publicado: |
2017
|
Materias: | |
Acceso en línea: | http://repository.urosario.edu.co/handle/10336/18795 https://doi.org/10.1371/journal.pone.0172586 |
id |
ir-10336-18795 |
---|---|
recordtype |
dspace |
institution |
EdocUR - Universidad del Rosario |
collection |
DSpace |
language |
Inglés (English) |
topic |
Dolor abdominal Protozoario Ribosomal Taxonómico Adolescente Viejo que pica Lombriz intestinal Infección asintomática Blastocistosis Niño Estudio controlado Diarrea Código de barras de ADN Madre endolímax Entamoeba Entamoeba Coli Secuencia de genes Distribución geográfica Giardia intestinalis Dolor de cabeza Humano Himenolepis nana Parásito del intestino Masculino Diagnóstico Molecular Tipificación molecular Nausea Asesino americano Neobalantidium Coli No humano Secuencia de nucleótidos Reacción en cadena de la polimerasa en tiempo real Vómitos Enfermedad asintomática Infecciones por blastocistis Clasificación Estudio comparativo Código de barras de ADN Heces Variación genética Genética Encuesta de Salud Infección intestinal Aislamiento y Purificación Geografía Médica Infección mixta Parasitología Niño preescolar Ribotipado Diferencia de especies ADN protozoario ARN protozoario ADN ribosomal Rna 18S Adolescente Alelos Enfermedades Asintomáticas Infecciones por blastocistis Coinfección ADN Reacción en cadena de la polimerasa en tiempo real Ribotipado ARN, Ribosomal, 18S Especificidad de especie Infecciones por Blastocystis Blastocystis Gusanos parásitos e intestinales Medical Parasitic Preschool Protozoan Species Specificity Blastocystis Female Rna, Ribosomal, 18S Ribotyping Child Geography Intestinal Diseases RNA Real-Time Polymerase Chain DNA Coinfection Blastocystis Infections Alleles Asymptomatic Diseases Adolescent Ribosome Dna Protozoal Dna Protozoal Rna Species Difference Ribotyping Preschool Child Parasitolog Mixed Infection Medical Geography Isolation And Purification Intestine Infection Health Survey Genetics Variación genética Genetic Variation Feces Dna Barcoding Comparative Study Classification Blastocystis Infections Asymptomatic Disease Vomiting Reaction Real Time Polymerase Chain Nucleotide Sequence Necator Americanus Nonhuman Molecular Typing Molecular Diagnosis Intestine Parasite Male Hymenolepis Nana Human Headache Geographic Distribution Gene Sequence Endolimax Nana Dna Barcoding Controlled Study Blastocystosis Asymptomatic Infection Asymptomatic Infection Anus Pruritus Ancylostoma Duodenale Taxonomic Adolescent Protozoan Abdominal Pain Diarrhea Reaction Humans Allele |
spellingShingle |
Dolor abdominal Protozoario Ribosomal Taxonómico Adolescente Viejo que pica Lombriz intestinal Infección asintomática Blastocistosis Niño Estudio controlado Diarrea Código de barras de ADN Madre endolímax Entamoeba Entamoeba Coli Secuencia de genes Distribución geográfica Giardia intestinalis Dolor de cabeza Humano Himenolepis nana Parásito del intestino Masculino Diagnóstico Molecular Tipificación molecular Nausea Asesino americano Neobalantidium Coli No humano Secuencia de nucleótidos Reacción en cadena de la polimerasa en tiempo real Vómitos Enfermedad asintomática Infecciones por blastocistis Clasificación Estudio comparativo Código de barras de ADN Heces Variación genética Genética Encuesta de Salud Infección intestinal Aislamiento y Purificación Geografía Médica Infección mixta Parasitología Niño preescolar Ribotipado Diferencia de especies ADN protozoario ARN protozoario ADN ribosomal Rna 18S Adolescente Alelos Enfermedades Asintomáticas Infecciones por blastocistis Coinfección ADN Reacción en cadena de la polimerasa en tiempo real Ribotipado ARN, Ribosomal, 18S Especificidad de especie Infecciones por Blastocystis Blastocystis Gusanos parásitos e intestinales Medical Parasitic Preschool Protozoan Species Specificity Blastocystis Female Rna, Ribosomal, 18S Ribotyping Child Geography Intestinal Diseases RNA Real-Time Polymerase Chain DNA Coinfection Blastocystis Infections Alleles Asymptomatic Diseases Adolescent Ribosome Dna Protozoal Dna Protozoal Rna Species Difference Ribotyping Preschool Child Parasitolog Mixed Infection Medical Geography Isolation And Purification Intestine Infection Health Survey Genetics Variación genética Genetic Variation Feces Dna Barcoding