Vista Equipo: Profiling of gene expression regulated by 17β-estradiol and tamoxifen in estrogen receptor-positive and estrogen receptor-negative human breast cancer cell lines

Profiling of gene expression regulated by 17β-estradiol and tamoxifen in estrogen receptor-positive and estrogen receptor-negative human breast cancer cell lines

One area of great importance in breast cancer (BC) research is the study of gene expression regulated by both estrogenic and antiestrogenic agents. Although many studies have been performed in this area, most of them have only addressed the effects of 17β-estradiol (E2) and tamoxifen (TAM) on MCF7 c...

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Autores Principales:	Rangel, Nelson, Villegas, Victoria E., Rondón-Lagos, Milena
Formato:	Artículo (Article)
Lenguaje:	Inglés (English)
Publicado:	2017
Materias:	17Β-Estradiol Breast Cancer Cell Lines Erα+ Erα− Qpcr Tamoxifen Neoplasmas Carcinogénesis Tamoxifeno Estradiol Mucin 1 Apoptosis Article Bc Cell Line Birc5 Gene Carcinogenesis Cell Culture Cell Cycle Progression Cell Proliferation Cell Survival Cell Transformation Controlled Study Dna Damage Dna Fingerprinting Estrogen Receptor Negative Breast Cancer Estrogen Receptor Positive Breast Cancer Gene Gene Expression Human Human Cell Mcf-7 Cell Line Metastasis Mucin 1 Gene Real Time Polymerase Chain Reaction Reverse Transcription Polymerase Chain Reaction
Acceso en línea:	http://repository.urosario.edu.co/handle/10336/18708

