In vitro and in vivo studies for assessing the immune response and protection-inducing ability conferred by Fasciola hepatica-derived synthetic peptides containing B- and T-cell epitopes
Fasciolosis is considered the most widespread trematode disease affecting grazing animals around the world; it is currently recognised by the World Health Organisation as an emergent human pathogen. Triclabendazole is still the most effective drug against this disease; however, resistant strains hav...
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| Formato: | Artículo (Article) |
| Lenguaje: | Inglés (English) |
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2014
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| Acceso en línea: | http://repository.urosario.edu.co/handle/10336/18685 https://doi.org/10.1371/journal.pone.0105323 |
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ir-10336-186852022-08-29T15:44:53Z In vitro and in vivo studies for assessing the immune response and protection-inducing ability conferred by Fasciola hepatica-derived synthetic peptides containing B- and T-cell epitopes Rojas-Caraballo, Jose López-Abán, Julio Pérez del Villa, Luis Vizcaíno, Carolina Vicente, Belén Fernández-Soto, Pedro del Olmo, Esther Alfonso Patarroyo, Manuel Muro, Antonio Cytokine Epitope Helminth Antibody Immunoglobulin G Peptide Protozoal Vaccine Amino Acid Sequence Animal Blood Chemistry Cluster Analysis Disease Model Fasciola Hepatica Fascioliasis Female Gene Expression Profiling Genetics Immunology Metabolism Mortality Mouse Parasitology Synthesis Amino Acid Sequence Animals Fasciola Hepatica Fascioliasis Gene Expression Profiling Mice Peptides Protozoan Vaccines Animal T-Lymphocyte B-Lymphocyte Disease Models Epitopes Epitopes Fasciola hepática Enzimología Parasitología Fasciolosis is considered the most widespread trematode disease affecting grazing animals around the world; it is currently recognised by the World Health Organisation as an emergent human pathogen. Triclabendazole is still the most effective drug against this disease; however, resistant strains have appeared and developing an effective vaccine against this disease has increasingly become a priority. Several bioinformatics tools were here used for predicting B- and T-cell epitopes according to the available data for Fasciola hepatica protein amino acid sequences. BALB/c mice were immunised with the synthetic peptides by using the ADAD vaccination system and several immune response parameters were measured (antibody titres, cytokine levels, T-cell populations) to evaluate their ability to elicit an immune response. Based on the immunogenicity results so obtained, seven peptides were selected to assess their protection-inducing ability against experimental infection with F. hepatica metacercariae. Twenty-four B- or T-epitope-containing peptides were predicted and chemically synthesised. Immunisation of mice with peptides so-called B1, B2, B5, B6, T14, T15 and T16 induced high levels of total IgG, IgG1 and IgG2a (p<0.05) and a mixed Th1/Th2/Th17/Treg immune response, according to IFN-γ, IL-4, IL-17 and IL-10 levels, accompanied by increased CD62L+ T-cell populations. A high level of protection was obtained in mice vaccinated with peptides B2, B5, B6 and T15 formulated in the ADAD vaccination system with the AA0029 immunomodulator. The bioinformatics approach used in the present study led to the identification of seven peptides as vaccine candidates against the infection caused by Fasciola hepatica (a liver-fluke trematode). However, vaccine efficacy must be evaluated in other host species, including those having veterinary importance. © 2014 Rojas-Caraballo et al. 2014 2018-11-07T16:00:25Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 1932-6203 http://repository.urosario.edu.co/handle/10336/18685 https://doi.org/10.1371/journal.pone.0105323 eng info:eu-repo/semantics/openAccess application/pdf instname:Universidad del Rosario |
| institution |
EdocUR - Universidad del Rosario |
| collection |
DSpace |
| language |
Inglés (English) |
| topic |
Cytokine Epitope Helminth Antibody Immunoglobulin G Peptide Protozoal Vaccine Amino Acid Sequence Animal Blood Chemistry Cluster Analysis Disease Model Fasciola Hepatica Fascioliasis Female Gene Expression Profiling Genetics Immunology Metabolism Mortality Mouse Parasitology Synthesis Amino Acid Sequence Animals Fasciola Hepatica Fascioliasis Gene Expression Profiling Mice Peptides Protozoan Vaccines Animal T-Lymphocyte B-Lymphocyte Disease Models Epitopes Epitopes Fasciola hepática Enzimología Parasitología |
| spellingShingle |