Comparative Study Classification Blastocystis Infections Asymptomatic Disease Vomiting Reaction Real Time Polymerase Chain Nucleotide Sequence Necator Americanus Nonhuman Molecular Typing Molecular Diagnosis Intestine Parasite Male Hymenolepis Nana Human Headache Geographic Distribution Gene Sequence Endolimax Nana Dna Barcoding Controlled Study Blastocystosis Asymptomatic Infection Asymptomatic Infection Anus Pruritus Ancylostoma Duodenale Taxonomic Adolescent Protozoan Abdominal Pain Diarrhea Reaction Humans Allele Ramírez, Juan David Flórez, Carolina Olivera, Mario Bernal, María Consuelo Giraldo, Julio Cesar Blastocystis subtyping and its association with intestinal parasites in children from different geographical regions of Colombia |
description |
Blastocystis is a common enteric protist colonizing probably more than 1 billion people with a large variety of non-human hosts. Remarkable genetic diversity has been observed, leading to the subdivision of the genus into multiple subtypes (ST), some of which are exclusively found in non-human hosts. The aim of this study was to determine the distribution of Blastocystis STs/18S alleles in symptomatic (abdominal pain, anal pruritus, diarrhea, headache, nauseas and/or vomit) and asymptomatic children from nine geographical regions of Colombia. A total of 2026 fecal samples were collected as part of a national survey to estimate the frequency of intestinal parasites in children. A set of 256 samples that were Blastocystis positive was finally selected. The samples were submitted to DNA extraction, Real Time PCR and sequencing using Blastocystis-specific primers targeting the small subunit rRNA gene for ST identification. DNA of Ascaris lumbricoides (16.4%), Trichuris trichiura (8.2%), hookworms (Necator americanus/Ancylostoma duodenale) (7.3%), Giardia duodenalis (23.1%), Entamoeba complex (82%), Entamoeba coli (55%), Hymenolepis nana (0.8%), Endolimax nana (33.2%) and Neobalantidium coli (2.7%) was detected in the Blastocystispositive samples. We detected ST1 (21.4%), ST2 (19.5%), ST3 (55.5%), ST4 (0.8%), ST6 (2%) and ST7 (0.8%); alleles 1, 2, 4, 81, 82 and 83 for ST1; alleles 9, 11, 12, 15, 67, 71 and 73 for ST2; alleles 34, 36, 38, 45, 49, 55, 134 and 128 for ST3; allele 42 for ST4; allele 122 for ST6, and allele 142 for ST7. Further studies implementing high-resolution molecular markers are necessary to understand the dynamics of Blastocystis transmission and the role of this Stramenopila in health and disease. © 2017 Ramírez et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
format |
Artículo (Article) |
author |
Ramírez, Juan David Flórez, Carolina Olivera, Mario Bernal, María Consuelo Giraldo, Julio Cesar |
author_facet |
Ramírez, Juan David Flórez, Carolina Olivera, Mario Bernal, María Consuelo Giraldo, Julio Cesar |
author_sort |
Ramírez, Juan David |
title |
Blastocystis subtyping and its association with intestinal parasites in children from different geographical regions of Colombia |
title_short |
Blastocystis subtyping and its association with intestinal parasites in children from different geographical regions of Colombia |
title_full |
Blastocystis subtyping and its association with intestinal parasites in children from different geographical regions of Colombia |
title_fullStr |
Blastocystis subtyping and its association with intestinal parasites in children from different geographical regions of Colombia |
title_full_unstemmed |
Blastocystis subtyping and its association with intestinal parasites in children from different geographical regions of Colombia |
title_sort |
blastocystis subtyping and its association with intestinal parasites in children from different geographical regions of colombia |
publishDate |
2017 |
url |
http://repository.urosario.edu.co/handle/10336/18795 https://doi.org/10.1371/journal.pone.0172586 |
_version_ |
1743430932900085760 |
spelling |