id	ir-10336-18708
recordtype	dspace
spelling	ir-10336-187082019-09-19T12:38:03Z Profiling of gene expression regulated by 17β-estradiol and tamoxifen in estrogen receptor-positive and estrogen receptor-negative human breast cancer cell lines Rangel, Nelson Villegas, Victoria E. Rondón-Lagos, Milena 17Β-Estradiol Breast Cancer Cell Lines Erα+ Erα− Qpcr Tamoxifen Neoplasmas Carcinogénesis Tamoxifeno Estradiol Mucin 1 Tamoxifen Apoptosis Article Bc Cell Line Birc5 Gene Carcinogenesis Cell Culture Cell Cycle Progression Cell Proliferation Cell Survival Cell Transformation Controlled Study Dna Damage Dna Fingerprinting Estrogen Receptor Negative Breast Cancer Estrogen Receptor Positive Breast Cancer Gene Gene Expression Human Human Cell Mcf-7 Cell Line Metastasis Mucin 1 Gene Real Time Polymerase Chain Reaction Reverse Transcription Polymerase Chain Reaction One area of great importance in breast cancer (BC) research is the study of gene expression regulated by both estrogenic and antiestrogenic agents. Although many studies have been performed in this area, most of them have only addressed the effects of 17β-estradiol (E2) and tamoxifen (TAM) on MCF7 cells. This study aimed to determine the effect of low doses of E2 and TAM on the expression levels of 84 key genes, which are commonly involved in breast carcinogenesis, in four BC cell lines differentially expressing estrogen receptor (ER) α and HER2 (MCF7, T47D, BT474, and SKBR3). The results allowed us to determine the expression patterns modulated by E2 and TAM in ERα+ and ERα− cell lines, as well as to identify differences in expression patterns. Although the MCF7 cell line is the most frequently used model to determine gene expression profiles in response to E2 and TAM, the changes in gene expression patterns identified in ERα+ and ERα− cell lines could reflect distinctive properties of these cells. Our results could provide important markers to be validated in BC patient samples, and subsequently used for predicting the outcome in ERα+ and ERα− tumors after TAM or hormonal therapy. Considering that BC is a molecularly heterogeneous disease, it is important to understand how well, and which cell lines, best model that diversity. © 2017 Rangel et al. 2017 2018-11-19T21:31:11Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion ISSN 1179-1314 http://repository.urosario.edu.co/handle/10336/18708 eng info:eu-repo/semantics/openAccess application/pdf Ferlay, J., Soerjomataram, I., Ervik, M., (2013) GLOBOCAN 2012 V1.0, Cancer Incidence and Mortality Worldwide, , http://globocan.iarc.fr, IARC cancer base no. 11 [Internet]. Lyon: IARC
institution	EdocUR - Universidad del Rosario
collection	DSpace
language	Inglés (English)
topic	17Β-Estradiol Breast Cancer Cell Lines Erα+ Erα− Qpcr Tamoxifen Neoplasmas Carcinogénesis Tamoxifeno Estradiol Mucin 1 Tamoxifen Apoptosis Article Bc Cell Line Birc5 Gene Carcinogenesis Cell Culture Cell Cycle Progression Cell Proliferation Cell Survival Cell Transformation Controlled Study Dna Damage Dna Fingerprinting Estrogen Receptor Negative Breast Cancer Estrogen Receptor Positive Breast Cancer Gene Gene Expression Human Human Cell Mcf-7 Cell Line Metastasis Mucin 1 Gene Real Time Polymerase Chain Reaction Reverse Transcription Polymerase Chain Reaction
spellingShingle	17Β-Estradiol Breast Cancer Cell Lines Erα+ Erα− Qpcr Tamoxifen Neoplasmas Carcinogénesis Tamoxifeno Estradiol Mucin 1 Tamoxifen Apoptosis Article Bc Cell Line Birc5 Gene Carcinogenesis Cell Culture Cell Cycle Progression Cell Proliferation Cell Survival Cell Transformation Controlled Study Dna Damage Dna Fingerprinting Estrogen Receptor Negative Breast Cancer Estrogen Receptor Positive Breast Cancer Gene Gene Expression Human Human Cell Mcf-7 Cell Line Metastasis Mucin 1 Gene Real Time Polymerase Chain Reaction Reverse Transcription Polymerase Chain Reaction Rangel, Nelson Villegas, Victoria E. Rondón-Lagos, Milena Profiling of gene expression regulated by 17β-estradiol and tamoxifen in estrogen receptor-positive and estrogen receptor-negative human breast cancer cell lines
description	One area of great importance in breast cancer (BC) research is the study of gene expression regulated by both estrogenic and antiestrogenic agents. Although many studies have been performed in this area, most of them have only addressed the effects of 17β-estradiol (E2) and tamoxifen (TAM) on MCF7 cells. This study aimed to determine the effect of low doses of E2 and TAM on the expression levels of 84 key genes, which are commonly involved in breast carcinogenesis, in four BC cell lines differentially expressing estrogen receptor (ER) α and HER2 (MCF7, T47D, BT474, and SKBR3). The results allowed us to determine the expression patterns modulated by E2 and TAM in ERα+ and ERα− cell lines, as well as to identify differences in expression patterns. Although the MCF7 cell line is the most frequently used model to determine gene expression profiles in response to E2 and TAM, the changes in gene expression patterns identified in ERα+ and ERα− cell lines could reflect distinctive properties of these cells. Our results could provide important markers to be validated in BC patient samples, and subsequently used for predicting the outcome in ERα+ and ERα− tumors after TAM or hormonal therapy. Considering that BC is a molecularly heterogeneous disease, it is important to understand how well, and which cell lines, best model that diversity. © 2017 Rangel et al.
format	Artículo (Article)
author	Rangel, Nelson Villegas, Victoria E. Rondón-Lagos, Milena
author_facet	Rangel, Nelson Villegas, Victoria E. Rondón-Lagos, Milena
author_sort	Rangel, Nelson
title	Profiling of gene expression regulated by 17β-estradiol and tamoxifen in estrogen receptor-positive and estrogen receptor-negative human breast cancer cell lines
title_short	Profiling of gene expression regulated by 17β-estradiol and tamoxifen in estrogen receptor-positive and estrogen receptor-negative human breast cancer cell lines
title_full	Profiling of gene expression regulated by 17β-estradiol and tamoxifen in estrogen receptor-positive and estrogen receptor-negative human breast cancer cell lines
title_fullStr	Profiling of gene expression regulated by 17β-estradiol and tamoxifen in estrogen receptor-positive and estrogen receptor-negative human breast cancer cell lines
title_full_unstemmed	Profiling of gene expression regulated by 17β-estradiol and tamoxifen in estrogen receptor-positive and estrogen receptor-negative human breast cancer cell lines
title_sort	profiling of gene expression regulated by 17β-estradiol and tamoxifen in estrogen receptor-positive and estrogen receptor-negative human breast cancer cell lines
publishDate	2017
url	http://repository.urosario.edu.co/handle/10336/18708
_version_	1651341387395760128
score	12,352018

Profiling of gene expression regulated by 17β-estradiol and tamoxifen in estrogen receptor-positive and estrogen receptor-negative human breast cancer cell lines

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