Cytokine Epitope Helminth Antibody Immunoglobulin G Peptide Protozoal Vaccine Amino Acid Sequence Animal Blood Chemistry Cluster Analysis Disease Model Fasciola Hepatica Fascioliasis Female Gene Expression Profiling Genetics Immunology Metabolism Mortality Mouse Parasitology Synthesis Amino Acid Sequence Animals Fasciola Hepatica Fascioliasis Gene Expression Profiling Mice Peptides Protozoan Vaccines Animal T-Lymphocyte B-Lymphocyte Disease Models Epitopes Epitopes Fasciola hepática Enzimología Parasitología Rojas-Caraballo, Jose López-Abán, Julio Pérez del Villa, Luis Vizcaíno, Carolina Vicente, Belén Fernández-Soto, Pedro del Olmo, Esther Alfonso Patarroyo, Manuel Muro, Antonio In vitro and in vivo studies for assessing the immune response and protection-inducing ability conferred by Fasciola hepatica-derived synthetic peptides containing B- and T-cell epitopes |
| description |
Fasciolosis is considered the most widespread trematode disease affecting grazing animals around the world; it is currently recognised by the World Health Organisation as an emergent human pathogen. Triclabendazole is still the most effective drug against this disease; however, resistant strains have appeared and developing an effective vaccine against this disease has increasingly become a priority. Several bioinformatics tools were here used for predicting B- and T-cell epitopes according to the available data for Fasciola hepatica protein amino acid sequences. BALB/c mice were immunised with the synthetic peptides by using the ADAD vaccination system and several immune response parameters were measured (antibody titres, cytokine levels, T-cell populations) to evaluate their ability to elicit an immune response. Based on the immunogenicity results so obtained, seven peptides were selected to assess their protection-inducing ability against experimental infection with F. hepatica metacercariae. Twenty-four B- or T-epitope-containing peptides were predicted and chemically synthesised. Immunisation of mice with peptides so-called B1, B2, B5, B6, T14, T15 and T16 induced high levels of total IgG, IgG1 and IgG2a (p<0.05) and a mixed Th1/Th2/Th17/Treg immune response, according to IFN-γ, IL-4, IL-17 and IL-10 levels, accompanied by increased CD62L+ T-cell populations. A high level of protection was obtained in mice vaccinated with peptides B2, B5, B6 and T15 formulated in the ADAD vaccination system with the AA0029 immunomodulator. The bioinformatics approach used in the present study led to the identification of seven peptides as vaccine candidates against the infection caused by Fasciola hepatica (a liver-fluke trematode). However, vaccine efficacy must be evaluated in other host species, including those having veterinary importance. © 2014 Rojas-Caraballo et al. |
| format |
Artículo (Article) |
| author |
Rojas-Caraballo, Jose López-Abán, Julio Pérez del Villa, Luis Vizcaíno, Carolina Vicente, Belén Fernández-Soto, Pedro del Olmo, Esther Alfonso Patarroyo, Manuel Muro, Antonio |
| author_facet |
Rojas-Caraballo, Jose López-Abán, Julio Pérez del Villa, Luis Vizcaíno, Carolina Vicente, Belén Fernández-Soto, Pedro del Olmo, Esther Alfonso Patarroyo, Manuel Muro, Antonio |
| author_sort |
Rojas-Caraballo, Jose |
| title |
In vitro and in vivo studies for assessing the immune response and protection-inducing ability conferred by Fasciola hepatica-derived synthetic peptides containing B- and T-cell epitopes |
| title_short |
In vitro and in vivo studies for assessing the immune response and protection-inducing ability conferred by Fasciola hepatica-derived synthetic peptides containing B- and T-cell epitopes |
| title_full |
In vitro and in vivo studies for assessing the immune response and protection-inducing ability conferred by Fasciola hepatica-derived synthetic peptides containing B- and T-cell epitopes |
| title_fullStr |
In vitro and in vivo studies for assessing the immune response and protection-inducing ability conferred by Fasciola hepatica-derived synthetic peptides containing B- and T-cell epitopes |
| title_full_unstemmed |
In vitro and in vivo studies for assessing the immune response and protection-inducing ability conferred by Fasciola hepatica-derived synthetic peptides containing B- and T-cell epitopes |
| title_sort |
in vitro and in vivo studies for assessing the immune response and protection-inducing ability conferred by fasciola hepatica-derived synthetic peptides containing b- and t-cell epitopes |
| publishDate |
2014 |
| url |
http://repository.urosario.edu.co/handle/10336/18685 https://doi.org/10.1371/journal.pone.0105323 |
| _version_ |
1743431047139295232 |
| score |
12,131701 |