ir-10336-187952022-08-27T18:56:26Z Blastocystis subtyping and its association with intestinal parasites in children from different geographical regions of Colombia Ramírez, Juan David Flórez, Carolina Olivera, Mario Bernal, María Consuelo Giraldo, Julio Cesar Dolor abdominal Protozoario Ribosomal Taxonómico Adolescente Viejo que pica Lombriz intestinal Infección asintomática Blastocistosis Niño Estudio controlado Diarrea Código de barras de ADN Madre endolímax Entamoeba Entamoeba Coli Secuencia de genes Distribución geográfica Giardia intestinalis Dolor de cabeza Humano Himenolepis nana Parásito del intestino Masculino Diagnóstico Molecular Tipificación molecular Nausea Asesino americano Neobalantidium Coli No humano Secuencia de nucleótidos Reacción en cadena de la polimerasa en tiempo real Vómitos Enfermedad asintomática Infecciones por blastocistis Clasificación Estudio comparativo Código de barras de ADN Heces Variación genética Genética Encuesta de Salud Infección intestinal Aislamiento y Purificación Geografía Médica Infección mixta Parasitología Niño preescolar Ribotipado Diferencia de especies ADN protozoario ARN protozoario ADN ribosomal Rna 18S Adolescente Alelos Enfermedades Asintomáticas Infecciones por blastocistis Coinfección ADN Reacción en cadena de la polimerasa en tiempo real Ribotipado ARN, Ribosomal, 18S Especificidad de especie Infecciones por Blastocystis Blastocystis Gusanos parásitos e intestinales Medical Parasitic Preschool Protozoan Species Specificity Blastocystis Female Rna, Ribosomal, 18S Ribotyping Child Geography Intestinal Diseases RNA Real-Time Polymerase Chain DNA Coinfection Blastocystis Infections Alleles Asymptomatic Diseases Adolescent Ribosome Dna Protozoal Dna Protozoal Rna Species Difference Ribotyping Preschool Child Parasitolog Mixed Infection Medical Geography Isolation And Purification Intestine Infection Health Survey Genetics Variación genética Genetic Variation Feces Dna Barcoding Comparative Study Classification Blastocystis Infections Asymptomatic Disease Vomiting Reaction Real Time Polymerase Chain Nucleotide Sequence Necator Americanus Nonhuman Molecular Typing Molecular Diagnosis Intestine Parasite Male Hymenolepis Nana Human Headache Geographic Distribution Gene Sequence Endolimax Nana Dna Barcoding Controlled Study Blastocystosis Asymptomatic Infection Asymptomatic Infection Anus Pruritus Ancylostoma Duodenale Taxonomic Adolescent Protozoan Abdominal Pain Diarrhea Reaction Humans Allele Blastocystis is a common enteric protist colonizing probably more than 1 billion people with a large variety of non-human hosts. Remarkable genetic diversity has been observed, leading to the subdivision of the genus into multiple subtypes (ST), some of which are exclusively found in non-human hosts. The aim of this study was to determine the distribution of Blastocystis STs/18S alleles in symptomatic (abdominal pain, anal pruritus, diarrhea, headache, nauseas and/or vomit) and asymptomatic children from nine geographical regions of Colombia. A total of 2026 fecal samples were collected as part of a national survey to estimate the frequency of intestinal parasites in children. A set of 256 samples that were Blastocystis positive was finally selected. The samples were submitted to DNA extraction, Real Time PCR and sequencing using Blastocystis-specific primers targeting the small subunit rRNA gene for ST identification. DNA of Ascaris lumbricoides (16.4%), Trichuris trichiura (8.2%), hookworms (Necator americanus/Ancylostoma duodenale) (7.3%), Giardia duodenalis (23.1%), Entamoeba complex (82%), Entamoeba coli (55%), Hymenolepis nana (0.8%), Endolimax nana (33.2%) and Neobalantidium coli (2.7%) was detected in the Blastocystispositive samples. We detected ST1 (21.4%), ST2 (19.5%), ST3 (55.5%), ST4 (0.8%), ST6 (2%) and ST7 (0.8%); alleles 1, 2, 4, 81, 82 and 83 for ST1; alleles 9, 11, 12, 15, 67, 71 and 73 for ST2; alleles 34, 36, 38, 45, 49, 55, 134 and 128 for ST3; allele 42 for ST4; allele 122 for ST6, and allele 142 for ST7. Further studies implementing high-resolution molecular markers are necessary to understand the dynamics of Blastocystis transmission and the role of this Stramenopila in health and disease. © 2017 Ramírez et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 2017-02-21 2018-12-10T22:43:11Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion ISSN 1932-6203 http://repository.urosario.edu.co/handle/10336/18795 https://doi.org/10.1371/journal.pone.0172586 eng info:eu-repo/semantics/openAccess application/pdf instname:Universidad del Rosario |
score |
12,131701 |